Abstract
The structure of a new pelomyxa species was investigated on the level fo light and electron microscopy. The length of locomotive forms of Pelomyxa stagnalis reaches 800 μm. The thin layer of amorphous glycocalyx is located on the cell surface. Numerous nonfunctioning flagellae are revealed predominantly in the uroidal zone. The axoneme has a nonstable set of microtubules. No additional structures are present in the transition zone. The length of P. stagnalis flagella kinetosomes does not exceed 150 nm. Fifteen to twenty microtubules extend from the side surface of each kinetosome at a small angle to the cell surface. One of main components of the P. stagnalis cytoplasm are structural vacuoles. Glycogen bodies in cells are surrounded by flattened ER cisterns, which are often filled with electron-dense material. Cells of P. stagnalis were found to contain two species of prokaryote endobionts that differ in the peculiarities of their fine structure. The number of nuclei in cells of the P. stagnalis adult individuals can reach 50 or more. The nuclei are surrounded by a bilayer envelope formed by the multilaminar layer and by the outer layer composed of vesicles often filled with an electron-dense material. The nucleolus is usually single and is located in the center of the nucleus. In nuclei, predominantly in connection with nucleoli, bodies are formed that are formed by interlacing electron-dense strands.
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Original Russian Text © L.V. Chistyakova, A.O. Frolov, 2010, published in Tsitologiya, vol. 52, No. 12, 2010, pp. 451–458.
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Chistyakova, L.V., Frolov, A.O. Light and electron microscopic study of Pelomyxa stagnalis sp. n. (Archamoebae, pelobiontida). Cell Tiss. Biol. 5, 90–97 (2011). https://doi.org/10.1134/S1990519X11010044
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DOI: https://doi.org/10.1134/S1990519X11010044