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Peculiarities of the SprIR Quorum Sensing System of Serratia proteamaculans 94 and Its Involvement in Regulation of Cellular Processes

  • GENETICS OF MICROORGANISMS
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Abstract

The quorum sensing (QS) system SprIR of the psychrotrophic strain Serratia proteamaculans 94 was investigated. A mutant was constructed with the inactivated sprR gene encoding the regulatory receptor protein SprR. Inactivation of this gene was shown to affect the composition of fatty acids synthesized by S. proteamaculans 94 and did not affect the synthesis of N-acyl-L-homoserine-lactones (AHL); the activities of extracellular proteases, chitinases, and hemolysins; the swimming motility of cells; and the suppression of mycelium growth of fungal plant pathogens by volatile compounds emitted by this strain. Inactivation of the sprI gene (but not the sprR gene) reduced the biofilm formation, which increased when exogenous AHL was added to the culture. The comparative proteomic analysis of cell of the parent strain and mutant strains with inactivated sprI and sprR genes showed that the expression of 30 proteins in S. proteamaculans 94 is affected by the SprIR quorum sensing system.

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Funding

This work was partially supported by the Russian Foundation for Basic Research, projects no. 18-04-00375 and no. 19-04-00756, and by a grant of the Ministry of Education and Sciences of the Russian Federation, State Order for Yaroslavl State University, project no. 0856-2020-0008.

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Authors and Affiliations

  1. Institute of Molecular Genetics, National Research Center Kurchatov Institute, 123182, Moscow, Russia

    Yu. V. Zaitseva, V. A. Lipasova, O. A. Koksharova, V. A. Plyuta, I. V. Demidyuk & I. A. Khmel

  2. Demidov Yaroslavl State University, 150003, Yaroslavl, Russia

    Yu. V. Zaitseva

  3. Belozersky Institute of Physical-Chemical Biology, Moscow State University, 119899, Moscow, Russia

    O. A. Koksharova

  4. Department of Plant Pathology and Microbiology, Hebrew University of Jerusalem, 76100, Rehovot, Israel

    L. S. Chernin

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  1. Yu. V. Zaitseva
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Correspondence to I. A. Khmel.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Translated by E. Makeeva

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Zaitseva, Y.V., Lipasova, V.A., Koksharova, O.A. et al. Peculiarities of the SprIR Quorum Sensing System of Serratia proteamaculans 94 and Its Involvement in Regulation of Cellular Processes. Russ J Genet 57, 161–172 (2021). https://doi.org/10.1134/S1022795421020149

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