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Superoxide Dismutase and Clopidogrel: A Potential Role in Peripheral Arterial Disease Treatment

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Abstract

Oxidative stress plays a crucial role in the pathogenesis of peripheral artery disease (PAD). This study aimed to investigate the effect of clopidogrel on oxidative stress in PAD patients. Seventy subjects were divided into three groups: PAD patients before treatment (B-PAD), PAD patients after treatment with clopidogrel (A-PAD), and healthy controls. Serum levels of superoxide dismutase (SOD), copper (Cu), zinc (Zn), manganese (Mn), and oxidized protein were measured. SOD activities were also determined. The results showed that SOD activities, and SOD specific activities were significantly decreased in PAD patients compared to healthy individuals. After treatment with clopidogrel, SOD activities, and SOD specific activities were continuously decrease in PAD patients. The SOD and oxidized protein concentrations were significantly increased in PAD patients compared to healthy individuals. After treatment with clopidogrel, the oxidized protein concentration was significantly decreased, while SOD concentration was significantly increased in PAD patients. These findings suggest that the treatment by clopidogrel stimulated the production of the enzyme but the ratio of active enzyme remained low. The decrease in oxidized protein can be explained by the treatment having antioxidant efficacy that may have compensated for the deficiency in enzyme activity and led to a decrease in oxidized protein. Additionally, the results of this study provide promising evidence that oxidative stress biomarkers including SOD concentration, T-SOD activity, Mn-SOD activity, and oxidized protein levels have potential utility in the diagnosis and management of PAD.

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ACKNOWLEDGMENTS

The authors are grateful to the University of Baghdad, College of Science for supporting this manuscript.

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This work was supported by ongoing institutional funding. No additional grants to carry out or direct this particular research were obtained.

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  1. Department of Chemistry, College of Science, University of Baghdad, 10071, Baghdad, Iraq

    Yasameen Ali Fakhri & Ali W. Al-Ani

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This work does not contain any studies involving human and animal subjects. Data analysis was performed from the open-access database Pulse Transit Time PPG Dataset (version 1.1.0) [9]. According to the Pulse Transit Time PPG Dataset data, all studies were conducted in accordance with the principles of biomedical ethics set out in the 1964 Declaration of Helsinki and its subsequent amendments, and all participants provided written informed consent.

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Fakhri, Y.A., Al-Ani, A.W. Superoxide Dismutase and Clopidogrel: A Potential Role in Peripheral Arterial Disease Treatment. Dokl Biochem Biophys (2024). https://doi.org/10.1134/S1607672924600088

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