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Deinococcus radiodurans RecX and Escherichia coli RecX proteins are capable to replace each other in vivo and in vitro

  • Molecular Genetics
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Abstract

A plasmid carrying the Deinococcus radiodurans recX gene under the control of a lactose promoter decreases the Escherichia coli cell resistance to UV irradiation and γ irradiation and also influences the conjugational recombination process. The D. radiodurans RecX protein functions in the Escherichia coli cells similarly to the E. coli RecX protein. Isolated and purified D. radiodurans RecX and E. coli RecX proteins are able to replace each other interacting with the E. coli RecA and D. radiodurans RecA proteins in vitro. Data obtained demonstrated that regulatory interaction of RecA and RecX proteins preserves a high degree of conservatism despite all the differences in the recombination reparation system between E. coli and D. radiodurans.

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Authors and Affiliations

  1. Konstantinov Petersburg Nuclear Physics Institute, National Research Center Kurchatov Institute, Gatchina, Leningrad oblast, 188300, Russia

    I. V. Bakhlanova & D. M. Baitin

  2. Peter the Great St. Petersburg Polytechnic University, St. Petersburg, 195251, Russia

    D. M. Baitin

Authors
  1. I. V. Bakhlanova
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  2. D. M. Baitin
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Correspondence to I. V. Bakhlanova.

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Original Russian Text © I.V. Bakhlanova, D.M. Baitin, 2016, published in Genetika, 2016, Vol. 52, No. 3, pp. 293–299.

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Bakhlanova, I.V., Baitin, D.M. Deinococcus radiodurans RecX and Escherichia coli RecX proteins are capable to replace each other in vivo and in vitro. Russ J Genet 52, 257–262 (2016). https://doi.org/10.1134/S1022795416030030

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  • DOI: https://doi.org/10.1134/S1022795416030030

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