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Immunomodulating effect of cyclophosphamide on cytotoxic activity of rat and mouse splenocytes

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Abstract

The goal of this work consisted in study of the immunomodulating action of the cytostatic drug cyclophosphamide (CP) on the natural cytotoxic activity of rat and mice splenocytes. The cytotoxicity of effector cells (EC) with respect to monolayer cell lines of the Zajdela rat hepatoma and the HTC rat hepatoma and of the MH-22a mouse hepatoma was determined with the aid of morphometric analysis. CP at a dose of 100 mg/kg 48 h after administration to animals has been shown to produce an immunomodulating effect on cytotoxicity of splenocytes—a suppressive one with respect to cell-targets (CT) of Zajdela hepatoma and an immunopotentiating one with respect to CT of HTC and MH-22 hepatomas. Possible mechanisms of the CP immunopotentiating action are discussed.

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Abbreviations

CP:

cyclophosphamide

CT:

cell-targets

BFBS:

bovine fetal blood serum

EC:

effector cells

NK cells:

natural killer cells

PF:

paraformaldehyde

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Authors and Affiliations

  1. Institute of Cytology, Russian Academy of Sciences, St. Petersburg, Russia

    N. P. Teryukova, O. N. Pogodina, G. I. Blinova & V. A. Ivanov

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  1. N. P. Teryukova
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  2. O. N. Pogodina
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  3. G. I. Blinova
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  4. V. A. Ivanov
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Correspondence to N. P. Teryukova.

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Teryukova, N.P., Pogodina, O.N., Blinova, G.I. et al. Immunomodulating effect of cyclophosphamide on cytotoxic activity of rat and mouse splenocytes. Cell Tiss. Biol. 5, 586–594 (2011). https://doi.org/10.1134/S1990519X11060137

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  • DOI: https://doi.org/10.1134/S1990519X11060137

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