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Light stress photodynamics of chlorophyll-binding proteins in Arabidopsis thaliana thylakoid membranes revealed by high-resolution mass spectrometric studies

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Abstract

In higher plants the light energy is captured by the photosynthetic pigments that are bound to photosystem I and II and their light-harvesting complex (LHC) subunits. In this study, we examined the photodynamic changes within chlorophyll-protein complexes in the thylakoid membrane of Arabidopsis thaliana leaves adapted to low light and subsequently exposed to light stress. Chlorophyll-protein complexes were isolated using sucrose density gradient centrifugation and blue-native polyacrylamid gel electrophoresis (BN-PAGE). Proteome analysis was performed using SDS-PAGE, HPLC, and high resolution mass spectrometry. We identified several rarely expressed and stress-induced chlorophyll-binding proteins, showed changes in localization of early light-induced protein family and LHC protein family members between different photosynthetic complexes and assembled/disassembled subcomplexes under light stress conditions and discuss their role in a variety of light stress-related processes.

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Abbreviations

BN:

blue-native

CID:

collision-induced dissociation

DM:

N-dodecyl-β-D-maltoside

FTICR-MS:

Fourier transform ion cyclotron resonance mass spectrometry

HL:

high light

LHC:

light-harvesting complex

LL:

low light

MALDI:

matrix assisted laser desorption/ionisation

PSI and PSII:

photosystem I and II, respectively

ROS:

reactive oxygen species

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Authors and Affiliations

  1. Institute of Biochemical Physics, Russian Academy of Sciences, Moscow, 119334, Russia

    D. N. Galetskiy, A. S. Kononikhin, I. A. Popov & E. N. Nikolaev

  2. Department of Physiology and Plant Biochemistry, University of Konstanz, DE-78457, Konstanz, Germany

    D. N. Galetskiy, J. N. Lohscheider & I. Adamska

  3. Institute for Energy Problems of Chemical Physics, Russian Academy of Sciences, Moscow, 119334, Russia

    A. S. Kononikhin, O. N. Kharybin, I. A. Popov & E. N. Nikolaev

Authors
  1. D. N. Galetskiy
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  2. J. N. Lohscheider
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  3. A. S. Kononikhin
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  4. O. N. Kharybin
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  5. I. A. Popov
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  6. I. Adamska
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  7. E. N. Nikolaev
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Correspondence to E. N. Nikolaev.

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Galetskiy, D.N., Lohscheider, J.N., Kononikhin, A.S. et al. Light stress photodynamics of chlorophyll-binding proteins in Arabidopsis thaliana thylakoid membranes revealed by high-resolution mass spectrometric studies. Russ J Bioorg Chem 37, 105–118 (2011). https://doi.org/10.1134/S1068162011010079

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  • DOI: https://doi.org/10.1134/S1068162011010079

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