Abstract
Cytomixis was recorded during microsporogenesis in sesame (Sesamum indicum L.), a member of the family Pedaliaceae. The phenomenon of cytomixis was observed at various stages of meiosis in 0.5% sodium azide (SA) treated populations of Sesamum indicum L. Cytomixis was observed to occur through various methods, i.e. by forming cytoplasmic channels and direct fusion of pollen mother cells (PMCs), the former was more frequent than the latter. The migration of nuclear content involved all the chromatin/chromosomes or part of it from donor to recipient cell/cells. Some completely empty meiocytes were also observed. Stickiness, precocious movement, laggards, unorientation and micronuclei were observed in almost all the sets treated with various doses of SA. Increase in the doses of SA had a positive effect on the percentage of PMCs showing cytomixis and chromosomal abnormalities. The impact of cytomixis on meiotic behaviour, reduced pollen viability and heterogeneous sized pollen grains were observed.
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Basavaiah, M.T.C.S., Cytomixis in Pollen Mother Cells of Urochloa panicoides P. Beauv. (Poaceae), Cytologia, 1987, vol. 52, pp. 69–74.
Bauchan, G.R., Cytomixis in Agropyron cristatum, Genome, 1987, vol. 29, pp. 765–769.
Bedi, Y.S., Cytomixis in Woody Species, Proc. Indian Natl. Sci. Acad. (Plant Sci.), 1990, vol. 100, no. B, pp. 233–238.
Bione, N.C.P., Pagliarini, M.S., and De Toledo, J.F.F., Meiotic Behavior of Several Brazilian Soybean Varieties, Genet. Mol. Biol., 2000, vol. 23, pp. 623–631.
Bobak, M. and Herich, R., Cytomixis as a Manifestation of Pathological Changes after the Application of Trifluraline, Nucleus, 1978, vol. 21, pp. 22–26.
Caetano-Pereira, C.M. and Pagliarini, M.S., Cytomixis in Maize Microsporocytes, Cytologia, 1997, vol. 62, pp. 351–355.
Cooper, D.D., The Transfer of Deoxyribose Nucleic Acid from the Tapetum to the Microsporocytes at the Onset of Meiosis, Cytologia, 1952, vol. 86, pp. 271–274.
F.A.O., FAOSTAT Data, Rome: Food and Agriculture Organization, 2004.
Falistocco, E., Tosti, N., and Falcinelli, M., Cytomixis in Pollen Mother Cells of Diploid Dactylis. One of the Origins of 2n Gametes, J. Hered., 1995, vol. 86, pp. 448–453.
Feijo, J. and Pais, S., Cytomixis in Meiosis during the Microsporogenesis of Ophris lutea: An Ultrastructural Study, Caryologia, 1995, vol. 42, no. 1, pp. 37–48.
Ghanma, A.M. and Talaat, A.A., Cytomixis and Its Possible Evolutionary Role in a Kuwaiti Population of Diplotaxis harra (Brassicaceae), Bot. J. Linnean Soc., 2003, vol. 143, pp. 169–175.
Heslop-Harrison, J., Cytoplasmic Connexions between Angiosperm Meiocytes, Ann. Bot., 1966, vol. 30, pp. 221–234.
I.A.E.A., Sesame Improvement by Induced Mutations, TECDOC 1195, 2001.
Kamal-Eldin, A., Sesamin (a Compound from Sesame Oil) Increases Tocopherol Levels in Rats Fed ad libitum, Lipids, 1995, vol. 30, pp. 499–505.
Kamra, O.P., Chromatin Extrusion and Cytomixis in Pollen Mother Cells of Hordeum, Hereditas, 1960, vol. 46, pp. 592–600.
Kornicke, M., Uber Ortsverannderung von Zellkernen, Niderrhein, S.B., Bonn: Ges Natur Heilkunde, 1901, vol. 8, pp. 14–25.
Kostritsyna, T.V. and Soldatov, I.V., Cytomixis in the Shoot Apical Meristem of Hybrids of Prunus domestica L. Persica vulgaris Mill, Genetika, 1991, vol. 27, no. 10, pp. 1790–1794.
Koul, K.K., Cytomixis in Pollen Mother Cells of Allopecurus arundinaceus Poir, Cytologia, 1990, vol. 55, pp. 169–173.
Mantu, D.E. and Sharma, A.K., Cytomixis in Pollen Mother Cells of an Apomictic Ornamental Ervatamia divaricata (Linn.) Alston, Cytologia, 1983, vol. 48, pp. 201–207.
Omara, M.K., Cytomixis in Lolium perenne, Chromosoma, 1976, vol. 55, pp. 267–271.
Risueno, M.C., Gimenez-Martin, G., Lopez-Saez, J.F., and R-Garcia, M.I., Connexions between Meiocytes in Plants, Cytologia, 1969, vol. 34, pp. 262–272.
Romanov, I.D. and Orlova, I.N., Cytomixis and Its Consequences in Microsporocytes of Triticale, Genetika, 1971, vol. 7, no. 12, pp. 5–13.
Shamina, N. V. and Sidorchuk, Yu. V., Dynamics of Micro tubular Cytoskeleton in Meiosis in the Higher Plants. X. The General Scheme of the Cytoskeleton Cycle, Tsitologiya, 2006, vol. 48, no. 5, pp. 427–437.
Shamina, N.V., Dorogova, N.V., Zagorskaya, A.A., Deineko, E.V., and Shumnyi, V.K., Disturbances of Male Meiosis in Tobacco Transgenic Line res91, Tsitologiya, 2000, vol. 42, no. 12, pp. 1173–1178.
Sheidai, M. and Bagheri-Shabestarei, E.S., Cytomixis and Unreduced Pollen Formation in Some Festuca L. Species of Iran, Caryologia, 2007, vol. 604, pp. 364–371.
Sheidai, M., Jaffari, S., Taleban, P., and Keshavarzi, M., Cytomixis and Unreduced Pollen Grain Formation in Alopecurus L. and Catbrosa Beauv. (Poaceae), Cytologia, 2009, vol. 74, pp. 31–41.
Shnaider, T., About the Issue of Cytomixis in Plants, Izv. AN Est. SSR, 1975, vol. 24, no. 3, pp. 199–209.
Singhal, V.K. and Kumar, P., Cytomixis during Microsporogenesis in the Diploid and Tetraploid Cytotypes of Withania somnifera L. Dunal 1852 (Solanaceae), Comp. Cytogenet., 2008, vol. 2, no. 1, pp. 85–92.
Singhal, V.K. and Kumar, P., Impact of Cytomixis on Meiosis, Pollen Viability and Pollen Size in Wild Populations of Himalayan Poppy (Meconopsis aculeate Royle), J. Biosci., 2008, vol. 33, no. 3, pp. 371–380.
Tarkowaska, J., Cytomixis in the Epidermis of Scales and Leaves and in Meristems of Root Apex of Allium cepa L., Acta Soc. Bot. Polon., 1960, vol. 34, pp. 27–44.
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Published in Russian in Ontogenez, 2012, Vol. 43, No. 4, pp. 261–267.
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Kumar, G., Yadav, R.S. Induction of cytomixis affects microsporogenesis in Sesamum indicum L. (Pedaliaceae). Russ J Dev Biol 43, 209–214 (2012). https://doi.org/10.1134/S1062360412040054
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DOI: https://doi.org/10.1134/S1062360412040054