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Investigation of restriction endonuclease EcoRII complex with DNA in solution by FTIR spectroscopy

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Abstract

The X-ray structure of type IIE EcoRII restriction endonuclease has been solved but the structure of the R.EcoRII-DNA complex is still unknown. We report here on the structure of the pre-reactive R.EcoRII-DNA-Ca2+ complex in solution examined by FTIR spectroscopy. The secondary structure of R.EcoRII as well as the structure of the target DNA in the R.EcoRII-DNA-Ca2+ complex was characterized. It was shown that the R.EcoRII-DNA-Ca2+ complex formation is accompanied by changes in the spectrum of both DNA bases and DNA sugar-phosphate backbone that suggest contacts of the enzyme with different groups of atoms in DNA. The change of the R.EcoRII secondary structure in the R.EcoRII-DNA-Ca2+ complex is also observed.

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References

  1. Pingoud, A., Fuxreiter, M., Pingoud, V., and Wende, W., Cell. Mol. Life Sci., 2005, vol. 62, p. 685.

    Article  CAS  Google Scholar 

  2. Roberts, R.J. and Halford, S.E., Type II restricton endonucleases, in Nucleases, Linn, S.M., Lloyd, R.S., and Roberts, R.J., Eds., New York: Cold Spring Harbor Laboratory Press, 1993, 2nd ed.

    Google Scholar 

  3. Roberts, R.J., Belfort, M., Bestor, T., et al., Nucl. Acids Res., 2003, vol. 31, p. 1805.

    Article  CAS  Google Scholar 

  4. Mucke, M., Kruger, D.H., and Reuter, M., Ibid., 2003, vol. 31, p. 6079.

    Article  CAS  Google Scholar 

  5. Halford, S.E., Welsh, A.J., and Szczelkun, M.D., Annu. Rev. Biophys. Biomol. Struct., 2004, vol. 33, p. 1.

    Article  CAS  Google Scholar 

  6. Kirsanova, O.V., Baskunov, V.B., and Gromova, E.S., Mol. Biol. (Mosk.), 2004, vol. 38, p. 886.

    CAS  Google Scholar 

  7. Oller, A.R., Vanden Broek, W., Conrad, M., and Topal M.D., Biochemistry, 1991, vol. 30, p. 2543.

    Article  CAS  Google Scholar 

  8. Jo, K. and Topal, M.D., Science, 1995, vol. 267, p. 1817.

    Article  CAS  Google Scholar 

  9. Huai, Q., Colandene, J.D., Chen, Y., Luo, F., Zhao, Y., Topal, M.D., and Ke, H., EMBO J., 2000, vol. 19, p. 3110.

    Article  CAS  Google Scholar 

  10. Kruger, D.H., Barcak, G.J., Reuter, M., and Smith, H.O., Nucl. Acids Res., 1988, vol. 16, p. 3997.

    Article  CAS  Google Scholar 

  11. Pein, C.D., Reuter, M., Cech, D., and Kruger, D.H., FEBS Lett., 1989, vol. 245, p. 141.

    Article  CAS  Google Scholar 

  12. Reuter, M., Kupper, D., Meisel, A., Schroeder, C., and Kruger, D.H., J. Biol. Chem., 1998, vol. 273, p. 8294.

    Article  CAS  Google Scholar 

  13. Mucke, M., Lurz, R., Mackeldanz, P., Behlke, J., Kruger, D.H., and Reuter, M., Ibid., 2000, vol. 275, p. 30 631.

  14. Petrauskene, O.V., Karpova, E.A., Gromova, E.S., and Guschlbauer, W., Biochem. Biophys. Res. Commun., 1984, vol. 198, p. 885.

    Article  Google Scholar 

  15. Tamulaitis, G., Sasnauskas, G., Mucke, M., and Siksnys, V., J. Mol. Biol., 2006, vol. 358, p. 406.

    Article  CAS  Google Scholar 

  16. Zhou, X.E., Wang, Y., Reuter, M., Mucke, M., Kruger, D.H. Meehan, E.J., and Chen, L., Ibid., 2004, vol. 335, p. 307.

    Article  CAS  Google Scholar 

  17. Mucke, M., Grelle, G., Behlke, J., Kraft, R., Kruger, D.H., and Reuter, M., EMBO J., 2002, vol. 21, p. 5262.

    Article  Google Scholar 

  18. Tamulaitis, G., Mucke, M., and Siksnys, V., FEBS Lett., 2006, vol. 580, p. 1665.

    Article  CAS  Google Scholar 

  19. Pingoud, A. and Jeltsch, A., Eur. J. Biochem., 1997, vol. 246, p. 1.

    Article  CAS  Google Scholar 

  20. Kruger, D.H., Kupper, D., Meisel, A., Reuter, M., and Schroeder, C., FEMS Microbiol. Rev., 1995, vol. 17, p. 177.

    Article  CAS  Google Scholar 

  21. Bochtler, M., Szczepanowski, R.H., Tamulaitis, G., Grazulis, S., Czapinska, H., Manakova, E., and Siksnys, V., EMBO J., 2006, vol. 25, p. 2219.

    Article  CAS  Google Scholar 

  22. Surewicz, W.K., Mantsch, H.H., and Chapman, D., Biochemistry, 1993, vol. 32, p. 389.

    Article  CAS  Google Scholar 

  23. Byler, D.M. and Susi, H., Biopolymers, 1986, vol. 25, p. 469.

    Article  CAS  Google Scholar 

  24. Surewicz, W.K. and Mantsch, H.H., Biochim. Biophys. Acta, 1988, vol. 952, p. 115.

    CAS  Google Scholar 

  25. Arrondo, J.L., Muga, A., Castresana, J., and Goni, F.M., Prog. Biophys. Mol. Biol., 1993, vol. 59, p. 23.

    Article  CAS  Google Scholar 

  26. Jackson, M. and Mantsch, H.H., Crit. Rev. Biochem. Mol. Biol., 1995, vol. 30, p. 95.

    Article  CAS  Google Scholar 

  27. Taillandier, E. and Liquer, J., Handbook of Vibrational Spectroscopy, 2002, vol. 5.

  28. Polyanichko, A. and Wieser, H., Biopolymers, 2005, vol. 78, p. 329.

    Article  CAS  Google Scholar 

  29. Taillandier, E., Taboury, J., Liquier, J., Sautiere, P., and Couppez, M., Biochimie, 1981, vol. 63, p. 895.

    Article  CAS  Google Scholar 

  30. Liquier, J., Gadenne, M.C., Taillandier, E., Defer, N., Favatier, F., and Kruh, J., Nucl. Acids Res., 1979, vol. 6, p. 1479.

    Article  CAS  Google Scholar 

  31. Vila, R., Ponte, I., Collado, M., Arrondo, J.L., and Suau, P., J. Biol. Chem., 2001, vol. 276, p. 30 898.

  32. Vila, R., Ponte, I., Collado, M., Arrondo, J.L., Jimenez, M.A., Rico, M., and Suau, P., Ibid., 2001, vol. 276, p. 46 429.

  33. Babkina, O.V., Evstaf’eva, A.G., Chichkova, N.V., et al., Mol. Biol. (Mosk.), 2000, vol. 34, p. 1065.

    CAS  Google Scholar 

  34. Cantor, C.R., Warshaw, M.M., and Shapiro, H., Biopolymers, 1970, vol. 9, p. 1059.

    Article  CAS  Google Scholar 

  35. Subach, F.V., Muller, S., Tashlitsky, V.N., Petrauskene, O.V., and Gromova, E.S., Bioorg. Khim., 2003, vol. 29, p. 623.

    CAS  Google Scholar 

  36. Polyanichko, A.M., Andrushchenko, V.V., Chikhirzhina, E.V., et al., Nucl. Acids Res., 2004, vol. 32, p. 989.

    Article  CAS  Google Scholar 

  37. Gromova, E.S., Kubareva, E.A., Vinogradova, M.N., et al., J. Mol. Recognit., 1991, vol. 4, p. 133.

    Article  CAS  Google Scholar 

  38. Baskunov, V.B., Subach, F.V., Kolbanovskiy, A., et al., Biochemistry, 2005, vol. 44, p. 1054.

    Article  CAS  Google Scholar 

  39. Howard, F.B. and Miles, H.D., J. Biol. Chem., 1965, vol. 240, p. 801.

    CAS  Google Scholar 

  40. Babkina, O.V., Chutko, C.A., Shashkov, A.A., et al., Photochem. Photobiol. Sci., 2002, vol. 1, p. 636.

    Article  CAS  Google Scholar 

  41. Petrauskene, O.V., Yakovleva, J.N., Alekseev, Y.I., et al., J. Biomol. Struct. Dyn., 2000, vol. 17, p. 857.

    CAS  Google Scholar 

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Authors and Affiliations

  1. Albert Einstein Medical College, New York, USA

    F. V. Subach

  2. Department Health, Medicine, and Human Biology, University Paris-13, Paris-13, France

    J. Liquier

  3. Lomonosov Moscow State University, Leninskie gory 1, str. 3, Moscow, 119991, Russia

    E. S. Gromova

Authors
  1. F. V. Subach
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  2. J. Liquier
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  3. E. S. Gromova
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Correspondence to F. V. Subach.

Additional information

Original Russian Text © F.V. Subach, J. Liquier, E.S. Gromova, 2008, published in Rossiiskii Khimicheskii Zhurnal, 2007, Vol. 51, No. 6, pp. 89–93.

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Subach, F.V., Liquier, J. & Gromova, E.S. Investigation of restriction endonuclease EcoRII complex with DNA in solution by FTIR spectroscopy. Russ J Gen Chem 78, 1103–1109 (2008). https://doi.org/10.1134/S1070363208050435

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