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Prenatal Hypoxia Causes an Increase in the Content and Transcriptional Activity of the Hypoxia-Inducible Factor HIF1α in the Hippocampus of Adult and Aging Rats

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Abstract—

Using Western blotting, we conducted a study investigating the expression levels of the regulatory alpha subunit of hypoxia-inducible factor 1 (HIF1α) in the hippocampus of adult and aging rats, whose mothers were exposed to severe damaging hypoxia during days 14-16 of pregnancy. Furthermore, we assessed the relative abundance of mRNA for HIF1-regulated genes (hk1, ldha, mct4, pdk1) using quantitative real-time PCR in adult rats. Our findings indicate that prenatal hypoxia induces a sustained increase in HIF1α expression in the hippocampus. Additionally, we observed an accompanying elevation in the mRNA levels of the investigated HIF1-regulated genes. These results suggest that prenatal hypoxia exerts a lasting impact on hippocampal metabolism. Consequently, these metabolic disruptions may persist throughout life.

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Funding

This study was supported by the Ministry of Education and Science of the Russian Federation, agreement no. 075-15-2020-921 from November 11, 2020 within the project “Integrative Physiology for Medicine, High-Tech Healthcare, and Technologies of Stress Resistance,” direction “The Biological and Social Basis of Inclusiveness.”

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Authors and Affiliations

  1. Pavlov Institute of Physiology, Russian Academy of Sciences, St. Petersburg, Russia

    S. S. Potapova, T. G. Zachepilo, V. A. Stratilov, E. I. Tyulkova & O. V. Vetrovoy

  2. St. Petersburg State University, St. Petersburg, Russia

    S. S. Potapova & O. V. Vetrovoy

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  1. S. S. Potapova
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  2. T. G. Zachepilo
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  3. V. A. Stratilov
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  4. E. I. Tyulkova
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  5. O. V. Vetrovoy
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Corresponding author

Correspondence to O. V. Vetrovoy.

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ETHICS APPROVAL AND CONSENT TO PARTICIPATE

All the experiments were performed according to the rules of the European Council Directive 86/609/EEC on the use of animals for experimental studies. The protocols of the experiments were approved by the Commission for the Humane Treatment of Animals of the Pavlov Institute of Physiology, Russian Academy of Sciences.

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The authors of this work declare that they have no conflicts of interest.

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Corresponding author; address: 199034 Russia, St. Petersburg, nab. Makarova, 6, e-mail: vov210292@yandex.ru.

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Potapova, S.S., Zachepilo, T.G., Stratilov, V.A. et al. Prenatal Hypoxia Causes an Increase in the Content and Transcriptional Activity of the Hypoxia-Inducible Factor HIF1α in the Hippocampus of Adult and Aging Rats. Neurochem. J. 17, 751–754 (2023). https://doi.org/10.1134/S1819712423330012

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  • DOI: https://doi.org/10.1134/S1819712423330012

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