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Determination of the Origin of 19-Norandrosterone in Urine by Gas Chromatography–Isotope-Ratio Mass Spectrometry for Doping Control

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Abstract

The most difficult task in antidoping control is the identification of compounds whose origin can be either endogenous or exogenous. This paper presents a procedure for determining the origin of 19-norandrosterone based on the extraction of target components from urine, their multistage purification by semipreparative HPLC, acetylation, and quantification by gas chromatography–isotope-ratio mass spectrometry and gas chromatography–mass spectrometry. Effects of isotope fractionation were not observed in sample preparation. The degree of extraction of the target analytes was 80 ± 5%.

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Authors and Affiliations

  1. Antidoping Centre, Moscow, 105005, Russia

    I. I. Podolskiy & M. A. Dikunets

  2. UCLA Olympic Analytical Laboratory, Los Angeles, California, CA, 90025, USA

    T. G. Sobolevskii

Authors
  1. I. I. Podolskiy
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  2. T. G. Sobolevskii
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  3. M. A. Dikunets
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Correspondence to I. I. Podolskiy.

Additional information

Original Russian Text © I.I. Podolskiy, T.G. Sobolevskii, M.A. Dikunets, 2018, published in Zhurnal Analiticheskoi Khimii, 2018, Vol. 73, No. 3, pp. 224–234.

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Podolskiy, I.I., Sobolevskii, T.G. & Dikunets, M.A. Determination of the Origin of 19-Norandrosterone in Urine by Gas Chromatography–Isotope-Ratio Mass Spectrometry for Doping Control. J Anal Chem 73, 283–291 (2018). https://doi.org/10.1134/S1061934818010082

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  • DOI: https://doi.org/10.1134/S1061934818010082

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