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Study of chloroplast DNA polymorphism in the sunflower (Helianthus L.)

  • Plant Genetics
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Abstract

The polymorphism of microsatellite loci of chloroplast genome in six Helianthus species and 46 lines of cultivated sunflower H. annuus (17 CMS lines and 29 Rf-lines) were studied. The differences between species are confined to four SSR loci. Within cultivated forms of the sunflower H. annuus, the polymorphism is absent. A comparative analysis was performed on sequences of the cpDNA inbred line 3629, line 398941 of the wild sunflower, and the American line HA383 H. annuus. As a result, 52 polymorphic loci represented by 27 SSR and 25 SNP were found; they can be used for genotyping of H. annuus samples, including cultural varieties: twelve polymorphic positions, of which eight are SSR and four are SNP.

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Authors and Affiliations

  1. Department of Genetics, Southern Federal University, Rostov-on-Don, 344090, Russia

    N. V. Markin, A. V. Usatov, K. V. Azarin, I. V. Kornienko & V. E. Tihobaeva

  2. Belozersky Institute of Physical and Chemical Biology, Lomonosov Moscow State University, Moscow, 119992, Russia

    M. D. Logacheva

  3. All-Russia Research Institute of Oil Crops, Zhdanov Don Experiment Station, pos. Oporny, Rostov oblast, 346754, Russia

    O. F. Gorbachenko

  4. Vavilov Research Institute of Plant Industry, St. Petersburg, 190000, Russia

    V. A. Gavrilova

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  1. N. V. Markin
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  2. A. V. Usatov
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  3. M. D. Logacheva
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  4. K. V. Azarin
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  5. O. F. Gorbachenko
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  6. I. V. Kornienko
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  7. V. A. Gavrilova
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  8. V. E. Tihobaeva
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Correspondence to N. V. Markin.

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Original Russian Text © N.V. Markin, A.V. Usatov, M.D. Logacheva, K.V. Azarin, O.F. Gorbachenko, I.V. Kornienko, V.A. Gavrilova, V.E. Tihobaeva, 2015, published in Genetika, 2015, Vol. 51, No. 8, pp. 873–880.

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Markin, N.V., Usatov, A.V., Logacheva, M.D. et al. Study of chloroplast DNA polymorphism in the sunflower (Helianthus L.). Russ J Genet 51, 745–751 (2015). https://doi.org/10.1134/S1022795415060101

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  • DOI: https://doi.org/10.1134/S1022795415060101

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