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Induction of Apoptosis in SKOV3 and DNA Binding by Cobalt(III) Polypyridyl Complexes

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Abstract

Three new Co(III) polypyridyl complexes [Co(phen)2CIIP]3+ {CIIP = 2-(5-chloro-3a,H-isoindol-3-yl)-1H-imidazo[4,5-f][1,10]phenantholine} (phen = 1,10 phenanthroline), [Co(bpy)2CIIP]3+ (bpy = 2,2′-bipyridine), and [Co(dmb)2CIIP]3+ (dmb = 4,4′-dimethyl-2,2′-bipyridine) were synthesized and characterized by different spectral methods. The complexes interact with DNA in an intercalation mode as confirmed by spectroscopic titration and viscosity measurements. All three complexes cleaved the pBR322 DNA in photoactivated cleavage studies and exhibited good antimicrobial activity. Anticancer activity of these Co(III) complexes was evaluated on the SKOV3 cell line. Cytotoxicity by MTT assay showed growth inhibition in dose dependent manner. Cell cycle analysis by flow cytometry data showed increase in Sub G1 population. Annexin V FITC/PI staining confirmed that these complexes caused cell death by the induction of apoptosis.

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ACKNOWLEDGMENTS

The authors are grateful to CFRD, Osmania University, for providing spectral studies. We are thankful to Dr. Ch. Mohan Rao and D. Kamakshi, CCMB, Hyderabad, for biological studies.

Funding

The authors are grateful to the UGC-UPE(FAR) Program Osmania University, Hyderabad, for funding.

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Authors and Affiliations

  1. Department of Chemistry, JNTU, 500007, Hyderabad, India

    Ch. Ravi

  2. Department of Chemistry, Osmania University, 500007, Hyderabad, India

    Ravi Kumar Vuradi, Srishailam Avudoddi & S. Satyanarayana

  3. Department of Biochemistry, Mahatma Gandhi University, 508254, Telangana, Nalgonda, India

    M. Ramchander

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  1. Ch. Ravi
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  2. Ravi Kumar Vuradi
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  5. S. Satyanarayana
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Correspondence to S. Satyanarayana.

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This article does not contain any studies involving human participants performed by any of the authors and does not contain any studies involving animals performed by any of the authors.

Conflicts of Interest. The authors declare that they have no conflicts of interest.

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Corresponding author: e-mail: ssnsirasani@gmail.com.

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Ravi, C., Vuradi, R.K., Avudoddi, S. et al. Induction of Apoptosis in SKOV3 and DNA Binding by Cobalt(III) Polypyridyl Complexes. Russ J Bioorg Chem 45, 273–284 (2019). https://doi.org/10.1134/S1068162019040095

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