Skip to main content
SpringerLink
Log in

Application of SRAP Markers for DNA Identification of Russian Alfalfa Cultivars

  • PLANT GENETICS
  • Published:
Russian Journal of Genetics Aims and scope Submit manuscript

Abstract

This study was carried out to estimate the genetic diversity between cultivars of alfalfa using SRAP marker system (sequence-related amplified polymorphism). We tested 25 combinations of SRAP markers and revealed that seven of them were informative for DNA polymorphism identification. These combinations generated 129 PCR products with the percentage of polymorphic bands per pair ranging from 21 to 50%. Specific amplification products were identified for 14 cultivars for genetic certification.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1.
Fig. 2.
Fig. 3.

Similar content being viewed by others

REFERENCES

  1. Piskovatskii, Yu.M., Kosolapov, V.M., Mikhalev, V.E., et al., Agrotekhnika vozdelyvaniya sortov lyutserny selektsii VNII kormov im. V.R. Vil’yamsa na semennye i kormovye tseli (Agronomic Technics for Cultivation of Alfalfa Cultivars Bred by the All-Russian Williams Federal Science Center for Fodder Production and Agroecology for Seed and Forage Purpose), Moscow: Rossiyskii Tsentr Sel’skokhozyaistvennogo Konsul’tirovaniya, 2008, pp. 3—15.

  2. Veronesi, F., Brummer, E.C., and Huyghe, C., Alfalfa, in Fodder Crops and Amenity Grasses, New York: Springer-Verlag, 2010, pp. 395—437.

    Google Scholar 

  3. Chesnokov, Yu.V. and Kosolapov, V.M., Geneticheskie resursy rastenii i uskorenie selektsionnogo protsessa (Plant Genetic Resources and Acceleration of the Breeding Process), Moscow: Ugreshskaya Tipografiya, 2016.

  4. Piskovatskii, Yu.M., Lomova, M.G., Solozhentseva, L.F., et al., Creation of promising alfalfa material with high seed productivity, Nauchnoe obespechenie kormoproizvodstva i ego rol’ v sel’skom khozyaistve, ekonomike, ekologii i ratsional’nom prirodopol’zovanii Rossii (Scientific Support of Fodder Production and its Role in Agriculture, Economy, Ecology and Rational Use of Natural Resources in Russia) (Proc. Int. Theor. Pract. Conf., in Memoriam of Acad. A.A. Zhuchenko, Lobnya, June 19—20, 2013), Moscow: Ugreshskaya Tipografiya, 2013, pp. 97—103.

  5. Khlestkina, E.K., Molecular markers in genetic studies and breeding, Russ. J. Genet.: Appl. Res., 2013, vol. 4, no. 3, pp. 236—244.

    Article  Google Scholar 

  6. Khlestkina, E.K., Molecular methods for analyzing the structure—function organization of genes and genomes in higher plants, Russ. J. Genet.: Appl. Res., 2011, vol. 15, pp. 757—768.

    Google Scholar 

  7. Li, G. and Quiros, C.F., Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica, Theor. Appl. Genet., 2001, vol. 103, nos. 2—3, pp. 455—461. https://doi.org/10.1007/s001220100570

    Article  CAS  Google Scholar 

  8. Osman, G., Suleyman, K., and Kazim, A., Diversity and relationships among Turkish okra germplasm by SRAP and phenotypic marker polymorphism, Biologia (Bratislava), 2007, vol. 62, no. 1, pp. 41—45. https://doi.org/10.2478/s11756-007-0010-y

    Article  CAS  Google Scholar 

  9. Lin, Z., Zhang, X., Nie, Y., et al., Construction of a genetic linkage map for cotton based on SRAP, Chin. Sci. Bull., 2003, vol. 48, no. 19, pp. 2064—2068. https://doi.org/10.1360/03wc0193

    Article  CAS  Google Scholar 

  10. Luo, Y.X., Du, D.Z., Fu, G., et al., Inheritance of leaf color and sequence-related amplified polymorphic (SRAP) molecular markers linked to the leaf color gene in Brassica juncea, Afr. J. Biotechnol., 2011, vol. 10, no. 66, pp. 14724—14730. https://doi.org/10.5897/AJB11.1096

    Article  CAS  Google Scholar 

  11. Hao, Q., Liu, Z.A., Shu, Q.Y., et al., Studies on Paeonia cultivars and hybrids identification based on SRAP analysis, Hereditas, 2008, vol. 145, no. 1, pp. 38—47. https://doi.org/10.1111/j.0018-0661.2008.2013.x

    Article  PubMed  Google Scholar 

  12. Guo, D.L. and Luo, Z.R., Genetic relationships of some PCNA persimmons (Diospyros kaki Thumb.) from China and Japan revealed by SRAP analysis, Genet. Resour. Crop. Evol., 2006, vol. 53, no. 8, pp. 1597—1603. https://doi.org/10.1007/s10722-005-8717-5

    Article  CAS  Google Scholar 

  13. Dellaporta, S.L., Wood, J., and Hicks, J.B., A plant DNA minipreparation: version II, Plant Mol. Biol. Rep., 1983, vol. 1, no. 4, pp. 19—21.

    Article  CAS  Google Scholar 

  14. Robarts, D.W.H. and Wolfe, A.D., Sequence-related amplified polymorphism (SRAP) markers: a potential resource for studies in plant molecular biology, Appl. Plant Sci., 2014, vol. 2, no. 7, p. 1400017. https://doi.org/10.3732/apps.1400017

    Article  Google Scholar 

  15. Aneja, B., Yadav, N.R., Chawla, V., and Yadav, R.C., Sequence-related amplified polymorphism (SRAP) molecular marker system and its applications in crop improvement, Mol. Breed., 2012, vol. 30, no. 4, pp. 1635—1648. https://doi.org/10.1007/s11032-012-9747-2

    Article  CAS  Google Scholar 

  16. Vandemark, J.G., Ariss, J.J. Bauchan, G.A., et al., Estimating genetic relationships among historical sources of alfalfa germplasm and selected cultivars with sequence related amplified polymorphisms, Euphytica, 2006, vol. 152, no. 1, pp. 9—16. https://doi.org/10.1007/s10681-006-9167-7

    Article  CAS  Google Scholar 

  17. Rhouma, H.B., Taski-Ajdukovic, K., Zitouna, N., et al., Assessment of the genetic variation in alfalfa genotypes using SRAP markers for breeding purposes, Chil. J. Agric. Res., 2017, vol. 77, no. 4, pp. 332—339. https://doi.org/10.4067/S0718-58392017000400332

    Article  Google Scholar 

  18. Al-Faifi, S., Migdadi, H., Al-Doss, A., et al., Morphological and molecular genetic variability analyses of Saudi lucerne (Medicago sativa L.), Crop Pasture Sci., 2013, vol. 64, no. 2, pp. 137—146. https://doi.org/10.1071/CP12271

    Article  CAS  Google Scholar 

  19. Talebi, M.B., Hajiahmadi, Z., and Rahimmalek, M., Genetic diversity and population structure of four Iranian alfalfa populations revealed by sequence-related amplified polymorphism (SRAP) markers, J. Crop. Sci. Biotech., 2011, vol. 14, no. 3, pp. 173—178. https://doi.org/10.1007/s12892-011-0030-6

    Article  Google Scholar 

  20. Yuan, Q., Gao, J., Gui, Z., et al., Genetic relationships among alfalfa germplasms resistant to common leaf spot and selected Chinese cultivars assessed by sequence-related amplified polymorphism (SRAP) markers, Afr. J. Biotech., 2011, vol. 10, no. 59, pp. 12527—12534. https://doi.org/10.5897/AJB11.901

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Federal Williams Research Center of Forage Production and Agroecology (FWRC FPA), 141055, Lobnya, Moscow oblast, Russia

    A. O. Shamustakimova, Y. M. Mavlyutov & I. A. Klimenko

Authors
  1. A. O. Shamustakimova
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Y. M. Mavlyutov
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. I. A. Klimenko
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Y. M. Mavlyutov.

Ethics declarations

The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Shamustakimova, A.O., Mavlyutov, Y.M. & Klimenko, I.A. Application of SRAP Markers for DNA Identification of Russian Alfalfa Cultivars. Russ J Genet 57, 540–547 (2021). https://doi.org/10.1134/S1022795421050112

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1022795421050112

Keywords:

Search

Navigation