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Expression Features of the Transcription Factor Gene anthocyanin2 and Its Effect on the Anthocyanin Content in Capsicum chinense Jacq. Cultivars with Different Fruit Coloration

  • PLANT GENETICS
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Abstract

The homologous anthocyanin2 (AN2) genes were identified and structurally characterized in two cultivars of hot pepper Capsicum chinense with different fruit exocarp coloration (purple and yellow). In the analyzed cultivars, the anthocyanin content and the expression pattern of the CcAN2 gene and anthocyanin pathway structural genes DFR and UFGT, regulated by AN2, were determined in the vegetative and reproductive organs, including fruit tissues at different stages of its development. A positive correlation between transcription levels of CcAN2, CcDFR, and CcUFGT was shown. A direct link between transcription levels of the analyzed genes and the anthocyanin content was observed in fruit peel, but not in the leaves. It was assumed that, in the vegetative tissues of the studied cultivars, the AN2, DFR, and UFGT genes can participate in plant photoprotection, and the visible purple color due to the anthocyanin accumulation may be associated with the regulatory activity of other MBW genes.

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Funding

This work was supported by the Russian Science Foundation, project no. 19-16-00016, and partially by the Ministry of Science and Higher Education of the Russian Federation (M.A. Filyushin, phylogenetic analysis of the AN2 sequences of Solanaceae), and was performed using the experimental climate control facility in the Institute of Bioengineering, Research Centre of Biotechnology, Russian Academy of Sciences.

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Authors and Affiliations

  1. Federal Research Center Fundamentals of Biotechnology, Russian Academy of Sciences, 119071, Moscow, Russia

    M. A. Filyushin, A. V. Shchennikova & E. Z. Kochieva

  2. Federal Scientific Vegetable Center, 143080, VNIISSOK village, Moscow oblast, Russia

    M. A. Filyushin & E. A. Dzhos

  3. Moscow State University, 119991, Moscow, Russia

    E. Z. Kochieva

Authors
  1. M. A. Filyushin
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  2. E. A. Dzhos
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  3. A. V. Shchennikova
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  4. E. Z. Kochieva
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Correspondence to M. A. Filyushin.

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Translated by M. Bibov

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Filyushin, M.A., Dzhos, E.A., Shchennikova, A.V. et al. Expression Features of the Transcription Factor Gene anthocyanin2 and Its Effect on the Anthocyanin Content in Capsicum chinense Jacq. Cultivars with Different Fruit Coloration. Russ J Genet 56, 1203–1211 (2020). https://doi.org/10.1134/S1022795420090069

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