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Investigation of STAT5A, FSHR, and LHR gene polymorphisms in Turkish indigenous cattle breeds (East Anatolian Red, South Anatolian Red, Turkish Grey, Anatolian Black, and Zavot)

  • Animal Genetics
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Abstract

The objective of this study was to examine the allelic and genotypic profiles of the Signal Transducer and Activator of Transcription 5A (STAT5A), Follicle Stimulating Hormone Receptors (FSHR) and Luteinizing Hormone Receptor (LHR) genes in five indigenous cattle breeds in Turkey. For this purpose, a total of 329 cattle from East Anatolian Red (EAR), South Anatolian Red (SAR), Turkish Grey (TG), Anatolian Black (AB) and Zavot were genotyped using by PCR-RFLP method. A 215 bp fragment of STAT5A, a 306 bp fragment of FSHR and a 303 bp fragment of LHR were amplified and digested with AvaI, AluI, and HhaI restriction enzymes, respectively. In this study two types of alleles C and T for STAT5A, C and G for FSHR and C and T for LHR were observed. The highest frequencies for STAT5A-C and STAT5A-T alleles were estimated for the Zavot and TG breeds (0.86) and the EAR breed (0.29) respectively. The highest frequency for FSHR-C and FSHR-G alleles was estimated for the Zavot breed (0.72) and the AB and SAR breeds (0.35) respectively. The highest frequency for LHR-C and LHR-T alleles was estimated for the EAR breed (0.75) and the AB breed (0.39) respectively. According to F ST values, a small level of genetic diversity was found among five cattle breeds. The F ST value was calculated 0.019 between AB and Zavot. And, the value was significant (p < 0.001), while the other F ST values were not significant. According to the genetic distance values (Nei), the highest genetic distance was found between AB and TG while the smallest genetic distance was found between Zavot and TG. The chi-square test showed that the TG and Zavot breeds were in Hardy–Weinberg equilibrium (HWE) for STAT5A gene; the EAR, SAR, TG and Zavot breeds were in HWE for FSHR gene and the EAR, SAR and TG breeds were in HWE for LHR gene. In conclusion, further investigation is required to determine the correlation of the FSHR and LHR genes with early puberty for the improvement of sexual precocity, and it is considered that the STAT5A gene may be used to improve the milk yield and milk yield trails of local cattle breeds, including those indigenous to Turkey.

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Authors and Affiliations

  1. Department of Genetic, University of Erciyes, Kayseri, 38039, Turkey

    K. Arslan & B. Akyüz

  2. Department of Animal Science, University of Mehmet Akif Ersoy, Bardur, 15030, Turkey

    O. Korkmaz Agaoglu

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  1. K. Arslan
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  2. B. Akyüz
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  3. O. Korkmaz Agaoglu
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Correspondence to O. Korkmaz Agaoglu.

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Arslan, K., Akyüz, B. & Agaoglu, O.K. Investigation of STAT5A, FSHR, and LHR gene polymorphisms in Turkish indigenous cattle breeds (East Anatolian Red, South Anatolian Red, Turkish Grey, Anatolian Black, and Zavot). Russ J Genet 51, 1088–1095 (2015). https://doi.org/10.1134/S1022795415110022

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  • DOI: https://doi.org/10.1134/S1022795415110022

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