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Viability and angiogenic activity of mesenchymal stromal cells from adipose tissue and bone marrow under hypoxia and inflammation in vitro

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Abstract

Progenitor stromal cells derived from adipose tissue (ADSC) and bone marrow (BMDSC) hold great promise for use in the cell-based therapy of ischemic diseases. It was demonstrated that these cells secrete a number of angiogenic cytokines that stimulate vascularization. It was demonstrated that ADSC or BMDSC injected intramuscularly or intravenously into the animals with experimental hind-limb ischemia improve vascularization. However, low oxygen levels and inflammation may impair the viability and functional activity of transplanted cells. We have examined ADSC and BMDSC properties in vitro under hypoxic and inflammatory conditions. ADSC and BMDSC derived from Balb/c mice have been cultivated under hypoxia or in the presence of inflammatory cytokines. The viability of cells assessed by annexin V-PE binding and 7AAD storage, as well as by the quantitative TUNEL method, was not changed under hypoxic conditions Cell exposure to inflammatory cytokines induced apoptosis in 70% of cells. Inflammatory cytokines did not stimulate gene expression of angiogenic growth factors. Under hypoxia conditions up-regulation of genes for pro-angiogenic factors and down-regulation of anti-angiogenic genes were more apparent in ADSC. Using angiogenesis models in vitro and in vivo, we demonstrated that stromal cell maintenance under hypoxic conditions increased their ability to stimulate the growth of blood vessels.

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Authors and Affiliations

  1. Lomonosov Moscow State University, Moscow, Russia

    A. Yu. Efimenko, E. E. Starostina, K. A. Rubina, N. I. Kalinina & E. V. Parfenova

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  1. A. Yu. Efimenko
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  2. E. E. Starostina
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  3. K. A. Rubina
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  4. N. I. Kalinina
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  5. E. V. Parfenova
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Correspondence to A. Yu. Efimenko.

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Original Russian Text © A.Yu. Efimenko, E.E. Starostina, K.A. Rubina, N.I. Kalinina, E.V. Parfenova, 2010, published in Tsitologiya, Vol. 52, No. 2, 2010, pp. 144–154.

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Efimenko, A.Y., Starostina, E.E., Rubina, K.A. et al. Viability and angiogenic activity of mesenchymal stromal cells from adipose tissue and bone marrow under hypoxia and inflammation in vitro. Cell Tiss. Biol. 4, 117–127 (2010). https://doi.org/10.1134/S1990519X1002001X

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  • DOI: https://doi.org/10.1134/S1990519X1002001X

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