Abstract
A fluorescent analogue of antibiotic actinomycin D, 7-aminoactinomycin D (7AAMD), which is widely used in molecular biology, was shown by steady-state, polarization, and phase fluorescent spectroscopy to bind primarily in the unwound regions of DNA with concomitant increase in its emission intensity. The maximum emission intensity of 7AAMD is observed for denatured DNA. Thus, 7AAMD may serve as a good indicator of DNA unwinding, denaturation, and fragmentation.
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Abbreviations
- 7AAMD:
-
7-aminoactinomycin D
- AMD:
-
actinomycin D
References
Hoon, M.A., Adler, E., Lindemeier, J., Battey, J.F., Ryba, N.J., and Zuker, C.S., Cell, 1999, vol. 96, pp. 541–551.
Ninomiya, Y., Proc. Natl. Acad. Sci. USA, 1998, vol. 95, pp. 5347–5350.
Chandrashekar, J., Hoon, M.A., Ryba, N.J., and Zuker, C.S., Nature, 2006, vol. 444, pp. 288–294.
Nelson, G., Hoon, M.A., Chandrashekar, J., Zhang, Y., Ryba, N.J., and Zuker, C.S., Cell, 2001, vol. 106, pp. 381–390.
Montmayeur, J.P., Liberles, S.D., Matsunami, H., and Buck, L.B., Nat. Neurosci, 2001, vol. 4, pp. 492–498.
Max, M., Shanker, Y.G., Huang, L., Rong, M., Liu, Z., Campagne, F., Weinstein, H., Damak, S., and Margolskee, R.F., Nat. Genet., 2001, vol. 28, pp. 58–63.
Kitagawa, M., Kusakabe, Y., Miura, H., Ninomiya, Y., and Hino, A., Biochem. Biophys. Res. Commun., 2001, vol. 283, pp. 236–242.
Sainz, E., Korley, J.N., Battey, J.F., and Sullivan, S.L., J. Neurochem., 2001, vol. 77, pp. 896–903.
Reed, D.R., Li, S., Li, X., Huang, L., Tordoff, M.G., Starling-Roney, R., Taniguchi, K., West, D.B., Ohmen, J.D., Beauchamp, G.K., and Bachmanov, A.A., J. Neurosci., 2004, vol. 24, pp. 938–946.
Li, X., Staszewski, L., Xu, H., Durick, K., Zoller, M., and Adler, E., Proc. Natl. Acad. Sci. USA, 2002, vol. 99, pp. 4692–4696.
Zhao, G.Q., Zhang, Y., Hoon, M.A., Chandrashekar, J., Erlenbach, I., Ryba, N.J., and Zuker, C.S., Cell, 2003, vol. 115, pp. 255–266.
Li, X., Li, W., Wang, H., Cao, J., Maehashi, K., Huang, L., Bachmanov, A.A., Reed, D.R., Legrand-Defretin, V., Beauchamp, G.K., and Brand, J.G., PLoS Genet., 2005, vol. 1, pp. 27–35.
Xu, H., Staszewski, L., Tang, H., Adler, E., Zoller, M., and Li, X., Proc. Natl. Acad. Sci. USA, 2004, vol. 101, pp. 14 258–14 263.
Ikeda, K., J. Tokyo Chem. Soc, 1909, vol. 30, pp. 820–836.
Yamaguchi, S., J. Food Sci, 1967, vol. 32, pp. 473–478.
Nelson, G., Chandrashekar, J., Hoon, M.A., Feng, L., Zhao, G., Ryba, N.J., and Zuker, C.S., Nature, 2002, vol. 416, pp. 199–202.
Adler, E., Hoon, M.A., Mueller, K.L., Chandrashekar, J., Ryba, N.J., and Zuker, C.S., Cell, 2000, vol. 100, pp. 693–702.
Chandrashekar, J., Mueller, K.L., Hoon, M.A., Adler, E., Feng, L., Guo, W., Zuker, C.S., and Ryba, N.J., Cell, 2000, vol. 100, pp. 703–711.
Bufe, B., Hofmann, T., Krautwurst, D., Raguse, J.D., and Meyerhof, W., Nat. Genet., 2002, vol. 32, pp. 397–401.
Kim, U.K., Jorgenson, E., Coon, H., Leppert, M., Risch, N., and Drayna, D., Science, 2003, vol. 299, pp. 1221–1225.
Pronin, A.N., Tang, H., Connor, J., and Keung, W., Chem. Senses, 2004, vol. 29, pp. 583–593.
Sainz, E., Cavenagh, M.M., Gutierrez, J., Battey, J.F., Northup, J.K., and Sullivan, S.L., Biochem. J., 2007, vol. 403, pp. 537–543.
Mueller, K.L., Hoon, M.A., Erlenbach, I., Chandrashekar, J., Zuker, C.S., and Ryba, N.J., Nature, 2005, vol. 434, pp. 225–229.
Finger, T.E., Bottger, B., Hansen, A., Anderson, K.T., Alimohammadi, H., and Silver, W.L., Proc. Natl. Acad. Sci. USA, 2003, vol. 100, pp. 8981–8986.
Wu, S.V., Rozengurt, N., Yang, M., Young, S.H., Sinnett-Smith, J., and Rozengurt, E., Proc. Natl. Acad. Sci. USA, 2002, vol. 99, pp. 2392–2397.
Zhang, Y., Hoon, M.A., Chandrashekar, J., Mueller, K.L., Cook, B., Wu, D., Zuker, C.S., and Ryba, N.J., Cell, 2003, vol. 112, pp. 293–301.
McLaughlin, S.K., McKinnon, P.J., and Margolskee, R.F., Nature, 1992, vol. 357, pp. 563–569.
Wong, G.T., Gannon, K.S., and Margolskee, R.F., Nature, 1996, vol. 381, pp. 796–800.
He, W., Yasumatsu, K., Varadarajan, V., Yamada, A., Lem, J., Ninomiya, Y., Margolskee, R.F., and Damak, S., J. Neurosci., 2004, vol. 24, pp. 7674–7680.
Liman, E.R., Handb. Exp. Pharmacol., 2007, vol. 179, pp. 287–298.
Oike, H., Wakamori, M., Mori, Y., Nakanishi, H., Taguchi, R., Misaka, T., Matsumoto, I., and Abe, K., Biochim. Biophys. Acta, 2006, vol. 1761, pp. 1078–1084.
Talavera, K., Yasumatsu, K., Voets, T., Droogmans, G., Shigemura, N., Ninomiya, Y., Margolskee, R.F., and Nilius, B., Nature, 2005, vol. 438, pp. 1022–1025.
Damak, S., Rong, M., Yasumatsu, K., Kokrashvili, Z., Perez, C.A., Shigemura, N., Yoshida, R., Mosinger, B., Glendinning, J.I., Ninomiya, Y., and Margolskee, R.F., Chem. Senses, 2006, vol. 31, pp. 253–264.
Avenet, P. and Lindemann, B., J. Membr. Biol., 1988, vol. 105, pp. 245–255.
Doolin, R.E. and Gilbertson, T.A., J. Gen. Physiol., 1996, vol. 107, pp. 545–554.
Heck, G.L., Mierson, S., and DeSimone, J.A., Science, 1984, vol. 223, pp. 403–405.
Ninomiya, Y., J. Neurophysiol., 1996, vol. 76, pp. 3550–3554.
Lundy, R.F., Pittman, D.W., and Contreras, R.J., Am. J. Physiol., 1997, vol. 273, pp. R1923–R1931.
Kretz, O., Barbry, P., Bock, R., and Lindemann, B., J. Histochem. Cytochem., 1999, vol. 47, pp. 51–64.
Lyall, V., Heck, G.L., Vinnikova, A.K., Ghosh, S., Phan, T.H., Alam, R.I., Russell, O.F., Malik, S.A., Bigbee, J.W., and DeSimone, J.A., J. Physiol., 2004, vol. 558, pp. 147–159.
Lyall, V., Heck, G.L., Vinnikova, A.K., Ghosh, S., Phan, T.H., and Desimone, J.A., Chem. Senses, 2005, vol. 30, Suppl. 1, pp. i42–i43.
Ruiz, C., Gutknecht, S., Delay, E., and Kinnamon, S., Chem. Senses, 2006, vol. 31, pp. 813–820.
Li, X.J., Blackshaw, S., and Snyder, S.H., Proc. Natl. Acad. Sci. USA, 1994, vol. 91, pp. 1814–1818.
Okada, Y., Miyamoto, T., and Sato, T., Comp. Biochem. Physiol. A, 1990, vol. 97, pp. 535–536.
Herness, M.S., Comp. Biochem. Physiol. Comp. Physiol, 1992, vol. 103, pp. 269–273.
Lin, W., Finger, T.E., Rossier, B.C., and Kinnamon, S.C., J. Comp. Neurol., 1999, vol. 405, pp. 406–420.
Okada, Y., Miyamoto, T., and Sato, T., Comp. Biochem. Physiol. A, 1991, vol. 100, pp. 693–696.
DeSimone, J.A., Lyall, V., Heck, G.L., and Feldman, G.M., Respir. Physiol., 2001, vol. 129, pp. 231–245.
Shimada, S., Ueda, T., Ishida, Y., Yamamoto, T., and Ugawa, S., Arch. Histol. Cytol., 2006, vol. 69, pp. 227–231.
Jasti, J., Furukawa, H., Gonzales, E.B., and Gouaux, E., Nature, 2007, vol. 449, pp. 316–323.
Richter, T.A., Dvoryanchikov, G.A., Roper, S.D., and Chaudhari, N., J. Neurosci., 2004, vol. 24, pp. 4088–4091.
Clapham, D.E., Nature, 2003, vol. 426, pp. 517–524.
Ishimaru, Y., Inada, H., Kubota, M., Zhuang, H., Tominaga, M., and Matsunami, H., Proc. Natl. Acad. Sci. USA, 2006, vol. 103, pp. 12 569–12 574.
Huang, A.L., Chen, X., Hoon, M.A., Chandrashekar, J., Guo, W., Trankner, D., Ryba, N.J., and Zuker, C.S., Nature, 2006, vol. 442, pp. 934–938.
Laugerette, F., Passilly-Degrace, P., Patris, B., Niot, I., Febbraio, M., Montmayeur, J.P., and Besnard, P., J. Clin. Invest., 2005, vol. 115, pp. 3177–3184.
McCormack, D.N., Clyburn, V.L., and Pittman, D.W., Physiol. Behav, 2006, vol. 87, pp. 582–594.
Fukuwatari, T., Kawada, T., Tsuruta, M., Hiraoka, T., Iwanaga, T., Sugimoto, E., and Fushiki, T., FEBS Lett., 1997, vol. 414, pp. 461–464.
Matsumura, S., Mizushige, T., Yoneda, T., Iwanaga, T., Tsuzuki, S., Inoue, K., and Fushiki, T., Biomed. Res., 2007, vol. 28, pp. 49–55.
Gotoh, C., Hong, Y.H., Iga, T., Hishikawa, D., Suzuki, Y., Song, S.H., Choi, K.C., Adachi, T., Hirasawa, A., Tsujimoto, G., Sasaki, S., and Roh, S.G., Biochem. Biophys. Res. Commun., 2007, vol. 354, pp. 591–597.
Pittman, D.W., Labban, C.E., Anderson, A.A., and O’Connor, H.E., Chem. Senses, 2006, vol. 31, pp. 835–843.
Chale-Rush, A., Burgess, J.R., and Mattes, R.D., Chem. Senses, 2007, vol. 32, pp. 423–431.
Caicedo, A., Kim, K.N., and Roper, S.D., J. Physiol., 2002, vol. 544, pp. 501–509.
Scott, K., Brady, R., Cravchik, A., Morozov, P., Rzhetsky, A., Zuker, C., and Axel, R., Cell, 2001, vol. 104, pp. 661–673.
Clyne, P.J., Warr, C.G., and Carlson, J.R., Science, 2000, vol. 287, pp. 1830–1834.
Dunipace, L., Meister, S., McNealy, C., and Amrein, H., Curr. Biol., 2001, vol. 11, pp. 822–835.
Wang, Z., Singhvi, A., Kong, P., and Scott, K., Cell, 2004, vol. 117, pp. 981–991.
Ueno, K., Ohta, M., Morita, H., Mikuni, Y., Nakajima, S., Yamamoto, K., and Isono, K., Curr. Biol., 2001, vol. 11, pp. 1451–1455.
Chyb, S., Dahanukar, A., Wickens, A., and Carlson, J.R., Proc. Natl. Acad. Sci. USA, 2003, vol. 100, Suppl 2, pp. 14 526–14 530.
Moon, S.J., Kottgen, M., Jiao, Y., Xu, H., and Montell, C., Curr. Biol., 2006, vol. 16, pp. 1812–1817.
Thorne, N., Chromey, C., Bray, S., and Amrein, H., Curr. Biol., 2004, vol. 14, pp. 1065–1079.
Marella, S., Fischler, W., Kong, P., Asgarian, S., Rueckert, E., and Scott, K., Neuron, 2006, vol. 49, pp. 285–295.
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Original Russian Text © M.A. Bitekhtina, N.L. Vekshin, 2008, published in Bioorganicheskaya Khimiya, 2008, Vol. 34, No. 6, pp. 781–785.
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Bitekhtina, M.A., Vekshin, N.L. 7-Aminoactinomycin as a fluorescent probe for DNA unwinding and denaturation. Russ J Bioorg Chem 34, 698–703 (2008). https://doi.org/10.1134/S1068162008060083
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DOI: https://doi.org/10.1134/S1068162008060083