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Association between BMP15 Gene Polymorphisms of Growth Traits and Litter Size in Qinghai Bamei Pigs

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Abstract

Some mutations in BMP15 gene (Bone morphogenetic protein 15) have been known to be associated with prolificacy in various breeds, which can regulate follicular development, granulosa cell proliferation, and cumulus differentiation, etc., and plays a crucial role in the regulation of female animal fertility. Polymorphism of BMP15 gene and its reproductive traits of Bamei pigs was studied by using DNA sequencing. Bioinformatics tools were used to find QTLs that affected the reproductive traits of Bamei pigs, so as to provide a basis for molecular marker-assisted selection. Which Bamei pigs were served as research objects, the polymorphic locus of BMP15 gene were detected by using DNA direct sequencing and analyze genotype. Sus scrofa BMP15 protein has a lot of similar structures with human and other mammals in the primary, secondary, and tertiary structures; and closely related to cattle in terms of genetic evolution. There were three SNPs sites found in BMP15 gene of the test group, which g.5969C > T, g.6543C > T, g.6653A > G, the results of Chi-square test indicated that three SNPs loci fitted in Hardy–Weinberg equilibrium (p > 0.05). Type1 and Type3 mutations were beneficial to increasing the litter size in Qinghai Bamei pigs, and the litter size by homozygous TT of Type1 mutation was significantly higher than wild homozygous CC and heterozygous CT (P < 0.05). The results of the present study indicated the potential of the observed SNP of BMP15 for further exploitation in marker-assisted selection to improve reproduction efficiency of Bamei pigs.

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ACKNOWLEDGMENTS

The authors are grateful to the staff at the original breeding farm of the Huzhu Bamei pigs in Qinghai for collecting samples.

Funding

This work was supported by the Scientific and Technological Achievements Transformation Project of Qinghai Provincial Department of Science and Technology (2019-NK-102).

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Authors and Affiliations

  1. Plateau Livestock Genetic Resources Protection and Innovative Utilization Key Laboratory of Qinghai Province, Qinghai University Academy of Animal and Veterinary Sciences, 810016, Xining, Qinghai, China

    W. Shen, L. Wang, Yu. Ma, Ya. Cao, Q. Han, S. Wu & G. Wu

  2. Qinghai Provincial Center for Animal Disease Control and Prevention, 810099, Xining, Qinghai, China

    X. Zhang

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  1. W. Shen
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Correspondence to G. Wu.

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Conflict of interest. The authors declare that they have no conflicts of interest.

Statement on the welfare of animals. All experimental procedures were approved by the Review Committee for the Use of Qinghai University. Animal experimentation, including sample collection, was performed in agreement with the ethics commission’s guidelines. The use of experimental animals was permitted by local animal welfare laws, guidelines, and policies.

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Shen, W., Wang, L., Ma, Y. et al. Association between BMP15 Gene Polymorphisms of Growth Traits and Litter Size in Qinghai Bamei Pigs. Russ J Genet 58, 997–1006 (2022). https://doi.org/10.1134/S1022795422080075

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