Skip to main content
SpringerLink
Log in

Electrochemical DNA impedance biosensor for the detection of DNA hybridization with polymeric film, single walled carbon nanotubes modified glassy carbon electrode

  • Published:
Russian Journal of Electrochemistry Aims and scope Submit manuscript

Abstract

In this work, we report the fabrication of a sensitive electrochemical DNA impedance biosensor for the detection of sequence-specific target DNA. p-Aminobenzoic acid was first immobilized on the surface of the electrode modified with single walled carbon nanotubes with carboxylic acid groups (SWCNTs) by cyclic voltammetry (CV). A single-stranded DNA probe with a NH2 group at the end (H2N-ssDNA) was then covalently immobilized on the surface of polymeric film at room temperature. The impedance measurement was performed in a solution containing 5 mM K3[Fe(CN)6]/K4[Fe(CN)6]. The change of interfacial charge transfer resistance (R CT) was confirmed the hybrid formation. The difference of R CT was linear with the logarithm of complementary oligonucleotides concentrations in the range of 1.0 × 10−12 to 1.0 × 10−7 M, with a detection limit of 3.5 × 10−13 M (S/N = 3).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Staudt, L.M., Trends Immunol., 2001, vol. 22, p. 35.

    Article  CAS  Google Scholar 

  2. Palecek, E. and Jelen, F., Crit. Rev. Anal. Chem., 2002, vol. 32, p. 261.

    Article  CAS  Google Scholar 

  3. Castañeda, M.T., Merkoçi, A., Pumera, M., and Alegret, S., Biosens. Bioelectron., 2007, vol. 22, p. 1961.

    Article  Google Scholar 

  4. Fang, X.H., Liu, X.J., Schuster, S., and Tan, W.H., J. Am. Chem. Soc., 1999, vol. 121, p. 2921.

    Article  CAS  Google Scholar 

  5. Zhi, Z.L., Morita, Y., Yamamura, S., and Tamiya, E., Chem. Commun., 2005, vol. 19, p. 2448.

    Article  Google Scholar 

  6. Lucarelli, F., Tombelli, S., Minunni, M., Marrazza, G., and Mascini, M., Anal. Chim. Acta, 2008, vol. 609, p. 139.

    Article  CAS  Google Scholar 

  7. Kang, J.W., Li, X.N., Wu, G.F., Wang, Z.H., and Lu, X.Q., Anal. Biochem., 2007, vol. 364, p. 165.

    Article  CAS  Google Scholar 

  8. Wang, J., Polsky, R., and Xu, D., Langmuir, 2001, vol. 17, p. 5739.

    Article  CAS  Google Scholar 

  9. Li, M.Y., Huang, S.S., Zhu, P.S., Kong, L.M., Peng, B., and Gao, H., Electrochim. Acta, 2009, vol. 54, p. 2284.

    Article  CAS  Google Scholar 

  10. Bonanni, A., Esplandiu, M.J., and del Valle, M., Electrochimica Acta, 2008, vol. 53, p. 4022.

    Article  CAS  Google Scholar 

  11. Pan, S.L., and Rothberg, L., Langmuir 2005, vol. 21, p. 1022.

    Article  CAS  Google Scholar 

  12. Peng, H., Soeller, C., Vigar, N.A., Caprio, V., and Travas-Sejdic, J., Biosens. Bioelectron., 2007, vol. 22, p. 1868.

    Article  CAS  Google Scholar 

  13. Xu, Y., Jiang, Y., Cai, H., He, P.G., and Fang, Y.Z., Anal. Chim. Acta., 2004, vol. 516, p. 19.

    Article  CAS  Google Scholar 

  14. Huang, H., Liu, Z., and Yang, X., Anal. Biochem., 2006, vol. 356, p. 208.

    Article  CAS  Google Scholar 

  15. Tlili, C., Korri-Youssoufi, H., Ponsonnet, L., Martelet, C., and Jaffrezic-Renault, N.J., Talanta, 2005, vol. 68, p. 131.

    Article  CAS  Google Scholar 

  16. Patolsky, F., Filanovsky, B., Katz, E., and Willner, I., J. Phys. Chem. B, 1998, vol. 102, p. 10359.

    Article  CAS  Google Scholar 

  17. Wei, F., Sun, B., Guo, Y., and Zhao, X.S., Biosens. Bioelectron., 2003, vol. 18, p. 1157.

    Article  CAS  Google Scholar 

  18. Xu, Y., Cai, H., He, P. G., and Fang, Y.Z., Electroanalysis, 2004, vol. 16, p. 150.

    Article  CAS  Google Scholar 

  19. Cai, H., Xu, Y., He, P.G., and Fang, Y.Z., Electroanalysis, 2003, vol. 15, p. 23.

    Article  Google Scholar 

  20. Ma, K.S., Zhou, H., Zoval, J., and Madou, M., Sens and Act B, 2006, vol. 114, p. 58.

    Article  Google Scholar 

  21. Xu, Y., Ye, X.Y., Yang, L., He, P.G., and Fang, Y.Z., Electroanalysis, 2006, vol. 18, p. 1471.

    Article  CAS  Google Scholar 

  22. Zhang, K.Y., Ma, H.Y., Zhang, L.P., and Zhang, Y.Z., Electroanalysis, 2008, vol. 20, p. 2127.

    Article  CAS  Google Scholar 

  23. Zhang, Y.Z., Wang, J., and Xu, M.L., Colloids Surf. B, 2010, vol. 75, p. 179.

    Article  CAS  Google Scholar 

  24. Gu, H.R., Su, X.D., and Loh, K.P., Chem. Phy. Lett., 2004, vol. 388, p. 483.

    Article  CAS  Google Scholar 

  25. Subramanian, A., Irudayaraj, J., and Ryan, T., Biosens. Bioelectron., 2006, vol. 21, p. 998.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. College of Chemistry and Materials Science, Anhui Key Laboratory of Chem-Biosensing, Wuhu, 241000, P.R. China

    M. Wang, Q. Meng & Y. Zhang

  2. Hennan Institute of Science and Technology, Xinxiang, 453003, P.R. China

    W. Gong

Authors
  1. M. Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. W. Gong
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Q. Meng
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Y. Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Y. Zhang.

Additional information

Published in Russian in Elektrokhimiya, 2011, Vol. 47, No. 12, pp. 1464–1470.

The article is published in the original.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wang, M., Gong, W., Meng, Q. et al. Electrochemical DNA impedance biosensor for the detection of DNA hybridization with polymeric film, single walled carbon nanotubes modified glassy carbon electrode. Russ J Electrochem 47, 1368–1373 (2011). https://doi.org/10.1134/S1023193511120123

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1023193511120123

Keywords

Search

Navigation