Abstract
The genetic diversity of 110 durum wheat genotypes of Azerbaijan was evaluated by ISSR markers. A total of 107 fragments were determined, ranging from 9 to 18 per locus. ISSR primers have revealed a high level of polymorphism (average 82%) among different durum wheat varieties and botanical varieties. The ISSR markers used in the study were quite informative and made it possible to distinguish all durum wheat accessions from each other. Cluster analysis based on ISSR data classified the accessions into 11 major groups. No linkage was observed between the collection site and genetic structure of the samples. On the other hand, a few accessions were detected as unique genotypes and tended to form separate clusters. The estimated gene diversity value was high, both within the whole collection and within the different groups of botanical varieties.
Similar content being viewed by others
References
Vavilov, N.I., Nauchnye osnovy selektsii pshenitsy (Scientific Bases of Wheat Breeding), Leningrad: Nauka, 1967.
Aliev, D.A. and Akperov, Z.I., Plant genetic resources of Azerbaijan, Vestn. Nats. Akad. Nauk Azerb., 2002, nos. 1–6, pp. 57–68.
Aliev, D.A., Akperov, Z.I., and Mamedov, A., Bioraznoobrazie (Biodiversity), Baku: Nauka, 2008.
Flyaksberger, K.F., Pshenitsa (Wheat), Moscow: Sel’khozgiz, 1938.
Kudryavtsev, A.M., Creation of genetic markers of durum wheat (T. durum Desf.) and their application in research and practical elaborations, Extended Abstract of Doctoral Dissertation, Inst. Obsh. Genet. Ross. Akad. Nauk, Moscow, 2007.
Aliyev, R.T., Abbasov, M.A., and Mammadov, A.C., Genetic identification of diploid and tetraploid wheat species with RAPD markers, Turk. J. Biol., 2007, vol. 31, pp. 173–180.
Savitskaya, V.A. and Gudinova, L.G., Selection of spring durum wheat: current state and the ways to improve drought resistance of varieties, Sel. Semenovod., 1985, no. 3, pp. 11–13.
Agarwal, M., Shrivastava, N., and Padh, H., Advances in molecular marker techniques and their applications in plant sciences, Plant Cell Rep., 2008, vol. 27, pp. 617–631.
Babayeva, S., Akparov, Z., Abbasov, M., et al., Diversity analysis of Central Asia and Caucasian lentil (Lens culinaris Medikus) germplasm using SSR fingerprinting, Genet. Res. Crop. Evol., 2009, vol. 56, pp. 293–298.
Hou, Y.C., Yan, Z.H., Wei, Y.M., and Zheng, Y.L., Genetic diversity in barley from west China based on RAPD and ISSR analysis, Barley Genet. Newslett., 2005, vol. 35, pp. 9–12.
Liao, L., Guo, Q., Wang, Z., et al., Genetic diversity analysis of Prunella vulgaris in China using ISSR and SRAP markers, Biochem. System. Ecol., 2012, vol. 45, pp. 209–217.
Karaca, M. and Izbirak, A., Comparative analysis of genetic diversity in Turkish durum wheat cultivars using RAPD and ISSR markers, J. Food Agric. Environ., 2008, vol. 6, pp. 219–225.
El-Assal, S.E.D. and Gaber, A., Discrimination capacity of RAPD, ISSR and SSR markers and their effectiveness in establishing genetic relationship and diversity among Egyptian and Saudi wheat cultivars, Am. J. Appl. Sci., 2012, vol. 9, pp. 724–735.
Boboshina, I.V. and Boronnikova, S.V., Identification of promising for Urals soft wheat varieties using microsatellite ISSR-analysis of DNA polymorphism, Fundam. Issled., 2013, no. 6, pp. 92–97.
zzatullayeva V.İ., Babayeva, S.M., Akparov, Z.I., et al., Efficiency of using RAPD and ISSR markers in evaluation of genetic diversity in sugar beet, Turk. J. Biol., 2014, vol. 38, pp. 429–438.
Doyle, J.L. and Doyle, J.J., A rapid DNA isolation procedure for small quantities of fresh leaf tissue, Phytochem. Bull., 1987, vol. 19, pp. 11–15.
Hämmer, Ø., Harper, D.A.T. and Ryan, P.D., Past: paleontological statistics software package for education and data analysis, Palaeontol. Electron., 2001, vol. 4, pp. 1–9.
Weir, B.S., Genetic-Data Analysis Methods for Discrete Genetic Data, Sunderland, MA: Sinauer, 1990.
Sofalian, O., Chaparzadeh, N., Javanmard, A., and Hejazi, S., Study the genetic diversity of wheat landraces from northwest of Iran based on ISSR molecular markers, Int. J. Agric. Biol., 2008, vol. 10, pp. 466–468.
Zhu, S., Hu, J., Han, R., et al., Fingerprinting and identification of closely related wheat (Triticum aestivum L.) cultivars using ISSR and fluorescence-labeled TP-M13-SSR markers, Aust. J. Crop Sci., 2011, vol. 5, no. 7, pp. 846–850.
Carvalho, A., Brito, J.L., Macas, B., and Pinto, H.G., Genetic diversity and variation among botanical varieties of old Portuguese wheat cultivars revealed by ISSR assays, Biochem. Genet., 2009, vol. 47, pp. 276–294.
Song, Q.J., Fichus, E.W., and Cregan, P.B., Characterization of trinucleotide SSR motifs in wheat, Theor. Appl. Genet., 2002, vol. 104, pp. 286–293.
Nagoaka, T. and Ogihara, Y., Applicability of intersimple sequence repeat polymorphisms in wheat for use as DNA markers in comparison to RFLP and RAPD markers, Theor. Appl. Genet., 1997, vol. 94, pp. 597–602.
Akkaya, M.S., Bhagwat, A.A., and Cregan, P.B., Length polymorphisms of simple sequence repeat DNA in soybean, Genetics, 1992, vol. 132, pp. 1131–1139.
Pasqualone, A., Lotti, C., Bruno, A., Vita, P., et al., Use of ISSR markers for cultivar identification in durum wheat, Options Mediterr., Ser. A. Sem. Medit., 2000, vol. 40, pp. 157–161.
Sofalian, O., Chaparzadeh, N., and Dolati, M., Genetic diversity in spring wheat landraces from northwest of Iran assessed by ISSR markers, Notul. Bot. Hort. Agric. Cluj-Napoca, 2009, vol. 37, pp. 252–256.
Najaphy, A., Parchin, R.A., and Farshadfar, E., Comparison of phenotypic and molecular characterizations of some important wheat cultivars and advanced breeding lines, Aust. J. Crop Sci., 2012, vol. 6, pp. 326–332.
Tishchenko, V.N., Chekalin, N.M., and Zyukov, M.E., The use of cluster analysis for the identification and selection of highly productive genotypes of winter wheat during the early stages of selection, in Faktori eksperimental’noi evolyutsii organizmiv (Factors of Experimental Evolution of Organisms), Киïв: Agrarna Nauka, 2004, vol. 2, pp. 270–278.
Carvalho, A., Brito, J.L., Macas, B., and Pinto, H.G., Genetic variability analysis of a collection of old Portuguese bread wheat using ISSRS, Options Mediterr., Ser. A. Sem. Medit., 2008, vol. 81, pp. 35–38.
Malik, R., Sareen, S., Kundu, S., and Shoran, J., The use of SSR and ISSR markers for assessing DNA polymorphism and genetic diversity among Indian bread wheat cultivars, Prog. Agric., 2010, vol. 12, pp. 82–89.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © E.S. Hajiyev, Z.I. Akparov, R.T. Aliyev, S.V. Saidova, V.I. Izzatullayeva, S.M. Babayeva, M.A. Abbasov, 2015, published in Genetika, 2015, Vol. 51, No. 9, pp. 1009–1017.
Rights and permissions
About this article
Cite this article
Hajiyev, E.S., Akparov, Z.I., Aliyev, R.T. et al. Genetic polymorphism of durum wheat (Triticum durum Desf.) accessions of azerbaijan. Russ J Genet 51, 863–870 (2015). https://doi.org/10.1134/S1022795415090045
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1022795415090045