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The Protection of Enzyme Activity for the Preparation of Humanized Polymerized Hemoglobin-Superoxide Dismutase-Catalase-Carbonic Anhydrase

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Abstract

This work finds suitable enzyme activity protectants to improve the recovery rate of enzyme activity in the preparation of human polymerized hemoglobin-superoxide dismutase-catalase-carbonic anhydrase (PolyHb-SOD-CAT-CA), including trehalose, sucrose, glucose, hydroxypropyl-β-cyclodextrin, and mannitol.Different types and concentrations of enzyme activity protective agents were added during polymerization to compare their protective ability to enzyme activity and the effect on the properties of hemoglobin. The study found that compared with trehalose, the protective effect of sucrose on CA enzyme activity is non-significant to that on hemoglobin, the recovery rate of SOD, and CAT enzyme activity has significant increased. Glucose, hydroxypropyl-β-cyclodextrin, and mannitol are unsuitable for the added enzyme activity protective agent of PolyHb-SOD-CAT-CA.The protective effect of sucrose on CA was non-significant with trehalose. The protective effect of sucrose on SOD and CAT enzyme activity was higher than trehalose, and the protective effect reached the maximum when the concentration reached 1.5%.

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Funding

The authors are grateful to the financial support from the Science and Technology Platform Talents Project of Guizhou Province (Qiankehe Platform Talents (2018) 5772-024).

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Authors and Affiliations

  1. College of Life Science and Engineering, Southwest Jiaotong University, Chengdu, Sichuan, PR China

    Yaoxi Wang & Wanjun Wang

  2. Department of Bioengineering, Zhuhai Campus of Zunyi Medical University, Zhuhai City, Guangdong Province, PR China

    Lili Zhang & Gang Chen

  3. Beijing Pro-heme Biotech Co.ltd, Beijing, Changping District, PR China

    Gang Chen

Authors
  1. Yaoxi Wang
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  2. Wanjun Wang
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  3. Lili Zhang
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  4. Gang Chen
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Contributions

Yaoxi Wang: Data curation, Investigation, Wanjun Wang: Methodology, Lili Zhang: Writing, Gang Chen: Conceptualization.

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Correspondence to Gang Chen.

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The authors of this work declare that they have no conflicts of interest.

ETHICS APPROVAL AND CONSENT TO PARTICIPATE

All studies were conducted in accordance with the principles of biomedical ethics as outlined in the 1964 Declaration of Helsinki and its later amendments. They were also approved by the Ethics Committee of Zunyi Medical University (code: 20220602Z15, date: June 3, 2022). Each participant in the study provided a voluntary written informed consent after receiving an explanation of the potential risks and benefits, as well as the nature of the upcoming study.

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Wang, Y., Wang, W., Zhang, L. et al. The Protection of Enzyme Activity for the Preparation of Humanized Polymerized Hemoglobin-Superoxide Dismutase-Catalase-Carbonic Anhydrase. Dokl Biochem Biophys (2024). https://doi.org/10.1134/S1607672923600483

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  • DOI: https://doi.org/10.1134/S1607672923600483

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