Abstract
Cancer, with the arising rate in developed and developing countries, was the 2nd cause of mortality in 2019; one of the greatest concerns is thus drug resistance in different cancers. Recently, essential oils, have been received more attention for preparing new drugs. In this study, a (Carvacrol-rich essential oil) and Carvacrol were first successfully entrapped in chitosan nanoparticles. A comprehensive comparison was then conducted on cytotoxic effects of non-formulated samples and the prepared nanoformulations on two cancer cell lines (A-375 and MDA-MB-468). The particle sizes of the prepared nanoformulation were 196 ± 8 and 211 ± 13 nm, respectively. The cytotoxic effect of chitosan nanoparticles containing Satureja khuzistanica essential oil (IC50 = 78.8 µg/mL) was more potent than other samples on A-375 cells (human melanoma cancer). However, chitosan nanoparticles containing Carvacrol indicated a more potent anticancer effect than other samples (IC50 = 43.5 µg/mL). As the nanoformulations promising efficacies and green constituents, they are thus good candidates for further research in animal models.
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ACKNOWLEDGMENTS
The authors are thankful to the Fasa University of Medical Sciences.
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Fasa University of Medical Sciences financially supported this research, grant number 401143, Ethics approval and consent to participate: The ethical committee has ethically approved this research; IR.FUMS.REC.1401.127.
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Abbreviations: ChiNPs, chitosan nanoparticles; Eos, essential oils; Satureja khuzistanica, S. khuzistanica; FBS, fetal bovine serum; PBS, phosphate-buffered saline; DMSO, dimethyl sulfoxide; MTT, 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazo-lium bromide; DLS, dynamic light scattering; TPP, sodium-tri-polyphosphate; SKChiNPs, Satureja khuzistanica chitosan nanoparticles; CarChiNPs, carvacrol chitosan nanoparticles.
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Osanloo, M., Alipanah, H., Farjam, M. et al. Anticancer Activity of Chitosan Nanoparticles Containing Satureja khuzistanica Essential Oil, and Carvacrol against Human Melanoma and Breast Cancer. Russ J Bioorg Chem 49, 594–601 (2023). https://doi.org/10.1134/S1068162023030160
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DOI: https://doi.org/10.1134/S1068162023030160