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Borneol Induced Hepatotoxicity via Caspase-3 and Oxidative Stress Pathwaysin in Zebrafish

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Abstract

Objective: Borneol is a widely used Chinese medicine, and borneol extracted from plants or synthesized are the two major types of borneol applied in clinical therapy. In this study, the hepatotoxicity of natural and synthetic borneol in both larval and adult zebrafish was detected. Methods: Adult fish and larval were exposed to borneol. Results: Synthetic borneol induced more obvious developmental toxicity, such as morphological deformity, delay of yolk sac absorption, disappearance of swim bladder, loose hepatic tissue, irregular cells, cell vacuolation and decline in cell count; treatment with synthetic borneol caused more severe oxidative stress, as changes in the activities of CAT and SOD and accumulation of ROS and MDA are more notable; synthetic borneol could disrupt lipid and glycogen metabolism more seriously possibly due to the more notable expression changes of metabolismrelated genes (ide, sirt4, dgat1b, and mgst1); synthetic borneol possibly produced hepatic injury by inducing cell apoptosis. Discussion: Our results suggest synthetic borneol has greater potential to cause hepatotoxicity compare to natural borneol. Conclusions: SB showed higher developmental toxicity than NB in larval zebrafish. Exposure to SB disordered the liver’s lipid and glycogen metabolism caused by liver injury in higher concentration groups These findings have implications for understanding the mechanism of the hepatotoxicity caused by borneol, and provide a scientific basis for the drug safety of borneol.

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DATA AVAILABILITY

The data that support the findings of this study are available from the corresponding author upon reasonable request.

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ACKNOWLEDGMENTS

The authors thank for Jun Zhou (Jiangxi Linke Longnao Co., LTD) for providing free the borneol.

Funding

This work was supported by the Key Science and technology Projects of Ji’an city (no. 20211-055455), Jinggangshan University Local Service Project (nos. JFD1804 and JFD1904).

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Authors and Affiliations

  1. College of Life Sciences, Jinggangshan University, Center for Clinical Medicine Research of Jinggangshan University, Ji’an, 343009, Jiangxi, China

    Xinjun Liao, Kun Jia, Lirong Huang & Zigang Cao

  2. Affiliated Hospital of Jinggangshan University, Ji’an, 343009, Jiangxi, China

    Xinjun Liao, Kun Jia, Lirong Huang, Zigang Cao & Huiqiang Lu

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  1. Xinjun Liao
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  2. Kun Jia
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Contributions

The authors XL and KJ writing original draft, methodology, formal analysis, editing, and funding acquisition. The author LH—formal analysis, methodology. The author ZC— validation, methodology. The author HL—validation, methodology, supervision, and funding acquisition.

All authors participated in the discussions.

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Correspondence to Huiqiang Lu.

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Liao, X., Jia, K., Huang, L. et al. Borneol Induced Hepatotoxicity via Caspase-3 and Oxidative Stress Pathwaysin in Zebrafish. Russ J Bioorg Chem 50, 508–521 (2024). https://doi.org/10.1134/S1068162024020080

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