Abstract
The paper presents the results of the assessment of the frequency of the peripheral blood Т lymphocytes with micronuclei in Techa riverside residents who were chronically exposed in the 1950s. The study was performed 40–60 years after the onset of exposure. The exposed persons consisted of two groups: individuals who were first exposed in utero and then postnatally, and individuals who had only postnatal exposure. Cumulative dose to RBM in exposed persons varied within the range 0.001–4 Gy. A comparison group was also formed. It included individuals comparable in age, sex, and living conditions, but these people were not affected by accidental exposure. Findings of the study demonstrated that the frequency of lymphocytes with micronuclei was significantly higher in exposed women as compared to exposed men. The frequency of lymphocytes with micronuclei was significantly lower in those exposed in utero relative to the postnatally exposed persons and members of the comparison group. This decrease was observed both in women and in men. The study of the contribution of the cumulative dose to RBM revealed an increase in frequency of the lymphocytes with micronuclei in women exposed at doses of 0.1–0.49 Gy.
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REFERENCES
Preston, D.L., Cullings, H., Suyama, A., Funamoto, S., et al., Solid cancer incidence in atomic bomb survivors exposed in utero or as young children, J. Natl. Cancer Inst., 2008, vol. 100, no. 6, pp. 428—436. https://doi.org/10.1093/jnci/djn045
Boice, J.D., Jr. and Miller, R.W., Childhood and adult cancer after intrauterine exposure to ionizing radiation, Teratology, 1999, vol. 59, no. 4, pp. 227—233.
Gorbunova, V. and Seluanov, A., DNA double strand break repair, aging and the chromatin connection, Mutat. Res., Genet. Toxicol. Environ. Mutagen., 2016, vol. 788, pp. 2—6. https://doi.org/10.1016/j.mrfmmm.2016.02.004
Mediko-biologicheskie i ekologicheskie posledstviya radioaktivnogo zagryazneniya reki Techa (Medical, Biological, and Environmental Consequences of Radioactive Pollution of the Techa River), Akleev, A.V. and Kiselev, M.F., Eds., Moscow: Medbioekstrem, 2001.
Pastukhova, E.I., Shalaginov, S.A., and Akleev, A.V., The frequency of multiple pregnancy among the population of radioactively contaminated regions in the Chelyabinsk oblast, Vopr. Radiats. Bezop., 2011, no. 4(64), pp. 45—53.
Vozilova, A.V., Shagina, N.B., Degteva, M.O., and Akleyev, A.V., Chronic radioisotope effects on residents of the Techa River (Russia) region: cytogenetic analysis more than 50 years after onset of exposure, Mutat. Res., Genet. Toxicol. Environ. Mutagen., 2013, vol. 756, nos. 1—2, pp. 115—118. https://doi.org/10.1016/j.mrgentox.2013.05.016
Akleev, A.V., Veremeeva, G.A., and Kyoizumi, S., Long-term effects of chronic radiation exposure on the level of somatic mutations in peripheral blood cells, Radiats. Biol., Radioekol., 1998, vol. 38, no. 4, pp. 573—585.
Akleev, A., V., Krestinina, L.Yu., Preston, D., Devis, F., et al., Radiation risk of malignant neoplasms in residents of the riverside villages of the Tech River, Med. Radiol. Radiats. Bezop., 2008, vol. 53, no. 4, pp. 13—37.
Krestinina, L.Yu., Kharyuzov, Yu.E., Epiphanova, S.B., Tolstykh, E.I., et al., Cancer incidence after in utero exposure to ionizing radiation in Techa river residents, Radiat. Res., 2017, vol. 188, no. 3, pp. 314—324. https://doi.org/10.1667/RR14695.1
Posledstviya radioaktivnogo zagryazneniya reki Techi (Consequences of Radioactive Pollution of the Techa River), Akleev, A.V., Ed., Chelyabinsk: Kniga, 2016.
Fenech, M., The cytokinesis-block micronucleus technique and its application to genotoxicity studies in human populations, Environ. Health Perspect., 1993, no. 3, pp. 101—107. https://doi.org/10.1289/ehp.93101s3101
Degteva, M.O., Shagina, N.B., Vorob’eva, M.I., et al., Contemporary view on radioactive pollution of the Techa River in 1949—1956, Radiats. Biol., Radioekol., 2016, vol. 56, no. 5, pp. 523—534. https://doi.org/10.7868/S0869803116050039
Akhmadullina, Yu.R., Radiosensitivity of peripheral blood T-lymphocytes in first-generation offspring whose fathers were exposed to chronic radiation, Cand. Sci. (Biol.) Dissertation, Moscow, 2014.
Fenech, M., Chang, W.P., Kirsch-Volders, M., Holland, N., et al., HUMN project: detailed description of the scoring criteria for the cytokinesis-block micronucleus assay using isolated human lymphocyte cultures, Mutat. Res., 2003, vol. 534, nos. 1—2, pp. 65—75. https://doi.org/10.1016/S1383-5718(02)00249-8
Glantz, S.A., Primer of Biostatistics, New York: McGraw—Hill, 1997, 4th ed.
Akleev, A.V., Aleshchenko, A.V., Gotlib, V.Ya., et al., Adaptive response of blood lymphocytes of the inhabitants of the South Ural chronically exposed to radiation, Radiats. Biol., Radioekol., 2004, vol. 44, no. 4, pp. 426—431.
Fenech, M. and Bonassi, S., The effect of age, gender, diet and lifestyle on DNA damage measured using micronucleus frequency in human peripheral blood lymphocytes, Mutagenesis, 2011, vol. 26, no. 1, pp. 43—49. https://doi.org/10.1093/mutage/geq050
Tucker, J.D., Nath, J., and Hando, J.C., Activation status of the X chromosome in human micronucleated lymphocytes, Hum. Genet., 1996, no. 4, pp. 471—475.
Norppa, H. and Falck, G.C., What do human micronuclei contain?, Mutagenesis, 2003, vol. 18, no. 3, pp. 221—233.
Ohtaki, K., Kodama, Y., Nakano, M., Itoh, M., et al., Human fetuses do not register chromosome damage inflicted by radiation exposure in lymphoid precursor cells except for a small but significant effect at low doses, Radiat. Res., 2004, vol. 161, no. 4, pp. 373—379.
Akleyev, A., Deltour, I., Krestinina, L., Sokolnikov, M., et al., Incidence and mortality of solid cancers in people exposed in utero to ionizing radiation: pooled analyses of two cohorts from the Southern Urals, Russia, PLoS One, 2016, vol. 11, no. 8. https://doi.org/10.1371/journal.pone.0160372.eCollection
Alsbeih, G., Al-Meer, R.S., Al-Harbi, N., Bin Judia, S., et al., Gender bias in individual radiosensitivity and the association with genetic polymorphic variations, Radiother. Oncol., 2016, vol. 119, no. 2, pp. 236—243. https://doi.org/10.1016/j.radonc.2016.02.034
Morgan, W.F., Radiation-induced genomic instability, Health Phys., 2011, vol. 100, no. 3, pp. 280—281. https://doi.org/10.1097/HP.0b013e3182082f12
ACKNOWLEDGMENTS
We are grateful to Z.I. Sychenko for technical support of research. The work was supported by the Federal Biomedical Agency of Russia.
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Statement of compliance with standards of research involving humans as subjects. All procedures performed in a study involving people are in accordance with the ethical standards of the institutional and/or national research ethics committee and the 1964 Helsinki Declaration and its subsequent changes or comparable ethical standards. All participants in the study signed an informed consent.
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Akhmadullina, Y.R., Vozilova, A.V. & Akleyev, A.V. Study of the DNA Damage in Peripheral Blood Lymphocytes Using Micronucleus Test in Residents of the Techa Riverside Villages Who Were Chronically Exposed in Utero and Postnatally. Russ J Genet 56, 481–487 (2020). https://doi.org/10.1134/S102279542004002X
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DOI: https://doi.org/10.1134/S102279542004002X