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Studying a novel ecdysone-dependent enhancer

  • Biochemistry, Biophysics, and Molecular Biology
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Abstract

Earlier [4], we developed a two-stage scheme of the activation of expression of the ecdysone cascade gene ftz-f1 in Drosophila melanogaster S2 cells. In the first intron of the ftz-f1 gene, we found a binding site for the transcription activator DHR3. In this work, we studied the properties of this genomic element: the change in the histone modification level at different stages of transcription activation were analyzed, and data indicating the interaction of the intron element with the promoter were obtained. Taken together, the results of this study indicate that the studied genomic element exhibits the properties of an enhancer and functions at the stage of active gene transcription.

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Authors and Affiliations

  1. Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334, Russia

    J. V. Nikolenko, A. N. Krasnov, M. Y. Mazina, S. G. Georgieva & N. E. Vorobyeva

  2. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia

    S. G. Georgieva

Authors
  1. J. V. Nikolenko
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  2. A. N. Krasnov
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  3. M. Y. Mazina
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  4. S. G. Georgieva
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  5. N. E. Vorobyeva
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Corresponding author

Correspondence to J. V. Nikolenko.

Additional information

Original Russian Text © J.V. Nikolenko, A.N. Krasnov, M.Y. Mazina, S.G. Georgieva, N.E. Vorobyeva, 2017, published in Doklady Akademii Nauk, 2017, Vol. 474, No. 6, pp. 756–759.

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Nikolenko, J.V., Krasnov, A.N., Mazina, M.Y. et al. Studying a novel ecdysone-dependent enhancer. Dokl Biochem Biophys 474, 236–238 (2017). https://doi.org/10.1134/S160767291703022X

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  • DOI: https://doi.org/10.1134/S160767291703022X

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