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Interaction of Monomers in Near-Infrared Fluorescent Biomarkers

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Abstract

Here, we analyze how the inter-monomeric interaction in the near-infrared fluorescent boimarkers iRFP713 and iRFP713/C15S/V256C is affected by the rearrangement of the hydrogen bond network between the chromophore and the adjacent amino acids and bound water molecules as result of amino acid substitution of threonine at position 204 for alanine (T204A) in its local environment or replacement of natural ligand biliverdin with phycocyanobilin. Previously found allosteric inhibition of covalent binding of the biliverdin to a monomer of iRFP713/C15S/V256C after covalent binding of the chromophore to another monomer is markedly reduced in the protein with T204A substitution. There is no allosteric inhibition of covalent binding of phycocyanobilin to iRFP713/C15S/V256C, in contrast to the binding of biliverdin to this protein. Contrary, the replacement of biliverdin with phycocyanobilin in iRFP713 leads to increased allosteric inhibition of covalent chromophore binding. Our studies indicate that the change in the intramolecular contacts involving the chromophore and its protein environment in biomarkers caused by chromophore replacement or amino acid substitutions influences allosteric communication between monomers of the biomarker.

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Funding

This work was supported by the Russian Foundation for Basic Research (project no. 16-04-01515-a).

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Authors and Affiliations

  1. Institute of Cytology, Russian Academy of Sciences, 194064, St. Petersburg, Russia

    Olga V. Stepanenko & Olesya V. Stepanenko

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  1. Olga V. Stepanenko
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  2. Olesya V. Stepanenko
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Correspondence to Olga V. Stepanenko.

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The authors declare no conflict of interest.

This article does not contain any studies involving human participants and/or animals performed by any of the authors.

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Abbreviations: NIR biomarkers—near-infrared fluorescent biomarkers, BV—biliverdin, PCB—phycocyanobilin, GdnHCl— guanidine hydrochloride.

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Stepanenko, O.V., Stepanenko, O.V. Interaction of Monomers in Near-Infrared Fluorescent Biomarkers. Cell Tiss. Biol. 15, 310–315 (2021). https://doi.org/10.1134/S1990519X21030123

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