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Effect of exogenous factors on the induction of spicule formation in sea urchin embryonic cell cultures

  • Cell Differentiation and Proliferation
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Abstract

The effect of exogenous factors on the realization of the spicule formation program in two sea urchin species, Strongylocentrotus intermedius and S. nudus, has been studied in primary embryonic cell cultures derived from the blastula and gastrula stages. It has been shown that the process of spicule formation depends on the type of substrate and the composition of the medium. An original finding is that calf or horse serum necessary for spicule formation in vitro can be replaced by a complex of factors including insulin, transferrin, and lectins. Methods allowing control over the growth and differentiation of marine invertebrate embryonic cells in vitro open prospects for their application to practical problems such as the establishment of cell cultures producing certain mineral structures.

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Authors and Affiliations

  1. Zhirmunsky Institute of Marine Biology, Far East Branch, Russian Academy of Sciences, ul. Pal’chevskogo 17, Vladivostok, 690059, Russia

    Yu. O. Kipryushina & N. A. Odintsova

  2. Far East Federal University, Vladivostok, 690600, Russia

    Yu. O. Kipryushina & N. A. Odintsova

Authors
  1. Yu. O. Kipryushina
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  2. N. A. Odintsova
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Correspondence to Yu. O. Kipryushina.

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Original Russian Text © Yu.O. Kipryushina, N.A. Odintsova, 2011, published in Ontogenez, 2011, Vol. 42, No. 5, pp. 390–396.

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Kipryushina, Y.O., Odintsova, N.A. Effect of exogenous factors on the induction of spicule formation in sea urchin embryonic cell cultures. Russ J Dev Biol 42, 342–348 (2011). https://doi.org/10.1134/S1062360411050080

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  • DOI: https://doi.org/10.1134/S1062360411050080

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