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The Regenerative Capacity of Siberian Larch Cell Lines In Vitro

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Abstract

The results of a study of the regenerative capacity of eleven cell lines (CLs) of Siberian larch cultivated in vitro on AI nutrient medium for one to ten years are presented. The CLs differed in the productivity and ploidy of embryogenic cultures, the morphogenesis of somatic embryos, and their ability to maturate and germinate. A high regenerative capacity was found in CL6. Embryogenic cultures of this cell line produced large globular embryos and had a high productivity of the embryonal-suspensor mass (ESM) and stable ploidy. Genotyping at nine nuclear microsatellite loci of cloned larch trees showed complete identity of their CL6, from which they were obtained. At the age of seven, generative organs were formed in tree clones. In the subsequent spring–summer period, the process of micro- and macrosporogenesis was completed and pollination and seed development took place.

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Funding

This work was supported in part by the Russian Foundation for Basic Research, the Government of the Krasnoyarsk Territory, and the Krasnoyarsk Regional Fund for Support of Scientific and Scientific and Technical Activities within the framework of scientific projects no. 19-44-240009 and RFBR-Bel_a no. 18-54-00010.

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  1. Sukachev Institute of Forests, Federal Research Center, Krasnoyarsk Science Center, Russian Academy of Sciences, Siberian Branch,”, 660036, Krasnoyarsk, Russia

    I. N. Tretyakova, M. E. Park & N. V. Oreshkova

  2. Genome Research and Education Center, Siberian Federal University, 660041, Krasnoyarsk, Russia

    N. V. Oreshkova

  3. Forest Research Institute of National Academy of Sciences of Belarus, 246050, Gomel, Republic of Belarus

    V. E. Padutov

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  1. I. N. Tretyakova
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Tretyakova, I.N., Park, M.E., Oreshkova, N.V. et al. The Regenerative Capacity of Siberian Larch Cell Lines In Vitro. Biol Bull Russ Acad Sci 49, 609–619 (2022). https://doi.org/10.1134/S1062359022050193

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