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Effect of Cryopreservation Time on the Activity of Ionotropic Glutamatergic Mechanisms: An in vitro Study

  • ANIMAL AND HUMAN PHYSIOLOGY
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Abstract

The effects of cryopreservation for different periods of time on the amplitudes of AMPA (α‑amino-3-hydroxy-5-methylisoxazole-4-propionic acid) and NMDA (N-methyl-D-aspartate) glutamatergic ionotropic mechanisms and the action potential of the lateral olfactory tract (AP LOT) at –20°C and subsequent warming to 37°C was studied. Experiments were performed on brain slices of 22 nonhibernating animals—Wistar rats. After cryopreservation, the glutamatergic ionotropic mechanisms were preserved and restored using electrophysiological recording of the AMPA and NMDA potentials and the total AP LOT. The AMPA-dependent mechanisms and the activity of conductive LOT fibers were restored to the normothermal values; the recovery of the NMDA-dependent mechanisms was incomplete and averaged 34% compared with the normothermal values; the long-term post-tetanic potentiation was not restored after cryopreservation.

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Funding

This study was supported under the Fundamental Scientific Research Program of State Academies for 2013–2020 (GP-14, Sect. 65.2).

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Authors and Affiliations

  1. Pavlov Institute of Physiology, Russian Academy of Sciences, 199034, St. Petersburg, Russia

    A. A. Mokrushin

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  1. A. A. Mokrushin
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Correspondence to A. A. Mokrushin.

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Conflict of interests. The authors declare that they have no conflict of interest.

Statement on the welfare of animals. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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Translated by N. Smolina

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Mokrushin, A.A. Effect of Cryopreservation Time on the Activity of Ionotropic Glutamatergic Mechanisms: An in vitro Study. Biol Bull Russ Acad Sci 47, 71–79 (2020). https://doi.org/10.1134/S1062359020010094

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  • DOI: https://doi.org/10.1134/S1062359020010094

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