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Transformation of LRP gene into Brassica napus mediated by agrobacterium tumefaciens to enhance lysine content in seeds

  • Plant Genetics
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Abstract

Lysine rich protein (LRP) gene derived from the seed of Psophocarpus tetragonolobus was transformed into Brassica napus, employing cotyledon petiole as explants and by using the Agrobacterium tumefaciens strain LBA4404. Transformation efficiency was found to be closely related with phytohormone concentration, infection incubation, and co-cultured time. A medium containing 4 mg/l 6-benzyladenine (6-BA) and 0.3 mg/1 naphthalene acetic acid (NAA) was used for plant regeneration. With infection incubation of A. tumefaciens (OD600 = 0.4) for 20 min and co-culture of infected cotyledon petiole for 3 days, the highest transformation efficiency of 8.5% was obtained. To confirm LRP gene expression, PCR and Southern blot analysis were performed on leaf-isolated DNA from regenerated plants resistant to kanamycin. All transgenic plants of the generation T0 formed fertile seeds, which were sowed for the inheritance study of generational T1 and amino acid analysis. It was found that the lysine content of seeds from T1 generation increased by 16.7% compared with non-transgenic lines.

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Authors and Affiliations

  1. College of Bioscience and Biotechnology, Yangzhou University, Yangzhou, 225009, China

    J. Wang, L. Chen & Y. P. Wang

  2. Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology, Yangzhou University, Yangzhou, 225009, China

    Q. Q. Liu

  3. Department of Biology, Chinese University of Hong Kong, Hong Kong, China

    S. S. M. Sun

  4. Institute of Molecular & Cell Biology, Siberian Branch, RAS, Novosibirsk, 630090, Russia

    V. Sokolov

  5. N. I. Vavilov Institute of Plant Industry, St. Petersburg, 190000, Russia

    V. Sokolov

Authors
  1. J. Wang
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  2. L. Chen
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  3. Q. Q. Liu
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  4. S. S. M. Sun
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  5. V. Sokolov
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  6. Y. P. Wang
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Correspondence to V. Sokolov.

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Wang, J., Chen, L., Liu, Q.Q. et al. Transformation of LRP gene into Brassica napus mediated by agrobacterium tumefaciens to enhance lysine content in seeds. Russ J Genet 47, 1433–1437 (2011). https://doi.org/10.1134/S1022795411120167

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  • DOI: https://doi.org/10.1134/S1022795411120167

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