Skip to main content
SpringerLink
Log in

Muscle regeneration and the state of the thymus in adult rats under laser irradiation and alloplasty of newborn gastrocnemius muscle and diaphragm

  • Developmental Biology
  • Published:
Biology Bulletin Aims and scope Submit manuscript

Abstract

The regeneration of adult rat gastrocnemius muscles has been studied under implantation of new-born rat muscles into area of muscle trauma. Alloplasty was performed using minced gastrocnemius and diaphragm muscles, which differs at birth in animals by degree of differentiation. The rat-recipient area of alloplasty was subjected to He-Ne laser radiation before operation, with the aim of reducing the immune response to allogenic muscle tissue. It has been shown that the number of regenerating myofibers produced from implanted gastrocnemius muscles is more than in alloplants from diaphragms. However, the formation of cartilage, bone, and adipose tissue foci was observed in the alloplastic region throughout the whole regeneration period. After implantation of minced diaphragm muscles, cartilage nodes were observed only in 7-day regenerates. At the end of observation, in the first instance, the area of muscle trauma in adult rat muscles was replaced by adipose tissue, even in the case of initial laser irradiation. After the implantation of diaphragm muscles, the area of trauma was filled with regenerating muscle tissue.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Abelev, G.I., Fundamentals of Immunity, Soros. Obshcheobrazovat. Zhurn/, 1996, no. 5, pp. 4–10.

  • Avtandilov, G.G., Yabluchanskii, N.I., and Gubenko, V.G., Sistemnaya stereometriya v izuchenii patologicheskogo protsessa (Systemica Stereometry in Studying Pathological Process), Moscow: Meditsina, 1981.

    Google Scholar 

  • Bischoff, R., The Satellite Cell and Muscle Regeneration, in Myogenesis, Engel, A.G. and Franszini-Armstrong, C., Eds., New York: McGraw-Hill, 1994, vol. 2, pp. 97–118.

    Google Scholar 

  • Bulyakova, N.V. and Azarova, V.S., Morphofunctional Characteristics of the Thymus and Muscle Regenerates under Laser Irradiation and Alloplasty of Adult Muscle Tissue in the Area of Trauma, Izv. Akad. Nauk, Ser. Biol., 2009, vol. 36, no. 1, pp. 18–26 [Biol. Bull (Eng. Transl.), 2009, vol. 36, no.1, pp. 12–19].

    Google Scholar 

  • Bulyakova, N.V., Popova, M.F., Zubkova, S.M., et al., Stimulyatsiya regeneratsii obluchennykh i neobluchennykh skeletnykh myshts mlekopitayushchikh. Lazernaya i tkanevaya terapiya (Stimulation of Regeneration of Irradiated and Nonirradiated Skeletal Muscles of Mammals. Laser and Tissue Therapy), Moscow: Nauka, 1995.

    Google Scholar 

  • Bulyakova, N.V., Stenina, M.A., Azarova, V.S., et al., Regenerative Activity of Muscle Tissue and Thymus Status in Young and Old mdx Mice with Muscle Injury and Wound Xenoplasty, Izv. Akad. Nauk, Ser. Biol., 2008, vol. 35, no. 2, pp. 174–185 [Biol. Bull (Eng. Transl.), 2008, vol. 35, no. 2, pp. 151–160].

    Google Scholar 

  • Charge, S.B.P. and Rudnicki, M.A., Cellular and Molecular Regulation of Muscle Regeneration, Physiol. Reviews, 2004, vol. 84, no. 1, pp. 209–238.

    Article  CAS  Google Scholar 

  • Danilov, R.K., Adaptive Mechanisms of Development of Somatic Muscle Tissue in Mammalian Ontogeny, in Morfoadaptatsiya myshts v norme i patologii (Morphological Adaptation of Muscles in Normal State and Pathology), Saratov: Izd. Sarat. Univ., 1975, pp. 36–47.

    Google Scholar 

  • Davis, H.L. and Jasmin, B.J., Direct Gene Transfer into Mouse Diaphragm, FEBS Lett., 1993, vol. 33, pp. 146–150.

    Article  Google Scholar 

  • Davis, H.L., Michel, M.L., Mancini, M., et al., Direct Gene Transfer in Skeletal Muscle: Application to Gene Therapy and Genetic Vaccination, Acta Cardiomiol., 1993, vol. 5, no. 2, pp. 173–179.

    Google Scholar 

  • Fedorchuk, A.G. and Skivka, L.M., Effect of Laser Irradiation of Blood on Some Immunity Indices in Mice, in Deistvie nizkoenergeticheskogo lazernogo izlucheniya na krov’ (Effect of Low-Power Laser Radiation on Blood), Kiev: Naukova Dumka, 1989, pp. 48–49.

    Google Scholar 

  • Geissinger, H.D., Bhatnagar, M.K., and Friesen, D.L., Comparison of Mitochondria between Adult and Neonate Rabbit Muscle: A Scanning and Transmission Electron Microscopic Study, Trans. Am. Microsc. Soc., 1984, vol. 103, no. 3, pp. 275–283.

    Article  Google Scholar 

  • Gottschall, J., The Diaphragm of the Rat and Its Innervations. Muscle Fiber Composition Pericaria and Axons of Efferent Neurons, Anat. Embriol., 1981, vol. 161, no. 4, pp. 405–419.

    Article  CAS  Google Scholar 

  • Hall, Z.W., Neuromuscular Junction Formation, in Muscle Development. Molecular and Cellular Control, New York: McGraw-Hill, 1982, pp. 455–458.

    Google Scholar 

  • Heslop, L., Beauchamp, J.R., Tajbakhsh, S., et al., Transplanted Primary Neonatal Myoblasts Can Give Rise to Functional Satellite Cells as Identified using the Myf5nlacZL+ Mouse, Gene Therapy, 2001, vol. 8, no. 10, pp. 778–783.

    Article  CAS  PubMed  Google Scholar 

  • Huard, J., Verreault, S., Roy, R., et al., High Efficiency of Muscle Regeneration after Human Myoblast Clone Transplantation in SCID Mice, J. Clin. Invest., 1994, vol. 93, pp. 586–599.

    Article  CAS  PubMed  Google Scholar 

  • Ito, H., Vilquin, J.T., Skuk, D., et al., Myoblast Transplantation in Non-Dystrophic Dog, Neuromuscul. Disord., 1998, vol. 8, no. 2, pp. 95–110.

    Article  CAS  PubMed  Google Scholar 

  • Karmolin, A.L. and Kalina, O.V., Effect of Laser Radiation with a Wavelength of 633 nm on Some Indices of the Immune Status of the Body, in Kliniko-gigienicheskie problemy v svyazi s razvitiem kvantovoi elektroniki (Clinical-Hygienic Problems in Relation to Developmment of Quantum Electronics), Moscow: Meditsina, 1982, pp. 111–115.

    Google Scholar 

  • Kharin, G.M., Changes in the Cytoarchitectonics of Lymphoid Organs after Exposure to Laser Radiation, in Primenenie lazerov v khirurgii i meditsine (The Use of Lasers in Surgery and Medicine), Moscow: VINITI, 1989, part 1, pp. 567–569.

    Google Scholar 

  • Lucas, P.A., Calcute, A.F., Southerland, S.S., et al., A Population of Cells Resident within Embryonic and Newborn Rat Skeletal Muscle Is Capable of Differentiating Into Multiple Mesodermal Phenotypes, Wound Repair Regenerat., 1995, vol. 3, pp. 449–459.

    Article  CAS  Google Scholar 

  • Mast, B.A. and Schultz, G.S., Interactions Cytokines, Growth Factors, and Proteases in Acute and Chronic Wounds, Wound Repair Regenerat., 1996, vol. 4, pp. 411–420.

    Article  CAS  Google Scholar 

  • Mester, E., Nagylucskay, S., Tisza, S., and Mester, A., Stimulation of Wound Healing by Means of Laser Rays: Investigation of the Effect on Immune Competent Cells, Acta Chir. Acad. Sci. Hung., 1978, vol. 19, no. 2, pp. 163–170.

    CAS  PubMed  Google Scholar 

  • Popova, M.F., Radiochuvstvitel’nost’ i stimuliruyushchie svoistva regeneriruyushchikh tkanei mlekopitayushchikh (Radiosensitivity and Stimulating Properties of Mammalian Regenerative Tissues), Moscow: Nauka, 1984.

    Google Scholar 

  • Puchkov, V.F., Smirnov, E.B., and Otellin, V.A., Rat Thymus Response to Xenotransplantation and Immunosuppression, Morfologiya, 1998, vol. 114, no. 4, pp. 59–64.

    CAS  Google Scholar 

  • Rogers, J.J., Young, H.E., Adkison, L.R., et al., Differentiation Factors Induce Expression of Muscle, Fat, Cartilage, and Bone in a Clone of Mouse Pluripotent Mesenchymal Stem Cells, Am. Surg., 1995, vol. 61, pp. 231–236.

    CAS  PubMed  Google Scholar 

  • Rumyantseva, O.N., The Role of Differentiation Level on Histogenesis in Transplanted Muscles, Zh. Obshch. Biol., 1965, vol. 26, no. 4, pp. 494–501.

    Google Scholar 

  • Studitskii, A.N. and Ignat’eva, Z.P., Vosstanovlenie myshts u vysshikh mlekopitayushchikh (Muscle Recovery in Higher Mammals), Moscow: Akad. Nauk SSSR, 1961.

    Google Scholar 

  • Sukhikh, G.T., Malaitsev, V.V., Bogdanova, I.M., et al., New Approach to Duchenne Muscular Dystrophy Treatment using Early Myogenesis Precursors, Byull. Eksp. Biol. Med., 2001, vol. 132, no. 12, pp. 604–612.

    Google Scholar 

  • Wang, L., Dobrzynski, E., Schlachterman, A., et al., Systemic Protein Delivery by Muscle-Gene Transfer Is Limited by a Local Immune Response, Blood, 2005, vol. 105, no. 11, pp. 4226–4234.

    Article  CAS  PubMed  Google Scholar 

  • Watt, D.J., Lambert, K., Morgan, J.E., et al., Incorporation of Donor Muscle Precursor Cells Into An Area of Muscle Regeneration in the Host Mouse, J. Neurol. Sci., 1982, vol. 57, pp. 319–331.

    Article  CAS  PubMed  Google Scholar 

  • Yamashiro, S., Harris, W.H., and Stopps, T.P., Ultrastructural Study of Developing Rabbit Diaphragm, J. Anat., 1984, vol. 139, no. 1, pp. 67–79.

    PubMed  Google Scholar 

  • Young, H.E., Mancini, M.L., Wright, R.P., et al., Mesenchymal Stem Cells Reside within the Connective Tissues of Many Organs, Am. J. Anat., 2005, vol. 202, no. 2, pp. 137–144.

    Article  Google Scholar 

  • Young, H.E., Wright, R.P., Mancini, M.L., et al., Bioactive Factors Affect Proliferation and Phenotypic Expression in Progenitor and Pluripotent Stem Cells, Wound Repair. Regenerat., 1998, vol. 6, pp. 65–75.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, Leninskii pr. 33, Moscow, 119071, Russia

    N. V. Bulyakova & V. S. Azarova

Authors
  1. N. V. Bulyakova
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. V. S. Azarova
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to N. V. Bulyakova.

Additional information

Original Russian Text © N.V. Bulyakova, V.S. Azarova, 2010, published in Izvestiya Akademii Nauk, Seriya Biologicheskaya, 2010, No. 5, pp. 535–546.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bulyakova, N.V., Azarova, V.S. Muscle regeneration and the state of the thymus in adult rats under laser irradiation and alloplasty of newborn gastrocnemius muscle and diaphragm. Biol Bull Russ Acad Sci 37, 453–463 (2010). https://doi.org/10.1134/S1062359010050031

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1062359010050031

Key words

Search

Navigation