Abstract
RecA/Rad51 family proteins assist in DNA repair. Current study was targeted on molecular cloning, expression in Escherichia coli and characterization of Pcal_2031, a RecA/Rad51 homologue from Pyrobaculum calidifontis. Recombinant Pcal_2031 was produced in soluble form in E. coli and exhibited high thermostability. Pcal_2031 displayed DNA binding ability in vitro and complemented ultraviolet light sensitivity in vivo. Primary structure analysis revealed the presence of two major conserved domains, the N-terminal helix-hairpin-helix (HHH_5) domain and the core RecA-like_NTPase domain. Deletion of HHH_5 domain resulted in loss of thermostability and a slight reduction in the enzyme activity of the recombinant protein. The reduction in thermostability may be attributed to the reduction in hydrogen bonds and salt bridges, predicted by in silico analysis. These results demonstrate that HHH_5 domain of Pcal_2031 might have a little role in maintaining enzyme function but provides additional structural stability to the protein.
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Abbas, S.N., Aslam, M., Maqsood, A. et al. Pcal_2031, a RecA/Rad51 homologue from Pyrobaculum calidifontis, complements the ultraviolet light sensitivity of Escherichia coli. Biologia 77, 3319–3326 (2022). https://doi.org/10.1007/s11756-022-01187-4
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DOI: https://doi.org/10.1007/s11756-022-01187-4