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Characterization of oligomerization of a peptide from the ebola virus glycoprotein by small-angle neutron scattering

Abstract

Transmission electron microscopy (TEM) and small-angle neutron scattering (SANS) studies showed that model peptides QNALVCGLRQ (G33) and QNALVCGLRG (G31) corresponding to region 551–560 of the GP protein of the Sudan Ebola virus are prone to oligomerization in solution. Both peptides can form amyloid-like fibrills. The G33 peptide forms fibrils within one day of incubation, whereas the fibrillogenesis of the G31 peptide is observed only after incubation for several months. The possible role of the observed processes in the pathogenesis and the possibility of applying a combination of the TEM and SANS techniques to search for new compounds that are able to influence the protein oligomerization are discussed.

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References

  1. J. D. Cook and J. E. Lee, PLoS Pathog. 9 (5), e1003258 (2013).

    Article  Google Scholar 

  2. Y. M. Ostanevich, Makromol. Chem., Macromol. Symp. 15 (1), 91 (1988).

    Article  Google Scholar 

  3. V. V. Egorov, M. I. Dyukov, A. A. Shaldzhyan, et al., Innovatsii, No. 6, 84 (2008).

    Google Scholar 

  4. V. Egorov, O. Matusevich, A. Shaldzhyan, et al., Int. J. Pept. 2013, 1 (2013).

    Article  Google Scholar 

  5. O. V. Matusevich, V. V. Egorov, I. A. Gluzdikov, et al., Antiviral Res. 2014. http://dx.doi.org/. doi 10.1016/j.antiviral.2014.10.015

    Google Scholar 

  6. V. V. Egorov, K. V. Solovyov, N. A. Grudinina, et al., Protein Pept. Lett. 14 (5), 471 (2007).

    Article  Google Scholar 

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Correspondence to V. V. Egorov.

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Original Russian Text © V.V. Egorov, A.N. Gorshkov, T.N. Murugova, A.V. Vasin, D.V. Lebedev, V.V. Isaev-Ivanov, O.I. Kiselev, 2016, published in Kristallografiya, 2016, Vol. 61, No. 1, pp. 103–106.

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Egorov, V.V., Gorshkov, A.N., Murugova, T.N. et al. Characterization of oligomerization of a peptide from the ebola virus glycoprotein by small-angle neutron scattering. Crystallogr. Rep. 61, 94–97 (2016). https://doi.org/10.1134/S1063774516010065

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  • DOI: https://doi.org/10.1134/S1063774516010065

Keywords

  • Fibril
  • Crystallography Report
  • Transmission Electron Microscopy Experiment
  • Transmission Electron Microscopy Copper Grid
  • Thin Fibril