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Identification of Imprinted Genes and Their Differentially Methylated Regions in Porcine

  • GENERAL GENETICS
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Abstract

Genomic imprinting is an epigenetic mechanism that leads to parent-specific gene expression. A number of imprinted genes have been identified in humans and mice but fewer genes have been described as imprinted in pig. In the study, 59 porcine candidate imprinted genes were obtained by bioinformatics analysis. Among them, Grb10, Slc22a3 and Slc22a18 were selected for molecular cloning and expression pattern analysis. And, single-nucleotide polymorphism-based methods were performed to identify their imprinting status. We found that Grb10 and Slc22a18 were imprinted in pig and their differentially methylated regions were further determined by bisulfite sequencing PCR. With this work we advance the field of genomic imprinting by expanding the list of imprinted genes in pig and demonstrate a feasible and effective method to characterize imprinted genes in mammalians.

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Funding

This work was supported by the National Transgenic Research Project (2016ZX08009003-006-008), the Young Talents Project of Northeast Agricultural University (14QC06), and the Open Projects of Key Laboratory of Animal Cellular and Genetic Engineering of Heilongjiang Province (KF201704).

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Authors and Affiliations

  1. Key Laboratory of Animal Cellular and Genetic Engineering of Heilongjiang Province, Northeast Agricultural University, 150030, Harbin, Heilongjiang, P.R. China

    Z. Yin, X. Zhang, J. Li, Y. Jiao, Q. Kong & Y. Mu

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  1. Z. Yin
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  2. X. Zhang
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  3. J. Li
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  4. Y. Jiao
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  5. Q. Kong
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  6. Y. Mu
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These authors contributed equally to this work.

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Correspondence to Q. Kong or Y. Mu.

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Conflict of interests. The authors declare that they have no conflict of interest.

Statement on the welfare of animals. All experiments were approved by the Animal Care Commission of Northeast Agriculture University, according to animal welfare laws, guidelines and policies.

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Yin, Z., Zhang, X., Li, J. et al. Identification of Imprinted Genes and Their Differentially Methylated Regions in Porcine. Russ J Genet 55, 1488–1498 (2019). https://doi.org/10.1134/S1022795419120135

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