Abstract
Maize Research Institute (MRI) gene bank maintains a collection of 6000 maize accessions. Within this collection over 100 sources of cytoplasmic male sterility (CMS) were found in field trials, i.e. more than 2% of the total accession numbers. These sources are distributed among Yugoslav open-pollinated varieties (4.56% of them contain CMS), as well as introduced heterozygous genotypes and inbred lines. In order to identify cytoplasm types the gene-bank sources of CMS were screened using a PCR assay with specific primers for C, T and S cytoplasms. Predominant cytoplasmic male sterility type among the analyzed accessions was CMS-S. Results were inconclusive for three accessions, i.e. different results for the progenies of two ears per accession were obtained. For another two accessions a new PCR product profile was identified, consisting of one band characteristic for CMS-S and one unspecific for any of the three CMS types. The PCR approach enabled a simple, fast and reliable large scale screening of maize cytoplasm among MRI gene bank accessions, significantly reducing time for cytoplasm characterizations compared to classical method of testing with restorers for each known type of CMS.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Kaul, M.L.H., Male Sterility in Higher Plants, Berlin: Springer-Verlag, 1988.
Hermsen, J.G.Th., Towards a More Efficient Utilization of Genic Male Sterility in breeding Hybrid Barley and Wheat, Euphytica, 1965, vol. 14, pp. 221–224.
Hermsen, J.G.Th., A Discussion on Cytoplasmic Restoration of ms-Sterility, Euphytica, 1968, suppl. 1, pp. 63–67.
Kohel, R.J., and Richmond, T.R, Tests for Cytoplasmic-Genetic Interaction Involving a Genetic Male Sterile Stock of the Genotype ms2ms2, Crop Sci., 1963, vol. 3, pp. 361–362.
Rutger, J.N. and Jensen, N.F., On the Use of Genetic Male Sterility in Screening for Cytoplasmic-Genetic Sterility in Barley, Euphytica, 1967, vol. 16, pp. 350–353.
Washnok, R.F., Cytoplasmic Restoration of ms-Sterility, MNL, 1972, vol. 46, pp. 25–27.
Vidakovic, M., Vidakovic, M., and Vančetović, J., A New Search for Restorer Cytoplasm: The Restorer Cytoplasm for the Gene ms10 Most Probably Does not Exist in Maize, J. Heredity, 2002, vol. 93, no. 6, pp. 444–447.
Schnable, P.S. and Wise, R.P., The Molecular Basis of Cytoplasmic Male Sterility and Fertility Restoration, Trends Plant Sci., 1998, vol. 3, pp. 175–180.
Dewey, R.E., Levings, C.S.III, and Timothy, D.H., Novel Recombinations in the Maize Mitochondrial Genome Produce a Unique Transcription Unit in the Texas Male Sterile Cytoplasm, Cell, 1986, vol. 44, pp.439–449.
Dewey, R.E., Timothy, D.H., and Levings, C.S.III, Chimeric Mitochondrial Genes Expressed in the C Male Sterile Cytoplasm of Maize, Curr. Genet., 1991, vol. 20, pp. 475–482.
Zabala, G., Gabay-Laughnan, S., and Laughnan, J.R., The Nuclear Gene Rf3 Affects the Expression of the Mitochondrial Chimeric Sequence R in S-Type Male Sterility in Maize, Genetics, 1997, vol. 147, pp. 847–860.
Liu, Z., Peter, S.O., Long, M.U., et al., A PCR Assay for Rapid Discrimination of Sterile Cytoplasm Types in Maize, Crop Sci., 2002, vol. 42, pp. 566–569.
Saghai-Maroof, M., Soliman, K., Jorgensen, R., and Allard, R., Ribosomal DNA Spacer Length Polymorphism in Barley: Mendelian Inheritance, Chromosomal Location and Population Dynamics, Proc. Natl. Acad. Sci. USA, 1984, vol. 91, pp. 8014–8018.
Levings, S.C., The Texas Cytoplasm of Maize: Cytoplasmic Male Sterility and Disease Susceptibility, Science, 1990, vol. 250, no. 4983, pp. 942–947.
Goodman, M.M., Genetic and Germplasm Stocks Worth Conserving, J. Heredity, 1990, vol. 81, pp. 11–16.
Nass, L.L. and Paterniani, E., Pre-Breeding: A Link between Genetic Resources and Maize Breeding, Scientia Agricola, 2000, vol. 57, no. 3, pp. 581–587.
Wei, G.W. and Zheng, Y.L., mtDNA Heterogeneity of Cytoplasmic Male Sterility in Maize, Acta Genet. Sin., 1997, vol. 24, pp. 66–77.
Escote, L.J., Gubay-Laughnan, S.J., and Laughnan, J.R., Cytoplasmic Reversion to Fertility in cms-S Maize Need not Involve Loss of Linear Mitochondrial Plasmids, Plasmid, 1985, vol. 14, no. 3, pp. 264–267.
Vančetović, J., Ignjatović-Micić, D., Nikolić, A., and Branković, D., The Maize Variety Acc. no. 366—Yellow Dent from the Region of Novi Sad—from the Former Yugoslav MRI Collection Caries a T Cytoplasmic Male Sterility (Tms), in Plant Genetic Stocks—the Basis of Agriculture of Today, Int. Res. Conf., Plovdiv, 2007.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © J. Vančetović, M. Vidaković, D. Ignjatović-Micić, A. Nikolić, K. Marković, V. Anđelković, 2010, published in Genetika, 2010, Vol. 46, No. 7, pp. 940–944.
The article is published in the original.
Rights and permissions
About this article
Cite this article
Vančetović, J., Vidaković, M., Ignjatović-Micić, D. et al. The structure of sterile cytoplasm types within a maize genebank collection. Russ J Genet 46, 836–840 (2010). https://doi.org/10.1134/S1022795410070082
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1022795410070082