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A Novel Molecular Imprint Polymer Quartz Crystal Microbalance Nanosensor for the Detection of Andrographolide in the Medicinal Plant Extract

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Abstract

A novel quartz crystal microbalance (QCM) sensor based on molecular imprint polymer (MIP) has been developed for the detection of andrographolide in the medicinal plant extracts. The MIP and non-imprinted polymer (NIP) were synthesized using a precipitation polymerization method. The polymers were characterized by Fourier-transform infrared spectrometry (FTIR), scanning electron microscopy (SEM) and cyclic voltammetry. Both MIP and NIP were deposited on quartz crystal platform using an electro-polymerization method. A linear Langmuir isotherm model revealed that the MIP-QCM sensor has the maximum binding sites 18.02 ng cm–2. It was found that 92.8% of andrographolide was detected in 0.10 μg mL–1 of plant extract, and the limits of detection and quantification values were 1.21 and 4.02 ng cm–2, respectively. This research showed that the andrographolide imprinted polymer could be used to detect and quantify andrographolide in Andrographis paniculata plant extract in both analytical separation and sensor development.

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Funding

The authors are grateful to Universiti Sains Malaysia for providing the financial support of this project Bridging grant no. 304.PKIMIA.6316279 and Research Management Centre, IIUM grant no. RPDF19 002 0012.

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Authors and Affiliations

  1. School of Chemical Sciences, Universiti Sains Malaysia, 11800, Minden, Pulau Pinang, Malaysia

    A. K. M. Shafiqul Islam & Mohd Noor Ahmad

  2. Department of Chemical Engineering Technology, Universiti Malaysia Perlis, Uniciti Alam Campus Sungai Chuchuh, 02100, Padang Besar, Perlis, Malaysia

    Hemavathi Krishnan

  3. Centre of Diploma Studies, Universiti Malaysia Perlis, Uniciti Alam Campus, Sungai Chuchuh, 02100, Padang Besar, Perlis, Malaysia

    Pubalan Nadaraja

  4. Analytical and Bioanalytical Research Laboratory, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, International Islamic University Malaysia, 25200, Kuantan, Pahang, Malaysia

    A. B. M. Helal Uddin

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  1. A. K. M. Shafiqul Islam
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Correspondence to A. K. M. Shafiqul Islam.

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Shafiqul Islam, A.K., Krishnan, H., Ahmad, M.N. et al. A Novel Molecular Imprint Polymer Quartz Crystal Microbalance Nanosensor for the Detection of Andrographolide in the Medicinal Plant Extract. Russ J Electrochem 57, 671–679 (2021). https://doi.org/10.1134/S1023193520120228

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