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Identification of SOCS5 Gene in the Chinese Giant Salamander (Andrias davidianus) and Expression Profiles in Response to Citrobacter freundii Challenge

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Abstract—

The full-length cDNA sequences of suppressor of cytokine signaling 5 (SOCS5) from Andrias davidianus gene, named as adSOCS5, was cloned and characterized in the present study. The full-length cDNAs of adSOCS5 with 1908 bp long encoded corresponding proteins of 536 amino acids. Structure analysis indicated that adSOCS5 possessed a conserved Src homology 2 (SH2) domain and a C terminal SOCS box domain. Phylogenetic analysis and 3D structure analysis revealed that adSOCS5 is comparatively similar to SOCS5 of G. evgoodei. Transcripts of adSOCS5 were ubiquitously detected in ten tissues examined but varied among tissues. The highest mRNA expression of adSOCS5 was detected in the ovary and lowest in the muscle. Finally, the ovary and muscle tissue were selected for preliminarily investigating the role of adSOCS5 in immune response. Significant up-regulations of the expressions of adSOCS5 were observed in the ovary and muscle in response to Citrobacter freundii challenge. The adSOCS5 expression in the ovary was more sensitive to C. freundii than the muscle. This work provides valuable information about the characterization of SOCS5 of A. davidianus, which facilitating further research of function of SOCSs in amphibians.

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REFERENCES

  1. Cianciulli, A., Calvello, R., Porro, C., Trotta, T., and Panaro, M.A., Cytokine Growth Factor Rev., 2017, article ID S1359610117301119. https://doi.org/10.1016/j.cytogfr.2017.07.005

  2. Zhu, X., Bai, J., Liu, P., Wang, X., and Jiang, P., Sci. Rep., 2016, vol. 6, pp. 32538–32548. https://doi.org/10.1038/srep32538

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Zhao, X., Huo, R., Song, W., and Xu, T., Dev. Comp. Immunol., 2017, vol. 78, pp. 124–131. https://doi.org/10.1016/j.dci.2017.09.020

    Article  CAS  PubMed  Google Scholar 

  4. Linossi, E.M., Chandrashekaran, I.R., Kolesnik, T.B., Murphy, J.M., Webb, A.I., Willson, T.A., Kedzierski, L., Bullock, A.N., Babon, J.J., Norton, R.S., Nicola, N.A., and Nicholson, S.E., PLoS One, 2013, vol. 8, article ID e70536. https://doi.org/10.1371/journal.pone.0070536

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Zhao, X.Y., Zhao, S.S., Zheng, G.D., and Zhou, J.G., Gen. Comp. Endocrinol., 2019, vol. 284, pp. 113243–113251. https://doi.org/10.1016/j.ygcen.2019.113243

    Article  CAS  PubMed  Google Scholar 

  6. Huo, R., Chu, Q., Zhao, X., Liu, X., and Xu, T., Dev. Comp. Immunol., 2020, vol. 110, pp. 103723–103736. https://doi.org/10.1016/j.dci.2020.103723

    Article  CAS  PubMed  Google Scholar 

  7. Xia, T., Zhang, L., Sun, G., Yang, X.F., and Zhang, H.H., Peer J., 2021, vol. 9, article ID e11677. https://doi.org/10.7717/peerj.11677

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Yu, J., Sun, J., Zhao, S., Wang, H., and Zeng, Q., Fish Shellfish Immunol., 2019, vol. 93, pp. 223–231. https://doi.org/10.1016/j.fsi.2019.07.036

    Article  CAS  PubMed  Google Scholar 

  9. Ye, H.M., Zhao, T., Wu, L.X., Cheng, J., and Tan, X.Y., Fish Shellfish Immunol., 2019, vol. 86, pp. 906–912. https://doi.org/10.1016/j.fsi.2018.12.037

    Article  CAS  PubMed  Google Scholar 

  10. Hu, Q., Meng, Y., Wang, D., Tian, H.F., and Xiao, H.B., Genomics, 2019, vol. 111, pp. 1351–1359. https://doi.org/10.1016/j.ygeno.2018.09.007

    Article  CAS  PubMed  Google Scholar 

  11. Wang, Z., Hang, P., Zhang, Q., Xu, Q., and Qi, Z., Electr. J. Biotechnol., 2018, vol. 32, pp. 47–54. https://doi.org/10.1016/j.ejbt.2018.01.002

    Article  CAS  Google Scholar 

  12. Xu, Y.P., Zhou, Y.L., Xiao, Y., Gu, W.B., and Shu, M.A., Dev. Comp. Immunol., 2021, vol. 119, pp. 104015–104029. https://doi.org/10.1016/j.dci.2021.104015

    Article  CAS  PubMed  Google Scholar 

  13. Chen, C.Y., Chen, S.T., Juan, H.F., and Huang, H.C., Bioinformatics, 2012, vol. 28, pp. 3178–3181. https://doi.org/10.1093/bioinformatics/bts623

    Article  CAS  PubMed  Google Scholar 

  14. Peter, H., Birgit, H, Christian, T., Blum, H.E., and Wolf-Bernhard, O., Nucleic Acids Res., 2005, vol. 33, pp. 114–1275. https://doi.org/10.1093/nar/gki155

    Article  CAS  Google Scholar 

  15. Patnaik, B.B., Kim, B.B., Jo, Y.H., and Bang, I.S., Insects, 2019, vol. 10, pp. 76–95. https://doi.org/10.3390/insects10030076

    Article  PubMed Central  Google Scholar 

  16. Yao, J., Mu, W.J., Liu, S.K., Zhang, J.R., and We, H.S., Mol. Immunol., 2015, vol. 64, pp. 276–284. https://doi.org/10.1016/j.molimm.2014.12.003

    Article  CAS  PubMed  Google Scholar 

  17. Watanabe, H., Kubo, M., Numata, K., Takagi, K., and Matsukawa, A., J. Immunol., 2007, vol. 177, pp. 8650–8657. https://doi.org/10.1016/j.molimm.2014.12.003

    Article  CAS  Google Scholar 

  18. Kotob, M.H., Gokhlesh, K., Mona, S., Bartolomeo, G., Mahmoud, A., and Mansour, E.M., Parasites Vectors, 2018, vol. 11, pp. 326–338. https://doi.org/10.1186/s13071-018-2912-7

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  19. Zhang, J., Li, Z., Huang, J., Yin, H., Tian, J., and Qu, L., Veterinary Microbiol., 2020, vol. 245, pp. 108707–108723. https://doi.org/10.1016/j.vetmic.2020.108707

    Article  CAS  Google Scholar 

  20. Kedzierski, L., Tate, M.D., Hsu, A.C., Kolesnik, T.B., Linossi, E.M., Dagley, L., Dong, Z., Freeman, S., Infusini, G., Starkey, M.R., Bird, N.L., Chatfield, S.M., Babon, J.J., Huntington, N., Belz, G., Webb, A., Wark, P.A., Nicola, N.A., Xu, J., Kedzierska, K., Hansbro, P.M., and Nicholson, S.E., Elife, 2017, vol. 6, article ID e20444. https://doi.org/10.7554/eLife.20444

    Article  PubMed  PubMed Central  Google Scholar 

  21. Fu, M., Wang, C.B., Chen, C.X., He, Z.Y., Wang, Y.Q., Li, X.Q., Cao, H., Zheng, S.J., J. Virol., 2017, vol. 92, pp. 1646–1672. https://doi.org/10.1128/JVI.01646-17

    Article  Google Scholar 

  22. Gao, X.C., Niu, S.H., Huang, Y., Xiong, J.L., and Ren, H.T., Russ. J. Bioorg. Chem., 2021, vol. 47, pp. 252–260. https://doi.org/10.1134/S1068162021010064

    Article  Google Scholar 

  23. Gao, X.C., Zheng, X.D., Gao, S.Y., Huang, Y., Xiong, J.L., and Ren, H.T., Chemosphere, 2021, vol. 282, pp. 131133–131142. https://doi.org/10.1016/j.chemosphere.2021.131133

    Article  CAS  PubMed  Google Scholar 

  24. Mogilenko, D.A., Shavva, V.S., Dizhe, E.B., and Orlov, S.V., Mol. Biol., 2019, vol. 53, pp. 427–437. https://doi.org/10.1134/S0026893319030129

    Article  CAS  Google Scholar 

  25. Lapa, S.A., Klochikhina, E.S., Miftakhov, R.A., Zolotov, A.M., and Chudinov, A.V., Russ. J. Bioorg. Chem., 2020, vol. 46, pp. 859–861. https://doi.org/10.1134/S1068162020050131

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  26. Rodriguez-Nunez, I., Wcisel, D.J., Litman, R.T., Litman, G.W., and Yoder, J.A., Immunogenetics, 2016, vol. 68, pp. 295–312. https://doi.org/10.1007/s00251-016-0901-6

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  27. Hu, F., Chen, M., Zhang, Y., Wang, T., and Li, R., Russ. J. Bioorg. Chem., 2020, vol. 46, pp. 768–777. https://doi.org/10.1134/S1068162020050192

    Article  CAS  Google Scholar 

  28. Kantserova, N.P., Churova, M.V., Lysenko, L.A., Tushina, E.D., and Sukhovskaya, I.V., Russ. J. Bioorg. Chem., 2020, vol. 46, pp. 1181–1186. https://doi.org/10.1134/S1068162020060114

    Article  CAS  Google Scholar 

  29. Parshina, E. A., Zaraisky, A.G., and Martynova, N.Y., Russ. J. Bioorg. Chem., 2020, vol. 46, pp. 1172–1180. https://doi.org/10.1134/S1068162020060242

    Article  CAS  Google Scholar 

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Funding

This work was supported by the Doctoral Scientific Research Foundation of Henan University of Science and Technology, China (grant no. 13480079).

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Authors and Affiliations

  1. College of Animal Science and Technology, Henan University of Science and Technology, 471003, Luoyang, China

    X. C. Gao, Y. Huang, H. T. Ren & S. Y. Gao

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  1. X. C. Gao
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  2. Y. Huang
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Correspondence to X. C. Gao.

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COMPLIANCE WITH ETHICAL STANDARDS

The work was carried out on farmed A. davidianus in compliance with the international principles of the Declaration of Helsinki on the humane treatment of animals, the principles of humanity set forth in the European Community directive (86/609/EC), and the rules for working with experimental animals.

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The authors declare that they have no conflicts of interest.

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Gao, X.C., Huang, Y., Ren, H.T. et al. Identification of SOCS5 Gene in the Chinese Giant Salamander (Andrias davidianus) and Expression Profiles in Response to Citrobacter freundii Challenge. Russ J Bioorg Chem 48, 768–776 (2022). https://doi.org/10.1134/S1068162022040100

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