Abstract
The phospholipid and fatty acid composition and thermotropic behavior of total lipids were studied in the metal-accumulating marine strain Pseudomonas putida IB28 grown in the presence of Cu2+ and Cd2+ at 4 and 24°C. Despite the changes in acidic lipid content, unsaturated/saturated fatty acid ratio, and cyclopropane fatty acid level, the temperature range of calorimetric phase transitions of bacterial total lipids was slightly altered under these factors. The suppressive action of heavy metals on bacterial growth is attributable to the phase separation of lipids and, as a consequence, to a sharp increase in the ion permeability of the lipid bilayer. The increase in acidic phospholipid level under the influence of Cu2+ and Cd2+, especially at 24°C, is likely to be indicative of their complexation with heavy metal ions.
Access this article
We’re sorry, something doesn't seem to be working properly.
Please try refreshing the page. If that doesn't work, please contact support so we can address the problem.
Similar content being viewed by others
References
Bezverbnaya, I.P., Dimitrieva, G.Yu., Tazaki, K., and Watanabe, H., An Experience of Evaluation of Near-Shore Seawaters in Primorye Based on Microbial Indication, Vodnye Resursy, 2003, no. 2, pp. 222–231.
Ivkov, V.G. and Berestovsky, G.N., Dinamicheskaya struktura lipidnogo bisloya (Dynamic Structure of Lipid Bilayer), Moscow: Nauka, 1981.
Nikolaev, Yu.A., Prosser, J.I., and Panikov, N.S., Extracellular Factors of Adaptation to Unfavorable Conditions in a Periodic Culture of Pseudomonas fluorescens, Mikrobiologiya, 2000, vol. 69, no. 5, pp. 629–635.
Abbas, C.A. and Card, G.L., The Relationships between Growth Temperature, Fatty Acid Composition and the Physical State and Fluidity of Membrane Lipids in Yersinia enterocolitica, Biochim. Biophys. Acta, 1980, vol. 602, no. 3. pp. 469–476.
Bakholdina, S.I., Sanina, N.M., Krasikova, I.N., et al., The Impact of Abiotic Factors (Temperature and Glucose) on Physicochemical Properties of Lipids from Yersinia pseudotuberculosis, Biochimie, 2004, vol. 86, pp. 875–881.
Barton, P.G., The Influence of Surface Charge Density of Phosphatides on the Binding of Some Cations, J. Biol. Chem., 1968, vol. 243, pp. 3884–3890.
Beney, L. and Gervais, P., Influence of the Fluidity of the Membrane on the Response of Microorganisms to Environmental Stresses, Appl. Microbiol. Biotechnol., 2001, vol. 57, no. 1–2, pp. 34–42.
Beney, L., Mille, Y., and Gervais, P., Death of Escherichia coli during Rapid and Severe Dehydration is Related to Lipid Phase Transition, Appl. Microbiol. Biotechnol., 2004, vol. 65, pp. 457–464.
Cervantes, C. and Gutierez-Corona, F., Copper Resistance Mechanisms in Bacteria and Fungi, FEMS Microbiol. Rev., 1994, vol. 14, no. 2, pp. 23–30.
Domènech, O., Morros, A., Cabañas, M.E., et al., Supported Planar Bilayers from Hexagonal Phases, Biochim. Biophys. Acta, 2007, vol. 1768, pp. 100–106.
Härtig, C., Loffhagen, N., and Harms, H., Formation of Trans Fatty Acids Is Not Involved in Growth-Linked Membrane Adaptation of Pseudomonas putida, Appl. Environ. Microbiol., 2005, vol. 71, pp. 1915–1922.
Hazel, J.R. and Williams, E.E., The Role of Alterations in Membrane Lipid Composition in Enabling Physiological Adaptation of Organisms to Their Physical Environment, Prog. Lipid Res., 1990, vol. 29, pp. 167–227.
Jøstensen, J.-P. and Landfald, B., Influence of Growth Conditions on Fatty Acid Composition of a Polyunsaturated Fatty-Acid-producing Vibrio Species, Arch. Microbiol., 1996, vol. 165, pp. 306–310.
Julshamn, K. and Andersen, K.J., A Study on the Digestion of Human Muscle Biopsies for Trace Metal Analysis Using an Organic Tissue Solubilizer, Anal. Biochem., 1979, vol. 98, pp. 315–318.
Kozloff, L.M., Turner, M.A., Arellano, F., and Lute, M., Phosphatidylinositol, a Phospholipid of Ice-nucleating Bacteria, J. Bacteriol., 1991, vol. 173, pp. 2053–2060.
Kramer, J.K.G., Fouchard, R.C., and Jenkins, K.J., Differences in Chromatographic Properties of Fused Silica Capillary Columns, Coated, Crosslinked, Bonded, or Crosslinked and Bonded with Polyethylene Glycols (Carbowax 20M) Using Complex Fatty Acid Methyl Ester Mixtures, J. Chromatogr. Sci., 1985, vol. 23, no. 2. pp. 54–56.
Laddaga, R.A. and Silver, S., Cadmium Uptake in Escherichia coli K-12, J. Bacteriol., 1985, vol. 162, pp. 1100–1105.
Lewis, R. and McElhaney, R.N., Thermotropic Phase Behavior of Models Membranes Composed of Phosphatidylcholines Containing iso-Branched Fatty Acids. 1. Differential Scanning Calorimetric Study, Biophys. J., 2000, vol. 79, pp. 1455–1464.
McGarrity, J.T. and Armstrong, J.B., Phase Transition Behaviour of Artificial Liposomes Composed of Phosphatidylcholines Acylated with Cyclopropane Fatty Acids, Biochim. Biophys. Acta., 1981, vol. 640, pp. 544–548.
Medeot, D.B., Bueno, M.A., Dardanelli, M.S., and García de Lema, M., Adaptational Changes in Lipids of Bradyrhizobium SEMIA 6144 Nodulating Peanut as a Response to Growth Temperature and Salinity, Curr. Microbiol., 2007, vol. 54, pp. 31–35.
Meyer, J.-M., Pyoverdines: Pigments, Siderophores and Potential Taxonomic Markers of Fluorescent Pseudomonas Species, Arch. Microbiol., 2000, vol. 174, no. 3, pp. 135–152.
Morein, S., Andersson, A., Rilfors, L., and Lindblom, G., Wild-Type Escherichia coli Cells Regulate the Membrane Lipid Composition in a “Window” between Gel and non-Lamellar Structures, J. Biol. Chem., 1996, vol. 22, pp. 6801–6809.
Mouritsen, O.G. and Kinnunen, P.K.J., Role of Lipid Organisation and Dynamics for Membrane Fluidity, Biological Membranes, Boston: Birkhäuser, 1996, pp. 463–502.
Mueller, P., Rudin, D.O., Tien, H., and Wescott, W., Reconstitution of Cell Membrane Structure in vitro and Its Transformation into an Excitable System Nature, 1962, vol. 194, pp. 979–981.
Nagamachi, E., Shibuya, S., Hirai, Y., et al. Adaptational Changes of Fatty Acid Composition and Physical State of Membrane Lipids Following the Change of Growth Temperature in Yersinia enterocolitica, Microbiol. Immunol., 1991, vol. 35, no. 12, pp. 1085–1093.
Nies, D.H., Microbial Heavy-Metal Resistance, Appl. Microbiol. Biotechnol., 1999, vol. 51, pp. 730–750.
Park, J.B., Kim, H.J., Ryu, P.D., and Moczydlowski, E. Effect of Phosphatidylserine on Unitary Conductance and Ba2+ Block of the BK Ca2+-Activated K+ Channel: Reexamination of the Surface Charge Hypothesis, J. Gen. Physiol., 2003, vol. 121, P. 375–397.
Pinkart, H.C. and White, D.C., Phospholipid Biosynthesis and Solvent Tolerance in Pseudomonas putida Strains, J. Bacteriol., 1997, vol. 179, pp. 4219–4226.
Sanina, N.M. and Kostetsky, E.Y., Seasonal Changes in Thermotropic Behavior of Phosphatidylcholine and Phosphatidylethanolamine in Different Organs of the Ascidian Halocynthia aurantium, Comp. Biochem. Physiol., Ser. B., 2001, vol. 128, pp. 295–305.
Shiba, Y., Yokoyama, Y., Aono, Y., et al., Activation of the Rcs Signal Transduction System Is Responsible for the Thermosensitive Growth Defect of an Escherichia coli Mutant Lacking Phosphatidylglycerol and Cardiolipin, J. Bacteriol., 2004, vol. 186, no. 19, pp. 6526–6535.
Tang, Y. and Hollingsworth, R., Regulation of Lipid Synthesis in Bradyrhizobium japonicum: Low Oxygen Concentrations Trigger Phosphatidylinositol Biosynthesis, Appl. Environ. Microbiol., 1998, vol. 64, pp. 1963–1966.
Theuvenet, A.P.R., Kesseles, B.G.F., Blankensteijn, W.M., and Borst-Pawels, G.W.H.F., A Comparative Study of K+-Loss from a Cadmium-sensitive and a Cadmium-resistant Strain of Saccharomyces cerevisiae, FEMS Microbiol. Lett., 1987, vol. 43, pp. 147–153.
Vanounou, S., Parola, A.H., and Fishov, I., Phosphatidylethanolamine and Phosphatidylglycerol Are Segregated into Different Domains in Bacterial Membrane. A Study with Pyrene-Labelled Phospholipids, Mol. Microbiol., 2003, vol. 49, pp. 1067–1079.
Vaskovsky, V. and Khotimchenko, S., HPTLC of Polar Lipids of Algae and Other Plants, J. High Resol. Chromatogr., 1982, vol. 5, pp. 635–636.
Vaskovsky, V.E., Kostetsky, E.Y., and Vasendin, I.M., A Universal Reagent for Phospholipid Analysis, J. Chromatogr., 1975, vol. 114, pp. 129–141.
Vaskovsky, V.E. and Terekhova, T.A., HPTLC of Phospholipid Mixtures Containing Phosphatidylglycerol, J. High. Resol. Chromatogr., 1979, vol. 2, pp. 671–672.
Wang, L., Li, F., and Zhou, Q., Contribution of Cell-Surface Components to Cu2+ Adsorption by Pseudomonas putida 5-x, Appl. Biochem. Biotechnol., 2006, vol. 128, no. 1, pp. 33–46.
Williams, W.P., Cold Induced Lipid Phase Transitions, Phil. Trans. Roy. Soc. London, Ser. B: Biol. Sci., 1990, vol. 326, pp. 555–570.
Wolf, C., Koumanov, K., Tenchov, B., and Quinn, P.J., Cholesterol Favors Phase Separation of Sphingomyelin, Biophys. Chem., 2001, vol. 89, pp. 163–172.
Youchimizu, M. and Kimura, T., Study of Intestinal Microflora of Salmonids, Fish Pathol., 1976, vol. 10, pp. 243–259.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © O.B. Popova, N.M. Sanina, G.N. Likhatskaya, I.P. Bezverbnaya, 2008, published in Biologiya Morya.
Rights and permissions
About this article
Cite this article
Popova, O.B., Sanina, N.M., Likhatskaya, G.N. et al. Effects of copper and cadmium ions on the physicochemical properties of lipids of the marine bacterium Pseudomonas putida IB28 at different growth temperatures. Russ J Mar Biol 34, 179–185 (2008). https://doi.org/10.1134/S1063074008030073
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1063074008030073