Abstract
Dielectric and optical methods were used to reveal the complex nature of globule–coil–coil associate phase transitions in solutions of human serum albumin. The impedancemetric and nephelometric curves of temperature dependences of the parameters of the phase transitions in albumin that was introduced into the standard saline were obtained. Impedancemetry revealed a two-stage mechanism of the globule–coil phase transition, and nephelometry established a two-stage mechanism of the coil–coil associate phase transition. The concentration dependences of the critical indices and temperatures were determined for both phase transitions.
Similar content being viewed by others
References
S. N. Illarioshkin, Conformational Brain Disorders (Yanus-K, Moscow, 2003).
N. E. Ivanova, E. B. Shadrin, N. L. Vas’kova, and M. E. Pashkevich, Usp. Sovrem. Estestvozn., No. 1–4, 568 (2015).
M. V. Vol’kenshtein, Biophysics (Nauka, Moscow, 1988)
L. I. Krishtalik, Phys.-Usp. 55, 1192 (2012).
A. V. Il’inskii, N. E. Ivanova, E. B. Shadrin, and N. L. Yutkina, RF Patent No. 2205392 (2003).
S. G. Kalashnikov, Electricity (Fizmatlit, Moscow, 2003).
A. L. Efros, Physics and Geometry of Disorder (Nauka, Moscow, 1982).
A. N. Vasil’ev, Quantum-Field Renormalization Group in the Critical Behavior Theory and Stochastic Dynamics (Peterb. Inst. Yad. Fiz., St. Petersburg, 1998).
N. Gavish and K. Promislow, Phys. Rev. E 94, 012611 (2016).
M. A. Ziganshin and V. V. Gorbachuk, Lectures on Physical and Colloid Chemistry (Kazan. Univ., Kazan, 2007)
I. A. Semiokhin, Physical Chemistry: Textbook (Mosk. Gos. Univ., Moscow, 2001).
G. Hoffner and P. Djian, Biochimie 84, 273 (2002).
A. V. Finkel’shtein and O. B. Ptitsyn, Protein Chemistry (KDU, Moscow, 2012).
A. Yu. Grosberg and A. R. Khokhlov, Statistical Physics of Macromolecules (Nauka, Moscow, 1989).
L. D. Landau and E. M. Lifshitz, Statistical Physics (Nauka, Moscow, 1988).
V. F. Gantmakher and V. T. Dolgopolov, Phys.-Usp. 51, 3 (2008).
A. V. Finkel’shtein, Physics of Protein Molecules (Inst. Komp’yut. Issled., Moscow, 2014).
B. I. Shklovskii and A. L. Efros, Sov. Phys. Usp. 18, 845 (1975).
A. L. Efros, Physics and Geometry of Disorder (Nauka, Moscow, 1982).
A. S. Davydov, Biology and Quantum Mechanics (Naukova Dumka, Kiev, 1979).
N. E. Ivanova, E. B. Shadrin, and N. L. Vas’kova, Sarat. Nauchno-Med. Zh. 9 (1), 71 (2013).
V. M. Kapralova, E. A. Nazarova, N. E. Ivanova, and E. B. Shadrin, Nauchno-Tekh. Vedomosti S.-Peterb. Gos. Politekh. Univ. Fiz.-Mat. Nauki, No. 3, 83 (2012).
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © A.O. Vonti, A.V. Il’inskii, V.M. Kapralova, E.B. Shadrin, 2018, published in Zhurnal Tekhnicheskoi Fiziki, 2018, Vol. 88, No. 6, pp. 934–942.
Rights and permissions
About this article
Cite this article
Vonti, A.O., Il’inskii, A.V., Kapralova, V.M. et al. The Complex Nature of Thermal Phase Transitions in Albumin Solutions. Tech. Phys. 63, 908–915 (2018). https://doi.org/10.1134/S1063784218060245
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1063784218060245