Skip to main content
SpringerLink
Log in

A flow-through enzymatic bioreactor based on immobilized α-chymotrypsin

  • Macromolecular Chemistry and Polymeric Materials
  • Published:
Russian Journal of Applied Chemistry Aims and scope Submit manuscript

Abstract

With the aim to develop a flow-through enzymatic bioreactor based on a macroporous monolithic sorbent, immobilization of chymotrypsin was performed by the reaction of amino groups of the enzyme with epoxy groups of the sorbent. The enzymatic hydrolysis of low- and high-molecular-weight substrates, N-benzoyl-L-tyrosine ethyl ester and human serum albumin, was studied.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Tennikova, T.B., Bleha, M., Svec, F., et al., J. Chromatogr., 1991, vol. 555, pp. 97–107.

    Article  CAS  Google Scholar 

  2. Tennikova, T.B. and Svec, F., J. Chromatogr., 1993, vol. 646, pp. 279–288.

    Article  CAS  Google Scholar 

  3. Ostryanina, N.D., Il’ina, O.V., and Tennikova, T.B., J. Chromatogr. B, 2002, vol. 770, pp. 35–43.

    Article  CAS  Google Scholar 

  4. Monolithic Materials: Preparation, Properties and Applications, Svec, F., Tennikova, T., and Dayl, Z., Eds., Amsterdam: Elsevier, 2003.

    Google Scholar 

  5. Vlakh, E.G. and Tennikova, T.B., J. Sep. Sci., 2007, vol. 30, pp. 2801–2813.

    Article  CAS  Google Scholar 

  6. Vlakh, E.G. and Tennikova, T.B., J. Chromatogr. A, 2009, vol. 1216, pp. 2637–2650.

    Article  CAS  Google Scholar 

  7. Hilder, E.F., Svec, F., and Frechet, J.M.J., J. Chromatogr. A, 2004, vol. 1044, pp. 3–22.

    Article  CAS  Google Scholar 

  8. Svec, F. and Kurganov, A., J. Chromatogr. A, 2008, vol. 1184, pp. 281–295.

    Article  CAS  Google Scholar 

  9. Tripp, J.A., Stein, J.A., Svec, F., et al., Org. Lett., 2000, vol. 2, pp. 195–198.

    Article  CAS  Google Scholar 

  10. Petro, M., Svec, F., and Frechet, J.M.J., Biotechnol. Bioeng., 1996, vol. 49, pp. 355–363.

    Article  CAS  Google Scholar 

  11. Platonova, G.A., Surzhik, M.A., Tennikova, T.B., et al., Bioorg. Khim., 2000, vol. 25, no. 3, pp. 189–194.

    Google Scholar 

  12. Bencina, M., Babic, J., and Podgornik, A., J. Chromatogr. A, 2007, vol. 1144, pp. 135–142.

    Article  CAS  Google Scholar 

  13. Dixon, M. and Webb, E.C., Enzymes, New York: Academic, 1979, 3rd ed.

    Google Scholar 

  14. Williams, K.R. and Stone, K.L., Mol. Biotechnol., 1997, vol. 8, pp. 155–167.

    Article  CAS  Google Scholar 

  15. Rodrigues, A.E., Lopez, J.C., Lu, Z.P., et al., J. Chromatogr., 1992, vol. 590, pp. 93–100.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Macromolecular Compounds, Russian Academy of Sciences, St. Petersburg, Russia

    G. A. Platonova, E. G. Vlakh, N. D. Ivanova & T. B. Tennikova

Authors
  1. G. A. Platonova
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E. G. Vlakh
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. N. D. Ivanova
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. T. B. Tennikova
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Original Russian Text © G.A. Platonova, E.G. Vlakh, N.D. Ivanova, T.B. Tennikova, 2009, published in Zhurnal Prikladnoi Khimii, 2009, Vol. 82, No. 12, pp. 2038–2042.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Platonova, G.A., Vlakh, E.G., Ivanova, N.D. et al. A flow-through enzymatic bioreactor based on immobilized α-chymotrypsin. Russ J Appl Chem 82, 2182–2186 (2009). https://doi.org/10.1134/S1070427209120179

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1070427209120179

Keywords

Search

Navigation