Abstract
We found that the S-SAP (Sequence-Specific Amplification Polymorphism) method revealed clonal variability in the genomes of flatworm Himasthla elongata (Trematoda, Echinostomatidae) larvae. The larvae are parthenogenetic and have been considered genetically homogeneous. We performed cloning and sequencing of an about 500-bp conservative fragment (B1). B1 sequence analysis showed that this fragment had maximal homology with LINE elements from the CR1 family of Hydra and sparrow. In situ hybridization (FISH) revealed B1 dispersed distribution. Several other fragments cloned from the same agarose electrophoresis band correspond to the conservative domain of reverse transcriptase (RT) from CR1 family. Thus, it has been shown that (1) cercariae of trematode H. elongata have clonal variability, (2) the S-SAP method allows patterns to be obtained of fragment distribution characteristic for individual cercariae, and (3) RT conservative domain of the CR1 family participates in the pattern of polymorphic fragments generation. Identification of the CR1 transcripts in H. elongata cercariae transcriptom will be the aim of a future work. Cloning of variable fragments from the pattern of fragments is in progress.
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Abbreviations
- bp:
-
base pair
- AFLP:
-
amplified fragment length polymorphism
- CR1:
-
chicken repeat 1
- CTAB:
-
cetyltrimeth-ylammonium bromide
- LINE:
-
long interspersed nuclear elements
References
Altschul, S.F., Gish, W., Miller, W., Myers, E.W., and Lipman, D.J., Basic local alignment search tool, J. Mol. Biol., 1990, vol. 5, pp. 403–410.
Arkhipova, I. and Meselson, M., Transposable elements in sexual and ancient asexual taxa, Proc. Natl. Acad. Sci. USA, 2000, vol. 97, pp. 14473–14477.
Bao, W. and Jurka, J., CR1 families from Hydra magnipapillata, Repbase Reports, 2008, vol. 8, pp. 1850–1850.
Behura, S., Molecular marker systems in insects: current trends and future avenues, Mol. Ecol., 2006, vol. 15, pp. 3087–3113.
Behura, S., Nair, S., and Mohan, M., Polymorphisms flanking the mariner integration sites in the rice gall midge (Orseolia oryzae Wood-Mason) genome are biotype-specific, Genome, 2001, vol. 44, pp. 947–954.
Birstein, V.J. and Mikhailova, N.A., On the karyology of trematodes of the genus Microphallus and Theirintermediate gastropod host, Littorina saxatilis. I. Chromosome analysis of three Microphallus species, Genetica, 1990, vol. 80, pp. 159–165.
Botros, S., William, S., Ebeid, F., Cioli, D., Katz, N., Day, T., and Bennett, J., Lack of evidence for an antischistosomal activity of myrrh in experimental animals, Am. Soc. Trop. Med. Hyg., 2004, vol. 72, pp. 119–123.
Capy, P., Evolutionary biology. A plastic genome, Nature, 1998, vol. 396, pp. 522–523.
Drew, A.C. and Brindley, P.J., A retrotransposon of the non-long terminal repeat class from the human blood fluke Schistosoma mansoni. Similarities to the chicken-repeat-1-like elements of vertebrates, Mol. Biol. Evol., 1997, vol. 14, pp. 602–610.
Galaktionov, K.V. and Dobrovolskiy, A.A., The biology and evolution of trematodes, in An Essay on the Biology, Morphology, Lifecycles, Transmissions, and Evolution of Digenetic Trematodes, The Netherlands: Kluwer Academic Publisher, 2003.
Galaktionov, N.K., Fedorov, A.V., Galaktionov, K.V., and Podgornaya, O.I., Analysis of inter- and intraclonal genomic diversity of Himasthla elongate (Trematoda; Echinostomatidae) cercariae by AFLP, Parasitol. Res. 2013 (in press).
Galaktionov, N.K., Podgornaya, O.I., and Fedorov, A.V., Characterization of mariner transposon from the genome of Himasthla elongate fluke, Cell Tissue Biol., 2009, vol. 3, no. 6, pp. 526–531.
Giordano, J. Ge, Y., Gelfand, Y., Abrusa’n, G., Benson, G., and Warburton, P.E., Evolutionary history of mammalian transposons determined by genome-wide defragmentation, PLoS Comput Biol., 2007, vol. 3, p. e137.
Grevelding, C., Genomic instability in Schistosoma mansoni, Mol. Biochem. Parasitol., 1999, vol. 101, pp. 207–216.
Hall, T.A., BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT, Nucleic Acids Symp. Ser., 1999, vol. 41, pp. 95–98.
Hass, N.B., Grabowski, J.M, Silvitz, A.B, and Burch, J.B., Chicken Repeat 1 (CR1) Elements, which define an ancient family of vertebrate non-LTR retrotransposons, contain two closely spaced open reading frames, Gene, 1997, vol. 197, pp. 305–309.
Hirai, H. and Hirai, Y., FISH mapping for helminth genomes, Methods Mol. Biol., 2004, vol. 270, pp. 379–394.
Kalendar, R., Lee, D., and Schulman, A., FastPCR software for PCR primer and probe design and repeat search, Genes, Genomes Genomics, 2009, vol. 3, pp. 1–14.
Khalturin, K.V., Mikhaylova, N.I., and Granovich, A.I., Genetic heterogeneity of natural populations of parthenites Microphallus piriformes and M. pygmaeus (Trematoda: Microphallidae), Parazitologiya, 2000, vol. 34, no. 6, pp. 486–500.
Kohany, O., Gentles, A.J., Hankus, L, and Jurka, J., Annotation, submission and screening of repetitive elements in Repbase: Repbase submitter and censor, BMC Bioinform., 2006, vol. 7, p. 474.
Kuznetsova, I.S., Voronin, A.P., and Podgornaya, O.I., Telomere and Trf2/mtbp localization in respect to satellite DNA during the cell cycle of mouse cell line L929, Rejuvenat. Res., 2006, vol. 9, pp. 391–401.
Laha, T., Kewgrai, N., Loukas, A., and Brindley, J.P., Characterization of SR3 reveals abundance of non-LTR retrotransposons of the RTE clade in the genome of the human blood fluke, Schistosoma mansoni, BMC Genom., 2005, vol. 6, p. 154.
Lander, E., Linton, L.M., Birren, B., Nusbaum, C., Zody, M., et al., Initial sequencing and analysis of the human genome, Nature, 2001, vol. 409, pp. 860–921.
Manuelidis, L., A view of interphase chromosomes, Science, 1990, vol. 250, pp. 1533–1540.
Mutafova, T., Kanev, I., and Eizenhut, U., Karyological studies of Isthmiophora melis (Schrank, 1788) from its type locality, J. Helminthol., 1991, vol. 65, pp. 255–258.
Mutafova, T., Karyological studies on some species of the families Echinostomatidae and Plagiorchiidae and aspects of chromosome evolution in trematodes, Syst. Parasitol., 1994, vol. 28, pp. 229–238.
Queen, R.A., Gribbon, B.M., C.James, P., Jack, A., and Flavell, J., Retrotransposon-based molecular markers for linkage and genetic diversity analysis in wheat, Mol. Gen. Genom., 2004, vol. 271, pp. 91–97.
Richard, J. and Voltz, A., Preliminary data on the chromosomes of Echinostoma caproni Richard, 1964 (Trematoda: Echinostomatidae), Syst. Parasitol., 1987, vol. 9, pp. 169–172.
Sambrook, J., Fritsch, E., and Manniatis, T., Molecular Cloning: A Laboratory Manual, 2nd ed., Cold Spring Harbor Laboratory Press, 1989.
Silva, R. and Burch, J.B.E., Evidence that chicken CR1 elements represent a novel family of retroposons, Mol. Cell. Biol., 1989, vol. 9, pp. 3563–3566.
Semyenova, S.K., Chrisanfova, G.G., Fillipova, E.K., Beer, S.A., Voronin, M.V., and Ryskov, A.P., Individual and population variation in cercariae of bird schistosomes of the Trichobilharzia ocellate species group as revealed with the polymerase chain reaction, Russ. J. Genet., 2005, vol. 41, no. 1, pp. 12–16.
Smit, A.F., CR1-X1_Pass—CR1 Non-LTR retrotransposon from Passeriformes, Repbase Reports, 2009, vol. 9, p. 50.
Solovei, I, Kreysing, M., Lanctöt, C., Kösem, S., Peichl, L., Cremer, T., Guck, J., and Joffe, B., Nuclear architecture of rod photoreceptor cells adapts to vision in mammalian evolution, Cell, 2009, vol. 137, pp. 356–368.
Staginnus, C., Desel, C., Schmidt, T., and Kahl, G., Assembling a puzzle of dispersed retrotransposable sequences in the genome of chickpea (Cicer arietinum L.), Genome, 2010, vol. 53, pp. 1090–1102.
Stocking, C. and Kozak, C.A., Murine endogenous retroviruses, Cell. Mol. Life Sci., 2008, vol. 65, pp. 3383–3398.
Venter, J.C., Adams, M.D., Myers, E.W., Li, P.W., Mural, R.J., et al., The sequence of the human genome, Science, 2001, vol. 291, pp. 1304–1351.
Vincze, T., Posfai, J., and Roberts, R., NEBcutter: a program to cleave DNA with restriction enzymes, Nucleic Acids Res., 2003, vol. 31, pp. 3688–3691.
Vos, P., Hogers, R., Bleeker, M., Reijans, M., van de Lee, T., Hornes, M., Frijters, A., Pot, J., Peleman, J., Kuiper, M., and Zabeau, M., AFLP: a new technique for DNA fingerprinting, Nucleic Acids Res., 1995, vol. 23, pp. 4407–4414.
Waugh, R., McLean, K., and Flavell, A., Genetic distribution of Bare-1-like retrotransposable elements in the barley genome revealed by sequence-specific amplification polymorphisms (S-SAP), Mol. Gen. Genet., 1997, vol., 253, pp. 687–694.
Winnepenninckx, B., Backeljau, T., and De, Wachter, R., Extraction of high molecular weight DNA from molluscs, Trends Genet., 1993, vol. 9, pp. 407.
Xiong, Y. and Eickbush, T.H., Origin and evolution of retroelements based on their reverse transcriptase sequences, EMBO J., 1990, vol. 9, pp. 3353–3362.
Zampicinini, G., Blinov, A., Cervella, P., Guryev, V., and Sella, G., Insertional polymorphism of a non-LTR mobile element (NLRCth1) in European populations of Chironomus riparius (Diptera, Chironomidae) as detected by transposon insertion display, Genome, 2004, vol. 47, pp. 1154–1163.
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Original Russian Text © A.I. Solovyeva, N.K. Galaktionov, O.I. Podgornaya, 2013, published in Tsitologiya, 2013, Vol. 55, No. 7, pp. 492–500.
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Solovyeva, A.I., Galaktionov, N.K. & Podgornaya, O.I. LINE class retroposon is a component of DNA polymorphic fragments of trematode Himasthla elongata parthenitae. Cell Tiss. Biol. 7, 563–572 (2013). https://doi.org/10.1134/S1990519X13060126
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DOI: https://doi.org/10.1134/S1990519X13060126