Abstract
Transgenic tobacco plants expressing Arabidopsis thaliana ARGOS and ARGOS-LIKE genes under the control of the chalcone synthase promoter of Petunia hybrida L., as well as the estradiol inducible XVE system, have been obtained. The part of transgenic plants with flower-specific expression of the target genes was characterized by increased flower size, caused by an increase in cell size and quantity in the case of the ARGOS gene and by a stimulation of cell growth via stretching in the case of the ARGOS-LIKE gene. An enhanced expression level of the NtEXPA1, NtEXPA4 genes encoding expansins, NtEXGT gene encoding endo-xyloglucan transferase, and the AINTEGUMENTA-like gene was detected in the flowers of transgenic tobacco plants. In the case of inducible expression of ARGOS and ARGOS-LIKE genes, an increase in leaf, stem and flower size was revealed in several lines of transgenic plants as compared to control. Expression of the ARGOS gene also affected cell number and size in this case, while the ARGOS-LIKE gene mainly influenced cell size via stretching. Inducible expression of the ARGOS gene in flowers mainly provided an enhanced containment of AINTEGUMENTA-like mRNA, while ARGOS-LIKE gene expression resulted in the activation of NtEXPA1 and NtEXGT genes.
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Gonzalez, N., Vanhaeren, H., and Inze, D., Leaf size control: complex coordination of cell division and expansion, Trends Plant Sci., 2012, vol. 17, pp. 332–340.
Tsukaya, H. and Beemster, G.T., Genetics, cell cycle and cell expansion in organogenesis in plants, J. Plant Res., 2006, vol. 119, pp. 1–4.
Krizek, B.A., Ectopic expression of AINTEGUMENTA in Arabidopsis plants results in increased growth of floral organs, Dev. Genet., 1999, vol. 25, pp. 224–236.
Wang, B., Sang, Y., Song, J., et al., Expression of rice OsARGOS gene in Arabidopsis promotes cell division and expansion and increases organ size, J. Genet. Genomics, 2009, vol. 36, pp. 31–40.
Feng, G., Qin, Z., Yan, J., et al., Arabidopsis ORGAN SIZE RELATED1 regulates organ growth and final organ size in orchestration with ARGOS and ARL, New Phytol., 2011, vol. 191, pp. 635–646.
Hu, Y., Xie, Q., and Chua, N., The Arabidopsis auxininducible gene ARGOS controls lateral organ size, Plant Cell, 2003, vol. 15, pp. 1951–1961.
Wang, B., Zhou, X., Xu, F., and Gao, J., Ectopic expression of a Chinese cabbage BrARGOS gene in Arabidopsis increases organ size, Transgenic Res., 2009, vol. 19, pp. 461–472.
Mizukami, Y. and Fischer, R.L., Plant organ size control: AINTEGUMENTA regulates growth and cell numbers during organogenesis, Proc. Natl. Acad. Sci. U.S.A., 2000, vol. 97, pp. 942–947.
Hu, Y., Poh, H., and Chua, N., The Arabidopsis ARGOS-LIKE gene regulates cell expansion during organ growth, Plant J., 2006, vol. 47, pp. 1–9.
Kuluev, B.R., Knyazev, A.V., Il’yasova, A.A., and Chemeris, A.V., Constitutive expression of the ARGOS gene driven by dahlia mosaic virus promoter in tobacco plants, Russ. J. Plant Physiol., 2011, vol. 58, no. 3, pp. 507–515.
Kuluev, B.R., Knyazev, A.V., Safiullina, M.G., and Chemeris, A.V., Effect of constitutive expression of ARGOS-LIKE gene on sizes of cells and organs of transgenic tobacco plants, Russ. J. Genet., 2013, vol. 49, no. 5, pp. 503–510.
van der Meer, I.M., Spelt, C.E., Mol, J.N., and Stuitje, A.R., Promoter analysis of the chalcone synthase (chsA) gene of Petunia hybrida: a 67 bp promoter region directs flower-specific expression, Plant Mol. Biol., 1990, vol. 15, pp. 95–109.
Han, Y., Ming, F., Wang, J., et al., A novel chalcone synthase gene from Phalaenopsis orchid that alters floral morphology in transgenic tobacco plants, Plant Mol. Biol. Rep., 2005, vol. 23, pp. 193–194.
Aoyama, T. and Chua, N.H., A glucocorticoid-mediated transcriptional induction system in transgenic plants, Plant J., 1997, vol. 11, pp. 605–612.
Zuo, J., Niu, Q.W., and Chua, N.H., Technical advance: an estrogen receptor-based transactivator XVE mediates highly inducible gene expression in transgenic plants, Plant J., 2000, vol. 24, pp. 265–273.
Link, B.M. and Cosgrove, D.J., Acid-growth response and α-expansins in suspension cultures of bright yellow 2 tobacco, Plant Physiol., 1998, vol. 118, pp. 907–916.
Rieu, I., Bots, M., Mariani, C., and Weterings, K.A., Isolation and expression analysis of a tobacco AIN-TEGUMENTA ortholog (NtANTL), Plant Cell Physiol., 2005, vol. 46, pp. 803–805.
Livak, K.J. and Schmittgen, T.D., Analysis of relative gene expression data using real-time quantitative PCR and the method, Methods, 2001, vol. 25, pp. 402–408.
Verdonk, J.C., Shibuya, K., Loucas, H.M., et al., Flower-specific expression of the Agrobacterium tumefaciens isopentenyltransferase gene results in radial expansion of floral organs in Petunia hybrida, Plant Biotechnol. J., 2008, vol. 6, pp. 694–701.
Mitsuhara, I., Ugaki, M., and Hirochika, H., Efficient promoter cassettes for enhancer expression of foreign genes in dicotyledonous and monocotyledonous plants, Plant Cell Physiol., 1996, vol. 37, pp. 49–59.
Fritze, K., Staiger, D., Czaja, I., et al., Developmental and UV light regulation of the snapdragon chalcone synthase promoter, Plant Cell, 1991, vol. 3, pp. 893–905.
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Original Russian Text © B.R. Kuluev, A.V. Knyazev, Yu.M. Nikonorov, A.V. Chemeris, 2014, published in Genetika, 2014, Vol. 50, No. 8, pp. 918–929.
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Kuluev, B.R., Knyazev, A.V., Nikonorov, Y.M. et al. Estradiol inducible and flower-specific expression of ARGOS and ARGOS-LIKE genes in transgenic tobacco plants. Russ J Genet 50, 807–817 (2014). https://doi.org/10.1134/S1022795414070102
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DOI: https://doi.org/10.1134/S1022795414070102