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Application of Induced Bathochromic Shift for Trace Level Quantification of Formaldehyde in Some Antiviral and Anti-Microbial Drugs with Cinnamaldehyde as 2,4-Dinitrophenyl Hydrazine Scavenger Using UV-Visible Spectroscopy

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Abstract

A highly sensitive analytical method has been developed for trace level quantification of formaldehyde in active pharmaceutical ingredient. Formaldehyde is a highly toxic impurity. Determination of formaldehyde by using UV-visible spectroscopy is difficult due to interference of unreacted derivatizing agent; hence, either HPLC-UV or GC-MS are used for the quantification. However, we have developed an indirect method for the quantification of formaldehyde. In this method, in the first step, formaldehyde reacted with excess of 2,4-dinitrophenyl hydrazine (2,4-DNPH). In the second step, the quantification of unreacted 2,4-DNPH was done by detecting its derivative with its scavenger—cinnamaldehyde on a UV-visible spectrophotometer. The developed method is rapid, simple, sensitive and accurate for the quantification of formaldehyde. Linearity of the method was observed in the range of 0.5–3 μg/mL with determination coefficients (r2) > 0.999. The limit of detection and limit of quantification are 0.25 and 0.5 μg/mL, respectively.

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Authors and Affiliations

  1. Department of Chemistry, N.G. Acharya and D.K. Marathe College, Chembur, 400071, Mumbai, India

    Vidyagauri V. Lele &  Uttam Prabhanand Dalvi

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  1. Vidyagauri V. Lele
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  2. Uttam Prabhanand Dalvi
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Correspondence to Vidyagauri V. Lele or Uttam Prabhanand Dalvi.

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Vidyagauri V. Lele, Uttam Prabhanand Dalvi Application of Induced Bathochromic Shift for Trace Level Quantification of Formaldehyde in Some Antiviral and Anti-Microbial Drugs with Cinnamaldehyde as 2,4-Dinitrophenyl Hydrazine Scavenger Using UV-Visible Spectroscopy. J Anal Chem 78, 681–686 (2023). https://doi.org/10.1134/S1061934823060072

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