Abstract
The karyotype of Amoeba borokensis was studied for the first time. At the metaphase of mitosis, this species has a haploid set of chromosomes (n = 27). This is exactly half of the diploid karyotype in Amoeba proteus (strain B). At first glance, it confirms the idea that has recently appeared that one species arises from another via multiple changes in chromosome number. However, comparative cytogenetic analysis revealed that four orthologous chromosomes from haploid sets of these two species were not distinguished by the pattern of DAPI-stained bands. It shows that the genetic distance between A. proteus and A. borokensis is higher than was believed earlier and these two species have diverged from each other. Analysis of data on the life cycles of A. proteus and A. borokensis shows that a kind of so-called “cyclic polyploidy” takes place in “proteus-like” amoebae. “Cyclic polyploidy” has recently been considered as an alternative to the sexual process for genetic recombination in agamic protists from different macrotaxons.
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References
Afon’kin, S.Yu., The maintenance of DNA in nuclei of different strains of Amoeba proteus according to a cytofluorometry, Tsitologiia, 1983, vol. 25, no. 7, pp. 771–776.
Afon’kin, S.Yu., Induced and spontaneous polyploidization in large amoebae, Int. Rev. Cytol., 1989, vol. 115, pp. 231–266.
Demin, S., Pleskach, N., Svetlova, M., and Solovyeva, L., High-resolution mapping of interstitial telomeric repeats in Syrian hamster metaphase chromosomes, Cytogenet. Genome Res., 2011, vol. 132, pp. 151–155.
Demin, S.Yu., Berdieva, M.A., Podlipaeva, Yu.I., Yudin, A.L., and Goodkov, A.V., Optical tomography analysis of Amoeba proteus chromatin organization at various cell cycle stages, Cell Tissue Biol., 2016, vol. 10, pp. 84–94.
Demin, S.Yu., Berdieva, M.A., Podlipaeva, Yu.I., Yudin, A.L., and Goodkov, A.V., Karyotyping of Amoeba proteus, Cell Tissue Biol., 2017, vol. 11, pp. 308–313.
Goodkov, A.V., Yudin, A.L., and Podlipaeva, Yu.I., Collection of the proteus-type amoebae at the Institute of Cytology, Russian Academy of Sciences. I. History, goals and research field, Protistology, 2014, vol. 8, no. 2, pp. 71–75.
Goodkov, A.V., Yudin, A.L., and Podlipaeva, Yu.I., Collection of the proteus-type amoebae at the Institute of Cytology, Russian Academy of Sciences. II. Index of strains and list of publications, Protistology, 2015, vol. 9, no. 2, pp. 99–111.
Kalinina, L.V., Afon’kin, S.Yu., Gromov, D.B., Khrebtukova, I.A., and Page, F.C., Amoeba borokensis n. sp., a rapidly dividing organism especially suitable for experimental purposes, Arch. Protistenk., 1986, vol. 132, pp. 343–361.
Kapuscinski, J., DAPI: a DNA-specific fluorescent probe, Biotech. Histochem., 1995, vol. 70, pp. 220–233.
Kondrashov, A.S., The asexual ploidy cycle and the origin of sex, Nature, 1994, vol. 370, pp. 213–216.
Kondrashov, A.S., Evolutionary genetics of life cycles, Annu. Rev. Ecol. Syst., 1997, vol. 28, pp. 391–435.
Lahr, D.J.G., Parfrey, L.W., Mitchell, E.A.D., Katz, L.A., and Lara, E., The chastity of amoebae: re-evaluating evidence for sex in amoeboid organisms, Proc. R. Soc. B, 2011, vol. 278, pp. 2081–2090.
Makhlin, E.E., Variability of DNA quantity synthesized in Amoeba proteus nuclei during the cell cycle, Tsitologiia, 1987, vol. 29, no. 12, pp. 1379–1384.
Makhlin, E.E., The extra DNA synthesis in Amoeba proteus nuclei during the cell cycle, Tsitologiia, 1993, vol. 35, no. 3, pp. 109–121.
Makhlin, E.E., Kudryavtseva, M.V., and Kudryavtsev, B.N., Peculiarities of changes in DNA content of Amoeba proteus nuclei during interphase, Exp. Cell Res., 1979, vol. 118, pp. 143–150.
Ord, M.J., The synthesis of DNA through the cell cycle of Amoeba proteus, J. Cell Sci., 1968, vol. 3, pp. 483–491.
Parfrey, L.W., Lahr, D.J.G., and Katz, L.A., The dynamic nature of eukaryotic genomes, Mol. Biol. Evol., 2008, vol. 25, pp. 787–794.
Podlipaeva, Yu., Demin, S., and Goodkov, A., New method for cell cycle synchronization in Amoeba proteus culture, Protistology, 2013, vol. 8, pp. 3–7.
Prescott, D.M. and Carrier, R.F., Experimental procedures and cultural methods for Euplotes eurystomus and Amoeba proteus, in Methods in Cell Physiology, New York: Acad. Press, 1964, vol. 1, pp. 85–95.
Raikov, I.B., Yadro prosteishikh. Morfologiya i evolyutsiya (Protozoan Nucleus. Morphology and Evolution), Leningrad: Nauka, 1978.
Raikov, I.B., The Protozoan Nucleus, Morphology and Evolution, Wien: Springer, 1982.
Rogerson, A., Generation times and reproductive rates of Amoeba proteus (Leidy) as influenced by temperature and food concentration, Can. J. Zool., 1980, vol. 58, pp. 543–548.
Sopina, V.A., The multiplication rate of amoebae related to the cultivation temperature, J. Therm. Biol., 1976, vol. 1, 199–204.
Yudin, A.L., Amoeba, in Ob”ekty biologii razvitiya (Objects of Developmental Biology), Moscow: Nauka, 1975, pp. 5–12.
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Demin, S.Y., Podlipaeva, Y.I., Berdieva, M.A. et al. The Karyotype of Amoeba borokensis from a “proteus-like” Amoeba Group (Amoebozoa: Euamoebida). Cell Tiss. Biol. 12, 85–91 (2018). https://doi.org/10.1134/S1990519X18010054
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DOI: https://doi.org/10.1134/S1990519X18010054