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Investigation of the Effect of Tamoxifen on hERG1 Potassium Channel Gene Expression in MCF-7 Cell Line

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Abstract

Objective: Tamoxifen (TAM) is a nonstreroidal antiestrogenic pharmaceutical agent extensively employed in the therapeutic management of breast cancer. Alterations in the expression patterns of diverse ion channels have been implicated in the pathophysiology of breast cancer. The principal objective of this investigation was to elucidate the impact of TAM on the expression of the hERG1 potassium channel in the MCF-7 cell line. Methods: Cytotoxicity induced by TAM across various concentrations was assessed through the utilization of the MTT method. The patch clamp method was employed for electrophysiological analyses. The gene expression of the hERG1 potassium channel was quantified utilizing RT-qPCR. Results: The electrophysiological findings revealed reduction in potassium channel currents, reaching maximal inhibition at a TAM concentration of 5 μM. Concurrently, hERG1 potassium channel gene expression exhibited a decrease at the 5 μM TAM concentration. Despite statistical analysis, no statistically significant values were identified in both potassium channel currents and gene expression analyses when compared to the control group. Our results demonstrate that the blocking effect of TAM on potassium channel currents in the MCF-7 cell line was not determined. Conclusions: Further investigations are warranted to elucidate comprehensively the role of TAM in modulating hERG1 in breast cancer.

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DATA AVAILABILITY

The data that support the findings of this study are available from the corresponding author upon reasonable request.

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Funding

The authors would like to thank to Mersin University Scientific Research Project Unit for financial support (no. BAP-SBEBK(PE)2011-3DR).

Author information

Authors and Affiliations

  1. Department of Chemistry, Faculty of Science, Mersin University, 33343, Mersin, Turkey

    P. Eroglu

  2. Department of Perfusion Technology, Vocational School of Medical Services, Mersin University, 33343, Mersin, Turkey

    F. Sogut

  3. Institute of Advanced Technology Research and Application, Mersin University, 33343, Mersin, Turkey

    D. Yetkin

  4. Department of Biochemistry, Mersin University, Faculty of Pharmacy, Mersin University, 33169, Mersin, Turkey

    S. Yalin

  5. Department of Biophysics, Faculty of Medicine, Mersin University, 33343, Mersin, Turkey

    U. Comelekoglu

  6. Department of Histology-Embryology, Faculty of Medicine, Mersin University, 33343, Mersin, Turkey

    S. N. Yilmaz

  7. Department of Biostatistics and Medical Informatics, Faculty of Medicine, Mersin University, 33343, Mersin, Turkey

    S. Erdoğan

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  1. P. Eroglu
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Contributions

The authors PE, FS, SY, UC, and NSY designed the experiments. The authors PE, FS, DY, and NSY carried out their biological study. The authors PE, SY, FS, UC, NSY, and SE participated in data processing and contributed to manuscript preparation.

All authors participated in the discussions.

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Correspondence to P. Eroglu.

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This article does not contain any studies involving patients or animals as test objects.

Informed consent was not required for this article. No conflict of interest was declared by the authors

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Eroglu, P., Sogut, F., Yetkin, D. et al. Investigation of the Effect of Tamoxifen on hERG1 Potassium Channel Gene Expression in MCF-7 Cell Line. Russ J Bioorg Chem 50, 392–400 (2024). https://doi.org/10.1134/S106816202402016X

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  • DOI: https://doi.org/10.1134/S106816202402016X

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