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The First Recombinant Viper Three-Finger Toxins: Inhibition of Muscle and Neuronal Nicotinic Acetylcholine Receptors

Doklady Biochemistry and Biophysics volume 479, pages 127–130 (2018)Cite this article

Abstract

Genes encoding two three-finger toxins TFT-AF and TFT-VN, nucleotide sequences of which were earlier determined by cloning cDNA from venom glands of vipers Azemiops feae and Vipera nikolskii, respectively, were expressed for the first time in E. coli cells. The biological activity of these toxins was studied by electrophysiological techniques, calcium imaging, and radioligand analysis. It was shown for the first time that viper three-finger toxins are antagonists of nicotinic acetylcholine receptors of neuronal and muscle type.

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Authors and Affiliations

  1. Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russia

    Ya. V. Makarova, E. V. Kryukova, I. V. Shelukhina, D. S. Lebedev, T. V. Andreeva, D. Yu. Ryazantsev, S. V. Balandin, T. V. Ovchinnikova, V. I. Tsetlin & Yu. N. Utkin

  2. Sechenov First Moscow State Medical University, Ministry of Healthcare of the Russian Federation, Moscow, 119992, Russia

    T. V. Ovchinnikova

Authors
  1. Ya. V. Makarova
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  2. E. V. Kryukova
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  3. I. V. Shelukhina
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  4. D. S. Lebedev
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  5. T. V. Andreeva
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  6. D. Yu. Ryazantsev
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  7. S. V. Balandin
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  8. T. V. Ovchinnikova
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  9. V. I. Tsetlin
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  10. Yu. N. Utkin
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Correspondence to Ya. V. Makarova.

Additional information

Original Russian Text © Ya.V. Makarova, E.V. Kryukova, I.V. Shelukhina, D.S. Lebedev, T.V. Andreeva, D.Yu. Ryazantsev, S.V. Balandin, T.V. Ovchinnikova, V.I. Tsetlin, Yu.N. Utkin, 2018, published in Doklady Akademii Nauk, 2018, Vol. 479, No. 6, pp. 708–711.

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Makarova, Y.V., Kryukova, E.V., Shelukhina, I.V. et al. The First Recombinant Viper Three-Finger Toxins: Inhibition of Muscle and Neuronal Nicotinic Acetylcholine Receptors. Dokl Biochem Biophys 479, 127–130 (2018). https://doi.org/10.1134/S1607672918020205

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  • DOI: https://doi.org/10.1134/S1607672918020205