Abstract
The chitosan added to heparinized blood plasma in vitro at concentrations of 10–2, 10–1, 1, 5, and 10 mg/mL dose-dependent reduced fibrinolysis, the prothrombin time, platelet aggregation, and blood clotting and increased the activity of factor XIIIa. A decrease in fibrinolysis and plasmin activity and increased FXIIIa activity and platelet aggregation were also detected in vivo in a rat model with hypocoagulation caused by intravenous administration of high-molecular heparin (400 IU/kg of body mass), 30 minutes after injection of chitosan (10 mg/kg of body mass). At the same time, the fibrinogen concentration was restored to the normal levels. It was accompanied by an increase in the activity of the tissue plasminogen activator and an extension of the blood clotting time (APTT test). A conclusion on the multidirectional effect of chitosan on the hemostasis parameters under hypocoagulation was made, and the possible mechanisms of chitosan action were considered.
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ACKNOWLEDGMENTS
The authors are grateful to G.Yu. Lyapin who facilitated the work.
Funding
This work was carried out with the financial support of the Moscow State University on the state budgetary fundamental topic “Regulators of the coagulation and anticoagulation systems in normal and pathological conditions” (Cytis no. 121032300076-3).
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The idea of work and planning of the experiment: L.A. Lyapina and M.E. Grigorjeva; conducting experiments: all authors; data processing: all authors; article writing: L.A. Lyapina and M.E. Grigorjeva; article editing: T.Yu. Obergan.
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Lyapina, L.A., Obergan, T.Y., Grigorjeva, M.E. et al. The Effect of Chitosan on Blood Coagulation in Heparinized Rats. Biol Bull Russ Acad Sci 49, 666–670 (2022). https://doi.org/10.1134/S1062359022060097
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DOI: https://doi.org/10.1134/S1062359022060097