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Evaluation of genetic variability in choosen apple (Malus × domestica Borkh.) cultivars by ISSR-PCR analysis

  • Plant Genetics
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Abstract

The aim of the study was to determine the genetic variability in eight apple cultivars: Delikates, Cortland, James Grieve, Lired, Jonathan, Golden Delicious, Jonagold, and Idared from the collection of Fruit Growing Research Station in Rajkowo of the West Pomeranian University of Technology, Szczecin. The cultivar Delikates was obtained from the crossing of two cultivars: Cortland, and James Grieve, whereas cultivar Lired is a James Grieve’s sport. The second one cultivar—Jonagold was obtained from the crossing of Jonathan and Golden Delicious. The cultivar Idared is a hybrid obtained from the crossing of Jonathan and Wagener. Out of 40 primers, 17 were chosen for the final study. Those amplified a total of 183 loci (872 amplicons) out of which 34 (18.5%) were monomorphic, 128 (69.5%) were polymorphic and 22 (12%) cultivar-specific. Specific ISSR products were detected for each apple cultivar. A dendrogram was constructed using the UPGMA method which revealed two distinct clusters: I—Delikates, Cortland, James Grieve and Lired, II—Jonathan, Golden Delicious, Jonagold and Idared. Genetic similarity between Delikates, Cortland and James Grieve was 68.6, 70.8%, respectively and between cultivar Jonagold, Jonathan and Golden Delicous was 79.8, 85.2%, respectively.

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References

  1. Evans, C.R. and Campbell, C.S., The Origin of the Apple Subfamily (Maloideae; Rosaceae) Is Clarified by DNA Sequence Data from Duplicated GBSSI Genes, Amer. J. Botany, 2002, vol. 89, no. 9, pp. 1478–1484.

    Article  CAS  Google Scholar 

  2. Baldi, P., Patocchi, A., Zini, E., et al., Cloning and Linkage Mapping of Resistance Gene Homologues in Apple, Theor. Appl. Genet., 2004, vol. 109, no. 1, pp. 231–239.

    Article  CAS  PubMed  Google Scholar 

  3. Royo, J.B. and Itoiz, R., Evaluation of the Discriminance Capacity of RAPD, Isoenzymes and Morphologic Markers in Apple (Malus × domestica Borkh.) and the Congruence among Classifications, Genet. Res. Crop Evol., 2004, vol. 51, pp. 153–160.

    Article  Google Scholar 

  4. Ziętkiewicz, E., Rafalski, A., and Labuda, D., Genome Fingerprinting by Simple Sequence Repeat (SSR)-Anchored Polymerase Chain Reaction Amplification, Genomics, 1994, vol. 20, pp. 176–183.

    Article  PubMed  Google Scholar 

  5. PradeepReddy, M.P., Sarla, N., Siddig, E.A., Inter Simple Sequence Repeat (ISSR) Polymorphism and Its Application in Plant Breeding, Euphytica, 2002, vol. 128, pp. 9–17.

    Article  CAS  Google Scholar 

  6. Levinson, G. and Gutman, G.A., Slipped-Strand Mispairing: A Major Mechanism for DNA Sequence Evolution, Mol. Biol. Evol., 1987, vol. 4, pp. 203–221.

    CAS  PubMed  Google Scholar 

  7. Wang, G., Mahalingan, R., and Knap, H.T., (C-A) and (G-A) Anchored Simple Sequence Repeats (ASSRs) Generated Polymorphism in Soybean, Theor. Appl. Genet., 1998, vol. 96, pp. 1086–1096.

    Article  CAS  Google Scholar 

  8. Hadonou, A.M., Gittins, J.R., Hiles, E.R., and James, D.J., Two Apple Repetitive Sequences Elements: Characterisation and Potential Use as Genetic Markers, Euphytica, 2003, vol. 131, no. 2, pp. 177–187.

    Article  CAS  Google Scholar 

  9. Nei, M. and Li, W.H., Mathematical Model for Studying Genetic Variation in Terms of Restriction Endonucleases, Proc. Nat. Acad. Sci. USA, 1979, vol. 76, pp. 5269–5273.

    Article  CAS  PubMed  Google Scholar 

  10. Van de Peer, Y. and De Wachter, R., TREECON for Windows: A Software Package for the Construction and Drawing of Evolutionary Trees for the Microsoft Windows Environment, Computer Appl. Biosci., 1994, vol. 10, pp. 569–570.

    Google Scholar 

  11. Wu, K.S., Jonas, R., Dauneberger, L., and Scolnik, B.A., Detection of Microsatellite Polymorphism without Cloning, Nucleic Acids Res., 1994, vol. 22, pp. 3257–3258.

    Article  CAS  PubMed  Google Scholar 

  12. Akagi, H., Yokozeki, Y., Inagaki, A., et al., A Codominant DNA Marker Closely Linked to the Rice Nuclear Restorer Gene, Rf-1, Identified with Inter-SSR Fingerprinting, Genome, 1996, vol. 39, pp. 1205–1209.

    Article  CAS  PubMed  Google Scholar 

  13. Wang, Z., Weber, J.K., Zhong, G., and Tanksley, D.S., Survey of Plant Short Tandem DNA Repeats, Theor. Appl. Genet., 1994, vol. 88, pp. 1–6.

    CAS  Google Scholar 

  14. Tsumura, Y., Ohba, K., and Strauss, S., Diversity and Inheritance of Inter-Simple Sequence Repeat Polymorphisms in Douglas-Fir (Pseudotsuga menziesii) and Sugi (Cryptomeria japonica), Theor. Appl. Genet., 1996, vol. 92, pp. 40–45.

    Article  CAS  Google Scholar 

  15. Ratnaparkhe, M.B., Santra, D.K., Tullu, A., and Muehlbauer, F.J., Inheritance of Inter-Simple Sequence Repeat Polymorphisms and Linkage with a Fusarium Wilt Resistance Gene in Chickpea, Theor. Appl. Genet., 1998, vol. 96, pp. 348–353.

    Article  CAS  Google Scholar 

  16. Joshi, S.P., Gupta, V.S., Aggarwal, R.K., et al., Genetic Diversity and Phylogenetic Relationship as Revealed by Inter Simple Sequence Repeat (ISSR) Polymorphism in the Genus Oryza, Theor. Appl. Genet., 2000, vol. 100, pp. 1311–1320.

    Article  CAS  Google Scholar 

  17. Kochieva, E.Z., Ryzhova, N.N., Khrapalova, I.A., and Pukhalskyi, V.A., Genetic Diversity and Phylogenetic Relationships in the Genus Lycopersicon as Revealed by Inter-Simple Sequence Repeat (ISSR) Analysis, Russ. J. Genet., 2002, vol. 38, no. 8, pp. 958–966.

    Article  CAS  Google Scholar 

  18. Tikunov, Yu.M., Khrustaleva, L.J., and Karlo, G.J., Application of ISSR Markers in the Genus Lycopersicon, Euphytica, 2003, vol. 131, pp. 71–80.

    Article  CAS  Google Scholar 

  19. Gianfranceschi, L., Seglias, N., Tarchini, R., et al., Simple Sequence Repeats for the Genetic Analysis of Apple, Theor. Appl. Genet., 1998, vol. 96, pp. 1069–1076.

    Article  CAS  Google Scholar 

  20. Goulão, L. and Oliveira, C.M., Molecular Characterisation of Cultivars of Apple (Malus × domestica Borkh.) Using Microsatellite (SSR and ISSR) Markers, Euphytica, 2001, vol. 122, pp. 81–89.

    Article  Google Scholar 

  21. Zhu, Y.D., Zhang, W., Li, G.C., and Wang, T., Evaluation of Inter-Simple Sequence Repeat Analysis for Mapping the Co Genes in Apple (Malus pumila Mill.), Horticultural Sci. Biotechnol., 2007, vol. 82, no. 3, pp. 371–376.

    CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Horticultural Plant Breeding, West Pomeranian University of Technology, Szczecin, 71-424, Poland

    M. Smolik & O. Krzysztoszek

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  1. M. Smolik
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  2. O. Krzysztoszek
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Correspondence to M. Smolik.

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Original Russian Text © M. Smolik, O. Krzysztoszek, 2010, published in Genetika, 2010, Vol. 46, No. 7, pp. 923–931.

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Smolik, M., Krzysztoszek, O. Evaluation of genetic variability in choosen apple (Malus × domestica Borkh.) cultivars by ISSR-PCR analysis. Russ J Genet 46, 819–827 (2010). https://doi.org/10.1134/S1022795410070069

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  • DOI: https://doi.org/10.1134/S1022795410070069

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