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Synthesis of 99mTc-L-carnitine as a model for tumor imaging

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Abstract

An L-carnitine derivative labeled with 99mTc was prepared. It is effective in tumor imaging. The labeling was done using SnCl2 as a reducing agent. The optimum conditions required to label 25 μg of L-carnitine were as follows: 100 μg of SnCl2, 30 min reaction time, room temperature, pH 7 (0.5 M phosphate buffer). The radiochemical purity of the labeled compound was determined by paper chromatography. The yield was about 93%. About 2.5 × 106 Ehrlich ascites carcinoma (EAC) cells were injected intraperitoneally to produce ascites and intramuscularly in the right thigh to produce solid tumor in female mice. Biodistribution studies were carried out by injecting a solution of 99mTc-L-carnitine into normal and tumor-bearing mice. The uptake in ascites and in solid tumor was over 5% of the injected dose per gram tissue at 4 h post injection. These data revealed localization of the tracer in the tumor tissues with high percentage sufficient to use 99mTc-L-carnitine as a promising tool for diagnosis of tumor.

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Authors and Affiliations

  1. Labelled Compounds Department, Radioisotopes Production and Radioactive Sources Division, Hot Laboratories Center, Atomic Energy Authority, P.O. Box 13759, Cairo, Egypt

    I. T. Ibrahim

  2. Department of Pharmaceutics, Faculty of Pharmacy, Umm El-Qurah University, Makkah, Saudi Arabia

    K. M. Attallah

Authors
  1. I. T. Ibrahim
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  2. K. M. Attallah
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Correspondence to I. T. Ibrahim.

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Published in Russian in Radiokhimiya, 2012, Vol. 54, No. 4, pp. 374–378.

The text was submitted by the authors in English.

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Ibrahim, I.T., Attallah, K.M. Synthesis of 99mTc-L-carnitine as a model for tumor imaging. Radiochemistry 54, 407–411 (2012). https://doi.org/10.1134/S1066362212040170

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  • DOI: https://doi.org/10.1134/S1066362212040170

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