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Hybrid Antimicrobial Coating Based on Conjugate of Hyaluronic Acid with LL-37 Peptide for PEO-Modified Titanium Implants

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Abstract

Objective: Synthesis of a novel conjugate of hyaluronic acid (HA) and the antimicrobial peptide LL-37 and evaluation of its antibacterial activity as an organic coating for titanium implants. Methods: The hybrid molecule HA–LL-37 was prepared by the Michael reaction of HA-SH and the N-maleimide derivative of LL-37. The hybrid compound has been tested as an antimicrobial organic coating for titanium samples with an inorganic sub-layer created by plasma electrolytic oxidization (PEO) of the surface. Results and Discussion: The in vitro studies have demonstrated the antibacterial effect of the hybrid molecule within the inorganic PEO coating, i.e., a significant (p <0.05) suppression of the ability of Staphylococcus aureus, Pseudomonas aeruginosa, Enterococcus faecium, and Escherichia coli to form biofilms. Conclusions: The presented approach can be utilized for the subsequent design and development of non-fouling antimicrobial coatings of implants to decrease the risk of bacteria-induced diseases.

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DATA AVAILABILITY

The data that support the findings of this study are available from the corresponding author upon reasonable request.

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ACKNOWLEDGMENTS

Structural studies were performed at the Regional Center for Collective Use “Agidel” of the Ufa Federal Research Center of the Russian Academy of Sciences.

Funding

The work was supported by State Assignment of the Institute of Petrochemistry and Catalysis of the Ufa Federal Research Center of the Russian Academy of Sciences (no. FMRS-2022-0081).

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Authors and Affiliations

  1. Institute of Petrochemistry and Catalysis of Russian Academy of Sciences, 450075, Ufa, Russia

    L. V. Parfenova, Z. R. Galimshina, G. U. Gil’fanova & E. I. Alibaeva

  2. Institute of Cellular and Intracellular Symbiosis, Ural Branch of Russian Academy of Sciences, 460000, Orenburg, Russia

    T. M. Pashkova & O. L. Kartashova

  3. Ufa University of Science and Technology, 450076, Ufa, Russia

    R. G. Farrakhov, V. R. Aubakirova & E. V. Parfenov

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  1. L. V. Parfenova
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  2. Z. R. Galimshina
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  4. E. I. Alibaeva
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Correspondence to L. V. Parfenova.

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Parfenova, L.V., Galimshina, Z.R., Gil’fanova, G.U. et al. Hybrid Antimicrobial Coating Based on Conjugate of Hyaluronic Acid with LL-37 Peptide for PEO-Modified Titanium Implants. Russ J Bioorg Chem 50, 500–507 (2024). https://doi.org/10.1134/S1068162024020225

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